公开(公告)号：US5120662A

公开(公告)日：1992-06-09

申请号：US532489

申请日：1990-06-04

Applicant: Emerson W. Chan ,  Werner Schulze ,  William G. Robey ,  Brian P. Braun ,  Cynthia K. Daluga ,  Andreas A. Kapsalis ,  Kevin M. Knigge ,  John E. Stephens ,  Joseph J. Stojak, II ,  David S. Vallaris ,  Benton A. Durley, deceased ,  James D. Defreese ,  Carl W. Merkh

Inventor： Emerson W. Chan ,  Werner Schulze ,  William G. Robey ,  Brian P. Braun ,  Cynthia K. Daluga ,  Andreas A. Kapsalis ,  Kevin M. Knigge ,  John E. Stephens ,  Joseph J. Stojak, II ,  David S. Vallaris ,  Benton A. Durley, deceased ,  James D. Defreese ,  Carl W. Merkh

IPC: G01N33/543 ,  G01N33/569

CPC classification number: G01N33/54306 ,  G01N33/56988 ,  Y10S436/808 ,  Y10S436/809

Abstract: An immunoassay which is capable of simultaneously detecting any desired number of antigens of one infectious agent, or combinations of antigens of several infectious agents, or any desired number of immunoglobulins of one infectious agent, or combinations of immunoglobulins of several infectious agents on a single solid support. A test sample is contacted with a solid support on which one or more antigens are immobilized as discrete test sites. Antigen-antibody complexes are formed and detected on the solid support.

Abstract translation: 能够同时检测一种感染因子的任何所需数量的抗原或几种感染因子的抗原的组合或一种感染因子的任何所需数量的免疫球蛋白或几种感染因子的免疫球蛋白的组合在单一固体上的免疫测定 支持。 将测试样品与其上固定有一种或多种抗原的固体支持物作为离散测试位点接触。 在固体支持物上形成并检测抗原 - 抗体复合物。

公开(公告)号：US5281540A

公开(公告)日：1994-01-25

申请号：US796942

申请日：1991-11-22

Applicant: Carl W. Merkh ,  James D. Defreese ,  Benton A. Durley, III, deceased

Inventor： Carl W. Merkh ,  James D. Defreese ,  Benton A. Durley, III, deceased

IPC: G01N33/50 ,  B01L3/00 ,  G01N33/543 ,  G01N33/68 ,  G01N35/00 ,  G01N35/10 ,  G01N33/545 ,  G01N33/544

CPC classification number: B01L3/545 ,  G01N33/54366 ,  G01N33/6854 ,  G01N35/00029 ,  G01N2021/478 ,  G01N2035/00099 ,  G01N2035/00752 ,  G01N2035/1025 ,  G01N2201/0415 ,  G01N2201/0621 ,  G01N2201/1211

Abstract: A semi-automated biological sample analyzer and subsystems are provided to simultaneously perform a plurality of enzyme immunoassays for human IgE class antibodies specific to a panel of preselected allergens in each of a plurality of biological samples. A carousel is provided to position and hold a plurality of reaction cartridges. Each reaction cartridge includes a plurality of isolated test sites formed in a two dimensional array in a solid phase binding layer contained within a reaction well which is adapted to contain a biological sample to be assayed. The carousel and cartridges contain structures which cooperate to precisely position the cartridges in each of three separate dimensions so that each cartridge is positioned uniformly. An optical reader operating on a principle of diffuse reflectance is provided to read the results of the assays from each test site of each cartridge. Also provided is a subsystem which provides predetermined lot-specific assay calibration data which is useful for normalizing the results of various assays with respect to predetermined common standard values.

Abstract translation: 提供半自动生物样品分析仪和子系统以同时对多个生物样品中的每一个中预先选择的过敏原组特异的人IgE类抗体进行多次酶免疫测定。 提供转盘以定位和保持多个反应盒。 每个反应盒包括在包含在反应井内的固相结合层中的二维阵列中形成的多个分离的测试位点，其适于含有要测定的生物样品。 圆盘传送带和墨盒包含结构，其配合以将墨盒精确地定位在三个分开的尺寸中的每一个中，使得每个墨盒均匀地定位。 提供以漫反射原理工作的光学阅读器，以从每个盒的每个测试位置读取测定结果。 还提供了一种子系统，其提供预定的特异性测定校准数据，其可用于相对于预定的共同标准值标准化各种测定的结果。

公开(公告)号：US5075077A

公开(公告)日：1991-12-24

申请号：US227272

申请日：1988-08-02

Applicant: Benton A. Durley, III ,  James D. Defreese ,  Carl W. Merkh

Inventor： Benton A. Durley, III ,  James D. Defreese ,  Carl W. Merkh

IPC: B01L3/00 ,  G01N33/543 ,  G01N33/68 ,  G01N35/00 ,  G01N35/10

CPC classification number: G01N35/00029 ,  B01L3/545 ,  G01N33/54366 ,  G01N33/6854 ,  G01N2021/478 ,  G01N2035/00099 ,  G01N2035/00752 ,  G01N2035/1025 ,  G01N2201/0415 ,  G01N2201/0621 ,  G01N2201/1211 ,  Y10S435/805

Abstract: A semi-automated biological sample analyzer and subsystems are provided to simultaneously perform a plurality of enzyme immuno assays for human IgE class antibodies specific to a panel of preselected allergens in each of a plurality of biological samples. A carousel is provided to position and hold a plurality of reaction cartridges. Each reaction cartridge includes a plurality of isolated test sites formed in a two dimensional array in a solid phase binding layer contained within a reaction well which is adapted to contain a biological sample to be assayed. The carousel and cartridges contain structures which cooperate to precisely position the cartridges in each of three separate dimensions so that each cartridge is positioned uniformly. An optical reader operating on a principle of diffuse reflectance is provided to read the results of the assays from each test site of each cartridge. Also provided is a subsystem which provides predetermined lot-specific assay calibration data which is useful for normalizing the results of various assays with respect to predetermined common standard values.