公开(公告)号：US20150196915A1

公开(公告)日：2015-07-16

申请号：US14669419

申请日：2015-03-26

Applicant: GEN-PROBE INCORPORATED

Inventor： Norbert D. HAGEN ,  Byron J. KNIGHT ,  David OPALSKY

IPC: B01L3/00 ,  B01L9/00

CPC classification number: B01L3/52 ,  B01L3/561 ,  B01L9/52 ,  B01L2300/0609 ,  B01L2300/12 ,  B03C1/0332 ,  B03C1/12 ,  B03C1/14 ,  B03C1/28 ,  B03C1/286 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/26 ,  B25J11/00 ,  B65B3/04 ,  C12N15/1013 ,  G01N35/0098 ,  Y10T29/49815

Abstract: Methods, systems, and apparatus are provided for automated isolation of selected analytes, to which magnetically-responsive solid supports are bound, from other components of a sample. An apparatus for performing an automated magnetic separation procedure includes a mechanism for effecting linear movement of a magnet between operative and non-operative positions with respect to a receptacle device. A receptacle holding station within which a receptacle device may be temporarily stored prior to moving the receptacle to the apparatus for performing magnetic separation includes magnets for applying a magnetic field to the receptacle device held therein, thereby drawing at least a proton of the magnetically-responsive solid supports out of suspension before the receptacle device is moved to the magnetic separation station. An automated receptacle transport mechanism moves the receptacle devices between the apparatus for performing magnetic separation and the receptacle holding station.

公开(公告)号：US20150182708A1

公开(公告)日：2015-07-02

申请号：US14536773

申请日：2014-11-10

Applicant: Joshua C. Barnard

Inventor： Joshua C. Barnard

IPC: A61M13/00 ,  B03C3/017

CPC classification number: A61M13/003 ,  A61B17/3421 ,  A61B2218/006 ,  A61B2218/008 ,  A61M2202/0225 ,  A61M2205/3317 ,  A61M2205/75 ,  A61M2205/7545 ,  B03C3/017 ,  B03C3/32 ,  B03C3/41 ,  B03C3/49 ,  B03C3/60 ,  B03C2201/10 ,  B03C2201/26

Abstract: A device for the mitigation of smoke and particulate suspended in an insufflated body cavity during minimally invasive surgical procedures consist of a single electrode manufactured within and/or on a trocar shaft, or any other laparoscopic or endoscopic instrument. The electrode is made of any electrically active material with many hundreds or thousands of extremely fine terminal points, such as carbon fiber or laser etched metals. The electrode is electrically connectable to the neutral (negative) pole of a source of high voltage direct current (DC) electricity and the positive pole of the same electrical source is grounded or terminally insulated. When the positive electrical pole is connected to the ground and the carbon fiber or etched metal electrode is connected the negative pole, anions stream from the terminal ends of the terminal ends attracting smoke, and any other particulate suspended in the cavity, causing them to precipitate out of the suspending gas and therefore clearing the surgeon's view.

Abstract translation: 在微创外科手术过程中用于减轻悬挂在吹入体腔内的烟雾和微粒的装置由在套管针或其他腹腔镜或内窥镜器械内部和/或之上制造的单个电极组成。 电极由具有数百或数千个非常细的端点的任何电活性材料制成，例如碳纤维或激光蚀刻金属。 电极可电连接到高压直流（DC）电源的中性（负极），同一电源的正极接地或终端绝缘。 当正电极连接到地面并且碳纤维或蚀刻的金属电极连接到负极时，来自终端末端的阴离子流吸引烟雾，并且悬浮在空腔中的任何其它颗粒导致它们沉淀 因此清除外科医生的意见。

公开(公告)号：US20150160116A1

公开(公告)日：2015-06-11

申请号：US14590659

申请日：2015-01-06

Applicant: Becton, Dickinson and Company

Inventor： Liping Yu ,  Brian David Warner ,  Joseph T. Trotter

IPC: G01N15/14

CPC classification number: G01N15/1404 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0877 ,  B01L2400/043 ,  B01L2400/0439 ,  B03C1/02 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/26 ,  G01N1/34 ,  G01N1/4077 ,  G01N15/02 ,  G01N15/1056 ,  G01N15/14 ,  G01N15/1425 ,  G01N15/1459 ,  G01N33/54326 ,  G01N2001/4038 ,  G01N2001/4094 ,  G01N2015/008 ,  G01N2015/0288 ,  G01N2015/1006 ,  G01N2015/142 ,  G01N2015/149

Abstract: Devices for sorting components (e.g., cells) contained in a liquid sample are provided. In certain aspects, the devices include a magnetic separation device and an acoustic concentrator device fluidically coupled to magnetic separation device. Aspects of the invention further include methods for sorting cells in a liquid sample, and systems, and kits for practicing the subject methods.

Abstract translation: 提供用于分选液体样品中包含的组分（例如，细胞）的装置。 在某些方面，这些装置包括磁分离装置和流体耦合到磁分离装置的声聚集装置。 本发明的方面还包括用于分选液体样品中的细胞的方法，以及用于实践本发明方法的系统和试剂盒。

公开(公告)号：US09050606B2

公开(公告)日：2015-06-09

申请号：US14491231

申请日：2014-09-19

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Vamsee K. Pamula ,  Arjun Sudarsan ,  Ramakrishna Sista

IPC: G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  B03C5/00 ,  G01N33/543 ,  B03C1/01 ,  B03C1/28 ,  B03C5/02

CPC classification number: B03C7/026 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0887 ,  B01L2400/0424 ,  B01L2400/0427 ,  B03C1/01 ,  B03C1/288 ,  B03C5/00 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  G01N33/54326

Abstract: The invention provides a method of redistributing magnetically responsive beads in a droplet. The method may include conducting on a droplet operations surface one or more droplet operations using the droplet without removing the magnetically responsive beads from the region of the magnetic field. The droplet operations may in some cases be electrode-mediated. The droplet operations may redistribute and/or circulate the magnetically responsive beads within the droplet. In some cases, the droplet may include a sample droplet may include a target analyte. The redistributing of the magnetically responsive beads may cause target analyte to bind to the magnetically responsive beads. In some cases, the droplet may include unbound substances in a wash buffer. The redistributing of the magnetically responsive beads causes unbound substances to be freed from interstices of an aggregated set or subset of the magnetically responsive beads.

Abstract translation: 本发明提供了一种在液滴中重新分布磁响应珠的方法。 该方法可以包括在液滴操作表面上使用液滴进行一个或多个液滴操作，而不从磁场的区域移除磁响应珠。 在某些情况下，液滴操作可能是电极介导的。 液滴操作可以在液滴内重新分布和/或循环磁响应珠。 在一些情况下，液滴可以包括样品液滴可以包括靶分析物。 磁响应珠的重新分布可能导致目标分析物结合磁响应珠。 在一些情况下，液滴可以包括洗涤缓冲液中的未结合物质。 磁响应珠子的重新分布导致未结合物质从磁性响应珠粒的聚集或子集的空隙中释放出来。

公开(公告)号：US09023212B2

公开(公告)日：2015-05-05

申请号：US11815316

申请日：2006-01-28

Applicant: Lothar à Brassard

Inventor： Lothar à Brassard

IPC: B03C1/30 ,  B03C1/06 ,  B03C1/033 ,  B03C1/28

CPC classification number: B03C1/286 ,  B03C1/033 ,  B03C1/06 ,  B03C1/30 ,  B03C2201/18 ,  B03C2201/22 ,  B03C2201/26

Abstract: A device (10) is provided for separating magnetic or magnetizable particles from a liquid by using a magnetic field. The device includes a head piece (3) with one or more magnetizable bars (4) which is/are permanently or detachably connected with the head piece (3), as well as one or more permanent magnets (1) whose relative position with respect to the head piece can be changed by a predeterminable movement of the magnet(s) or/and by a predeterminable movement of the head piece.

Abstract translation: 提供一种用于通过使用磁场从液体中分离磁性或可磁化颗粒的装置（10）。 该装置包括具有一个或多个可磁化棒（4）的头部件（3），其可永久地或可拆卸地与头部件（3）连接，以及一个或多个永久磁铁（1），其相对位置相对于 可以通过磁体的可预定的运动或/或通过头部件的可预先确定的运动来改变头部件。

公开(公告)号：US20150059580A1

公开(公告)日：2015-03-05

申请号：US14121380

申请日：2014-08-26

Applicant: MRIGlobal

Inventor： Joseph W. Clement ,  Peter J. Anderson ,  Micah R. Decker ,  Thomas J. Keeley ,  Mary E. Pierce ,  Valorie T. Ryan ,  Darren J. Radke

IPC: B03C3/68 ,  B03C3/38

CPC classification number: B03C3/68 ,  B03C3/145 ,  B03C3/32 ,  B03C3/363 ,  B03C3/368 ,  B03C3/41 ,  B03C3/49 ,  B03C3/82 ,  B03C2201/04 ,  B03C2201/26

Abstract: A particle collector for collecting biological material using electrostatic precipitator (ESP) technology including a removable ESP collection tube having an electrode wire suspended therein, a high voltage power supply coupled to the electrode wire for generating an ionization field within the collection tube causing smaller particles entering the collection tube to precipitate onto the walls of the collection tube, electronics including a microcontroller for controlling the operation of the collector, and a housing assembly for housing the collection tube, the high voltage power supply and the electronics. Some embodiments include an interlock system for disabling high voltage to the electrode wire if certain system parameters are not met, a static discharge mechanism for dissipating excess electrostatic charge which may accumulate on the collection tube during operation, and an altitude adjustment mechanism for adjusting the amount of high voltage supplied to the electrode wire based upon the altitude at which the collector is being used. A portable hand-held embodiment of the particle collector is also disclosed.

Abstract translation: 一种用于使用静电除尘器（ESP）技术收集生物材料的颗粒收集器，其包括具有悬挂在其中的电极丝的可移除的ESP收集管，耦合到所述电极丝的高压电源，用于在所述收集管内产生电离场，导致较小的颗粒进入 所述收集管沉淀到所述收集管的壁上，所述电子器件包括用于控制所述收集器的操作的微控制器，以及用于容纳所述收集管，所述高压电源和所述电子器件的壳体组件。 一些实施例包括用于在不满足某些系统参数的情况下禁止对电极线的高电压的互锁系统，用于消除在运行期间可能积聚在收集管上的过量静电荷的静电放电机构和用于调节量的高度调节机构 基于收集器被使用的高度提供给电极线的高电压。 还公开了颗粒收集器的便携式手持实施例。

公开(公告)号：US08968542B2

公开(公告)日：2015-03-03

申请号：US12720406

申请日：2010-03-09

Applicant: Rafael V. Davalos ,  Hadi Shafiee ,  Michael B. Sano ,  John L. Caldwell

Inventor： Rafael V. Davalos ,  Hadi Shafiee ,  Michael B. Sano ,  John L. Caldwell

IPC: B01D57/02 ,  B03C5/02 ,  B03C5/00

CPC classification number: G01N27/44791 ,  B01D57/02 ,  B03C5/005 ,  B03C5/022 ,  B03C5/026 ,  B03C2201/26

Abstract: Devices and methods for performing dielectrophoresis are described. The devices contain sample channel which is separated by physical barriers from electrode channels which receive electrodes. The devices and methods may be used for the separation and analysis of particles in solution, including the separation and isolation of cells of a specific type. As the electrodes do not make contact with the sample, electrode fouling is avoided and sample integrity is better maintained.

Abstract translation: 描述了用于进行介电电泳的装置和方法。 这些器件包含由接收电极的电极通道的物理屏障分离的样品通道。 该装置和方法可用于分离和分析溶液中的颗粒，包括分离和分离特定类型的细胞。 由于电极不与样品接触，避免了电极结垢，更好地保持了样品的完整性。

公开(公告)号：US20150041325A1

公开(公告)日：2015-02-12

申请号：US14388212

申请日：2013-03-27

Applicant: The Regents Of The University of California

Inventor： Pei-yu E. Chiou ,  Kuo-wei Huang ,  Yu-jui Fan ,  Yu-chun Kung

IPC: B03C5/00

CPC classification number: B03C5/005 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0424 ,  B01L2400/0655 ,  B03C5/026 ,  B03C2201/26 ,  G01N15/0656 ,  G01N27/44778 ,  G01N2015/0065 ,  G01N2015/1081

Abstract: A 3-dimensional PDMS cell sorter having multiple passages in a PDMS layer that follow the same path in a DEP separation region and that are in fluid communication with each other within that region. The passages may differ in width transverse to the flow direction within the passages. Flat plates may sandwich the PDMS layer; each plate may have a planar electrode used to generate a DEP field within a sample fluid flowed within the passages. The DEP field may concentrate target cells or particulates within one of the passages within the DEP separation region. The passages may diverge after the DEP-separation region, leaving one passage with a high concentration of target cells or particulates. Techniques for manufacturing such structures, as well as other micro-fluidic structures, are also provided.

Abstract translation: 三维PDMS细胞分选器在PDMS层中具有沿DEP分离区域中相同路径并且在该区域内彼此流体连通的多个通道。 这些通道在通道内横向于流动方向的宽度可能不同。 平板可夹住PDMS层; 每个板可以具有用于在通道内流动的样品流体内产生DEP场的平面电极。 DEP场可以将目标细胞或微粒集中在DEP分离区域内的通道之一内。 通道在DEP分离区域之后可能发生分离，留下一个具有高浓度靶细胞或微粒的通道。 还提供了用于制造这种结构以及其它微流体结构的技术。

公开(公告)号：US20150014171A1

公开(公告)日：2015-01-15

申请号：US14264141

申请日：2014-04-29

Applicant: National Taiwan University

Inventor： Shiang-Chi LIN ,  Chih-Ting LIN ,  Yi-Chung TUNG ,  Yu-Lung SUNG

IPC: B03C5/00 ,  B03C5/02

CPC classification number: B03C5/005 ,  B03C5/028 ,  B03C2201/26

Abstract: A microfluidic particle separation device includes a substrate and a plurality of electrode bars formed on the substrate, disposed around as array center, angularly spaced apart from one another, and extending radially with respect to the array center so as to form a radially-extending electrode array that is capable of inducing circular or elliptical shear flow of the liquid through travelling-wave electroosmosis when being applied with a travelling-wave electric potential.

Abstract translation: 微流体颗粒分离装置包括基板和形成在基板上的多个电极棒，其设置为围绕阵列中心彼此成角度地间隔开并且相对于阵列中心径向延伸，以形成径向延伸的电极 阵列，当施加行波电位时能够通过行波电渗引起液体的圆形或椭圆形剪切流动。

公开(公告)号：US08932815B2

公开(公告)日：2015-01-13

申请号：US14311037

申请日：2014-06-20

Applicant: Biological Dynamics, Inc.

Inventor： Rajaram Krishnan ,  David J. Charlot ,  Eugene Tu ,  James McCanna ,  Lucas Kumosa ,  Paul D. Swanson ,  Robert Turner ,  Kai Yang ,  Irina Dobrovolskaya ,  David Liu ,  Juan Pablo Hinestrosa Salazar ,  Juscilene Menezes

IPC: C12Q1/68 ,  C12N13/00 ,  G01N27/447

CPC classification number: C12Q1/6825 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/18 ,  B03C2201/26 ,  C12N15/1003 ,  C12N15/101 ,  C12Q1/6806 ,  C12Q1/6869 ,  G01N27/447

Abstract: The present invention includes methods, devices and systems for isolating a nucleic acid from a fluid comprising cells. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity nucleic acid isolated from complex fluids such as blood or environmental samples.