公开(公告)号：US20180136110A1

公开(公告)日：2018-05-17

申请号：US15801055

申请日：2017-11-01

Applicant: Abbott Laboratories

Inventor： Jiong Wu ,  Michael R. Buhl ,  Giacomo Vacca

IPC: G01N15/14 ,  G01N21/64 ,  G01N33/49 ,  G01N15/00 ,  G01N15/10

Abstract: Provided herein are systems and methods for analyzing blood samples, and more specifically for performing a basophil analysis. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; and then (b) using measurements of light scatter and fluorescence emission to distinguish basophils from other WBC sub-populations. In one embodiment, the systems and methods include performing a basophil cluster analysis of the blood sample, based on the combination of light scatter and fluorescence measurements.

公开(公告)号：US20160018312A1

公开(公告)日：2016-01-21

申请号：US14808720

申请日：2015-07-24

Applicant: Abbott Laboratories

Inventor： Jiong Wu ,  Marilou Coleman ,  Emily H. Lin ,  Michael R. Buhl ,  Giacomo Vacca

IPC: G01N15/14 ,  G01N21/64 ,  G01N33/49

CPC classification number: G01N15/1434 ,  G01N15/1459 ,  G01N15/147 ,  G01N21/6428 ,  G01N33/49 ,  G01N33/80 ,  G01N2015/0069 ,  G01N2015/008 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2015/1477 ,  G01N2015/1488 ,  G01N2021/6439

Abstract: Systems and methods for analyzing blood samples, and more specifically for performing a nucleated red blood cell (nRBC) analysis. The systems and methods screen a blood sample by means of fluorescence staining and a fluorescence triggering strategy, to identify nuclei-containing particles within the blood sample. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder reagent(s), suitable for assays of samples containing fragile white blood cells (WBCs). In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; (b) using a fluorescence trigger to screen the blood sample for nuclei-containing particles; and (c) using measurements of light scatter and fluorescence emission to distinguish nRBCs from WBCs.

Abstract translation: 用于分析血液样品的系统和方法，更具体地用于进行有核红细胞（nRBC）分析。 系统和方法通过荧光染色和荧光触发策略筛选血液样品，以鉴定血液样品中含有核的颗粒。 因此，基本上消除了来自未分解的红细胞（RBC）和裂解的RBC的片段的干扰。 这些系统和方法还能够开发适合于含有易碎白细胞（WBC）的样品的测定的较温和的试剂。 在一个实施方案中，系统和方法包括：（a）用专用细胞膜可渗透荧光染料染色血液样品; （b）使用荧光触发器来筛选含有核的颗粒的血液样品; 和（c）使用光散射和荧光发射的测量来区分来自WBC的nRBC。

公开(公告)号：US10481072B2

公开(公告)日：2019-11-19

申请号：US15704974

申请日：2017-09-14

Applicant: Abbott Laboratories

Inventor： Jiong Wu ,  Marilou Coleman ,  Emily H. Lin ,  Michael R. Buhl ,  Giacomo Vacca

IPC: G01N15/14 ,  G01N33/80 ,  G01N21/64 ,  G01N33/49 ,  G01N15/10 ,  G01N15/00

Abstract: Systems and methods for analyzing blood samples, and more specifically for performing a nucleated red blood cell (nRBC) analysis. The systems and methods screen a blood sample by means of fluorescence staining and a fluorescence triggering strategy, to identify nuclei-containing particles within the blood sample. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder reagent(s), suitable for assays of samples containing fragile white blood cells (WBCs). In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; (b) using a fluorescence trigger to screen the blood sample for nuclei-containing particles; and (c) using measurements of light scatter and fluorescence emission to distinguish nRBCs from WBCs.

公开(公告)号：US20180128732A1

公开(公告)日：2018-05-10

申请号：US15704974

申请日：2017-09-14

Applicant: Abbott Laboratories

Inventor： Jiong Wu ,  Marilou Coleman ,  Emily H. Lin ,  Michael R. Buhl ,  Giacomo Vacca

IPC: G01N15/14 ,  G01N21/64 ,  G01N33/49 ,  G01N33/80 ,  G01N15/00 ,  G01N15/10

CPC classification number: G01N15/1434 ,  G01N15/1459 ,  G01N15/147 ,  G01N21/6428 ,  G01N33/49 ,  G01N33/80 ,  G01N2015/0069 ,  G01N2015/008 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2015/1477 ,  G01N2015/1488 ,  G01N2021/6439

Abstract: Systems and methods for analyzing blood samples, and more specifically for performing a nucleated red blood cell (nRBC) analysis. The systems and methods screen a blood sample by means of fluorescence staining and a fluorescence triggering strategy, to identify nuclei-containing particles within the blood sample. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder reagent(s), suitable for assays of samples containing fragile white blood cells (WBCs). In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; (b) using a fluorescence trigger to screen the blood sample for nuclei-containing particles; and (c) using measurements of light scatter and fluorescence emission to distinguish nRBCs from WBCs.

公开(公告)号：US09810618B2

公开(公告)日：2017-11-07

申请号：US14808211

申请日：2015-07-24

Applicant: Abbott Laboratories

Inventor： Jiong Wu ,  Michael R. Buhl ,  Giacomo Vacca

IPC: G01N15/14 ,  G01N21/64 ,  G01N33/49 ,  G01N15/00 ,  G01N15/10

CPC classification number: G01N15/1434 ,  G01N15/1459 ,  G01N21/6428 ,  G01N33/49 ,  G01N2015/0069 ,  G01N2015/008 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2015/1477 ,  G01N2015/1486 ,  G01N2015/1488 ,  G01N2021/6439

Abstract: Provided herein are systems and methods for analyzing blood samples, and more specifically for performing a basophil analysis. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; and then (b) using measurements of light scatter and fluorescence emission to distinguish basophils from other WBC sub-populations. In one embodiment, the systems and methods include performing a basophil cluster analysis of the blood sample, based on the combination of light scatter and fluorescence measurements.

公开(公告)号：US10481073B2

公开(公告)日：2019-11-19

申请号：US15801055

申请日：2017-11-01

Applicant: Abbott Laboratories

Inventor： Jiong Wu ,  Michael R. Buhl ,  Giacomo Vacca

IPC: G01N15/14 ,  G01N21/64 ,  G01N33/49 ,  G01N15/10 ,  G01N15/00

Abstract: Provided herein are systems and methods for analyzing blood samples, and more specifically for performing a basophil analysis. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; and then (b) using measurements of light scatter and fluorescence emission to distinguish basophils from other WBC sub-populations. In one embodiment, the systems and methods include performing a basophil cluster analysis of the blood sample, based on the combination of light scatter and fluorescence measurements.

公开(公告)号：US09778163B2

公开(公告)日：2017-10-03

申请号：US14808720

申请日：2015-07-24

Applicant: Abbott Laboratories

Inventor： Jiong Wu ,  Marilou Coleman ,  Emily H. Lin ,  Michael R. Buhl ,  Giacomo Vacca

IPC: G01N15/14 ,  G01N33/49 ,  G01N21/64 ,  G01N33/80 ,  G01N15/00 ,  G01N15/10

CPC classification number: G01N15/1434 ,  G01N15/1459 ,  G01N15/147 ,  G01N21/6428 ,  G01N33/49 ,  G01N33/80 ,  G01N2015/0069 ,  G01N2015/008 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2015/1477 ,  G01N2015/1488 ,  G01N2021/6439

Abstract: Systems and methods for analyzing blood samples, and more specifically for performing a nucleated red blood cell (nRBC) analysis. The systems and methods screen a blood sample by means of fluorescence staining and a fluorescence triggering strategy, to identify nuclei-containing particles within the blood sample. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder reagent(s), suitable for assays of samples containing fragile white blood cells (WBCs). In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; (b) using a fluorescence trigger to screen the blood sample for nuclei-containing particles; and (c) using measurements of light scatter and fluorescence emission to distinguish nRBCs from WBCs.

公开(公告)号：US20160018311A1

公开(公告)日：2016-01-21

申请号：US14808211

申请日：2015-07-24

Applicant: Abbott Laboratories

Inventor： Jiong Wu ,  Michael R. Buhl ,  Giacomo Vacca

IPC: G01N15/14 ,  G01N33/49 ,  G01N21/64

CPC classification number: G01N15/1434 ,  G01N15/1459 ,  G01N21/6428 ,  G01N33/49 ,  G01N2015/0069 ,  G01N2015/008 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2015/1477 ,  G01N2015/1486 ,  G01N2015/1488 ,  G01N2021/6439

Abstract: Provided herein are systems and methods for analyzing blood samples, and more specifically for performing a basophil analysis. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; and then (b) using measurements of light scatter and fluorescence emission to distinguish basophils from other WBC sub-populations. In one embodiment, the systems and methods include performing a basophil cluster analysis of the blood sample, based on the combination of light scatter and fluorescence measurements.

Abstract translation: 本文提供了用于分析血液样品的系统和方法，并且更具体地用于进行嗜碱性粒细胞分析。 在一个实施方案中，系统和方法包括：（a）用专用细胞膜可渗透荧光染料染色血液样品; 然后（b）使用光散射和荧光发射的测量来区分嗜碱性粒细胞与其他WBC亚种群。 在一个实施方案中，系统和方法包括基于光散射和荧光测量的组合进行血液样品的嗜碱性粒细胞聚类分析。