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公开(公告)号：US20180136110A1
公开(公告)日：2018-05-17
申请号：US15801055
申请日：2017-11-01
Applicant: Abbott Laboratories
Inventor： Jiong Wu , Michael R. Buhl , Giacomo Vacca
Abstract: Provided herein are systems and methods for analyzing blood samples, and more specifically for performing a basophil analysis. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; and then (b) using measurements of light scatter and fluorescence emission to distinguish basophils from other WBC sub-populations. In one embodiment, the systems and methods include performing a basophil cluster analysis of the blood sample, based on the combination of light scatter and fluorescence measurements.
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公开(公告)号：US20180136109A1
公开(公告)日：2018-05-17
申请号：US15786899
申请日：2017-10-18
Applicant: Abbott Laboratories
Inventor： Jiong Wu , Giacomo Vacca
CPC classification number: G01N15/1434 , G01N15/147 , G01N21/6428 , G01N33/5047 , G01N33/5094 , G01N33/582 , G01N35/1002 , G01N2015/0065 , G01N2015/0069 , G01N2015/1006 , G01N2015/1486 , G01N2015/149 , G01N2021/6439
Abstract: A method whereby one or more fluorescent dyes are used to bind and stain nucleic acids in certain blood cells, such as, for example, white blood cells, nucleated red blood cells, and reticulocytes, and to induce fluorescent emissions upon excitation of photons from a given source of light, such as, for example, a laser, at an appropriate wavelength. More particularly, this invention provides a method whereby a fluorescent trigger is used in a data collection step for collecting events that emit strong fluorescence, in order to separate white blood cells and nucleated red blood cells from red blood cells and platelets without the need for using a lysing agent.
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公开(公告)号：US09797824B2
公开(公告)日：2017-10-24
申请号：US14808725
申请日：2015-07-24
Applicant: Abbott Laboratories
Inventor： Jiong Wu , Giacomo Vacca
CPC classification number: G01N15/1434 , G01N15/147 , G01N21/6428 , G01N33/5047 , G01N33/5094 , G01N33/582 , G01N35/1002 , G01N2015/0065 , G01N2015/0069 , G01N2015/1006 , G01N2015/1486 , G01N2015/149 , G01N2021/6439
Abstract: A method whereby one or more fluorescent dyes are used to bind and stain nucleic acids in certain blood cells, such as, for example, white blood cells, nucleated red blood cells, and reticulocytes, and to induce fluorescent emissions upon excitation of photons from a given source of light, such as, for example, a laser, at an appropriate wavelength. More particularly, this invention provides a method whereby a fluorescent trigger is used in a data collection step for collecting events that emit strong fluorescence, in order to separate white blood cells and nucleated red blood cells from red blood cells and platelets without the need for using a lysing agent.
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公开(公告)号：US09778162B2
公开(公告)日：2017-10-03
申请号：US14808205
申请日：2015-07-24
Applicant: Abbott Laboratories
Inventor： Jiong Wu , Giacomo Vacca
CPC classification number: G01N15/1434 , G01N15/1459 , G01N21/6428 , G01N33/49 , G01N2015/008 , G01N2015/1006 , G01N2015/1477 , G01N2015/1488 , G01N2021/6439
Abstract: Systems and methods for analyzing blood samples, and more specifically for performing a white blood cell (WBC) differential analysis. The systems and methods screen WBCs by means of fluorescence staining and a fluorescence triggering strategy. As such, interference from unlyzed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder WBC reagent(s), suitable for assays of samples containing fragile WBCs. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye, which corresponds in emission spectrum to an excitation source of a hematology instrument; (b) using a fluorescence trigger to screen the blood sample for WBCs; and (c) using measurements of (1) axial light loss, (2) intermediate angle scatter, (3) 90° polarized side scatter, (4) 90° depolarized side scatter, and (5) fluorescence emission to perform a differentiation analysis.
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公开(公告)号：US20160258857A1
公开(公告)日：2016-09-08
申请号：US15044636
申请日：2016-02-16
Applicant: Abbott Laboratories
Inventor： Martin Krockenberger , Jiong Wu , Bodo Roemer , Giacomo Vacca
CPC classification number: G01N15/1404 , G01N15/00 , G01N15/1429 , G01N15/1459 , G01N21/53 , G01N33/49 , G01N33/4915 , G01N33/492 , G01N2015/0073 , G01N2015/0076 , G01N2015/008 , G01N2015/0084 , G01N2015/1006 , G01N2015/1486 , G01N2800/22 , Y10T436/101666
Abstract: A method for identifying, analyzing, and quantifying the cellular components of whole blood by means of an automated hematology analyzer and the detection of the light scattered, absorbed, and fluorescently emitted by each cell. More particularly, the aforementioned method involves identifying, analyzing, and quantifying the cellular components of whole blood by means of a light source having a wavelength ranging from about 400 nm to about 450 nm and multiple in-flow optical measurements and staining without the need for lysing red blood cells.
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Patent Agency Ranking
公开(公告)号：US20160018312A1
公开(公告)日：2016-01-21
申请号：US14808720
申请日：2015-07-24
Applicant: Abbott Laboratories
Inventor： Jiong Wu , Marilou Coleman , Emily H. Lin , Michael R. Buhl , Giacomo Vacca
CPC classification number: G01N15/1434 , G01N15/1459 , G01N15/147 , G01N21/6428 , G01N33/49 , G01N33/80 , G01N2015/0069 , G01N2015/008 , G01N2015/1006 , G01N2015/1402 , G01N2015/1477 , G01N2015/1488 , G01N2021/6439
Abstract: Systems and methods for analyzing blood samples, and more specifically for performing a nucleated red blood cell (nRBC) analysis. The systems and methods screen a blood sample by means of fluorescence staining and a fluorescence triggering strategy, to identify nuclei-containing particles within the blood sample. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder reagent(s), suitable for assays of samples containing fragile white blood cells (WBCs). In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; (b) using a fluorescence trigger to screen the blood sample for nuclei-containing particles; and (c) using measurements of light scatter and fluorescence emission to distinguish nRBCs from WBCs.
Abstract translation: 用于分析血液样品的系统和方法，更具体地用于进行有核红细胞（nRBC）分析。 系统和方法通过荧光染色和荧光触发策略筛选血液样品，以鉴定血液样品中含有核的颗粒。 因此，基本上消除了来自未分解的红细胞（RBC）和裂解的RBC的片段的干扰。 这些系统和方法还能够开发适合于含有易碎白细胞（WBC）的样品的测定的较温和的试剂。 在一个实施方案中，系统和方法包括：（a）用专用细胞膜可渗透荧光染料染色血液样品; （b）使用荧光触发器来筛选含有核的颗粒的血液样品; 和（c）使用光散射和荧光发射的测量来区分来自WBC的nRBC。
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公开(公告)号：US10481073B2
公开(公告)日：2019-11-19
申请号：US15801055
申请日：2017-11-01
Applicant: Abbott Laboratories
Inventor： Jiong Wu , Michael R. Buhl , Giacomo Vacca
Abstract: Provided herein are systems and methods for analyzing blood samples, and more specifically for performing a basophil analysis. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; and then (b) using measurements of light scatter and fluorescence emission to distinguish basophils from other WBC sub-populations. In one embodiment, the systems and methods include performing a basophil cluster analysis of the blood sample, based on the combination of light scatter and fluorescence measurements.
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公开(公告)号：US09778163B2
公开(公告)日：2017-10-03
申请号：US14808720
申请日：2015-07-24
Applicant: Abbott Laboratories
Inventor： Jiong Wu , Marilou Coleman , Emily H. Lin , Michael R. Buhl , Giacomo Vacca
CPC classification number: G01N15/1434 , G01N15/1459 , G01N15/147 , G01N21/6428 , G01N33/49 , G01N33/80 , G01N2015/0069 , G01N2015/008 , G01N2015/1006 , G01N2015/1402 , G01N2015/1477 , G01N2015/1488 , G01N2021/6439
Abstract: Systems and methods for analyzing blood samples, and more specifically for performing a nucleated red blood cell (nRBC) analysis. The systems and methods screen a blood sample by means of fluorescence staining and a fluorescence triggering strategy, to identify nuclei-containing particles within the blood sample. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder reagent(s), suitable for assays of samples containing fragile white blood cells (WBCs). In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; (b) using a fluorescence trigger to screen the blood sample for nuclei-containing particles; and (c) using measurements of light scatter and fluorescence emission to distinguish nRBCs from WBCs.
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公开(公告)号：US20160018311A1
公开(公告)日：2016-01-21
申请号：US14808211
申请日：2015-07-24
Applicant: Abbott Laboratories
Inventor： Jiong Wu , Michael R. Buhl , Giacomo Vacca
CPC classification number: G01N15/1434 , G01N15/1459 , G01N21/6428 , G01N33/49 , G01N2015/0069 , G01N2015/008 , G01N2015/1006 , G01N2015/1402 , G01N2015/1477 , G01N2015/1486 , G01N2015/1488 , G01N2021/6439
Abstract: Provided herein are systems and methods for analyzing blood samples, and more specifically for performing a basophil analysis. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; and then (b) using measurements of light scatter and fluorescence emission to distinguish basophils from other WBC sub-populations. In one embodiment, the systems and methods include performing a basophil cluster analysis of the blood sample, based on the combination of light scatter and fluorescence measurements.
Abstract translation: 本文提供了用于分析血液样品的系统和方法，并且更具体地用于进行嗜碱性粒细胞分析。 在一个实施方案中，系统和方法包括：（a）用专用细胞膜可渗透荧光染料染色血液样品; 然后（b）使用光散射和荧光发射的测量来区分嗜碱性粒细胞与其他WBC亚种群。 在一个实施方案中，系统和方法包括基于光散射和荧光测量的组合进行血液样品的嗜碱性粒细胞聚类分析。
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公开(公告)号：US11378578B2
公开(公告)日：2022-07-05
申请号：US14137392
申请日：2013-12-20
Applicant: Abbott Laboratories
Abstract: Aspects of the invention include hematology analysis reagents, systems and methods that can be used to preserve blood cell morphology and integrity as well as provide sample integrity and optical clarity to facilitate optical analysis of blood samples. In some embodiments, the reagents include a non-phosphate organic buffer and a sphering surfactant. The pH and osmolality of the reagents may be adjusted to desired ranges. In addition, the reagents can be simply diluted with de-ionized water prior to use.
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