公开(公告)号：US10481073B2

公开(公告)日：2019-11-19

申请号：US15801055

申请日：2017-11-01

Applicant: Abbott Laboratories

Inventor： Jiong Wu ,  Michael R. Buhl ,  Giacomo Vacca

IPC: G01N15/14 ,  G01N21/64 ,  G01N33/49 ,  G01N15/10 ,  G01N15/00

Abstract: Provided herein are systems and methods for analyzing blood samples, and more specifically for performing a basophil analysis. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; and then (b) using measurements of light scatter and fluorescence emission to distinguish basophils from other WBC sub-populations. In one embodiment, the systems and methods include performing a basophil cluster analysis of the blood sample, based on the combination of light scatter and fluorescence measurements.

公开(公告)号：US09778163B2

公开(公告)日：2017-10-03

申请号：US14808720

申请日：2015-07-24

Applicant: Abbott Laboratories

Inventor： Jiong Wu ,  Marilou Coleman ,  Emily H. Lin ,  Michael R. Buhl ,  Giacomo Vacca

IPC: G01N15/14 ,  G01N33/49 ,  G01N21/64 ,  G01N33/80 ,  G01N15/00 ,  G01N15/10

CPC classification number: G01N15/1434 ,  G01N15/1459 ,  G01N15/147 ,  G01N21/6428 ,  G01N33/49 ,  G01N33/80 ,  G01N2015/0069 ,  G01N2015/008 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2015/1477 ,  G01N2015/1488 ,  G01N2021/6439

Abstract: Systems and methods for analyzing blood samples, and more specifically for performing a nucleated red blood cell (nRBC) analysis. The systems and methods screen a blood sample by means of fluorescence staining and a fluorescence triggering strategy, to identify nuclei-containing particles within the blood sample. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder reagent(s), suitable for assays of samples containing fragile white blood cells (WBCs). In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; (b) using a fluorescence trigger to screen the blood sample for nuclei-containing particles; and (c) using measurements of light scatter and fluorescence emission to distinguish nRBCs from WBCs.

公开(公告)号：US20160018311A1

公开(公告)日：2016-01-21

申请号：US14808211

申请日：2015-07-24

Applicant: Abbott Laboratories

Inventor： Jiong Wu ,  Michael R. Buhl ,  Giacomo Vacca

IPC: G01N15/14 ,  G01N33/49 ,  G01N21/64

CPC classification number: G01N15/1434 ,  G01N15/1459 ,  G01N21/6428 ,  G01N33/49 ,  G01N2015/0069 ,  G01N2015/008 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2015/1477 ,  G01N2015/1486 ,  G01N2015/1488 ,  G01N2021/6439

Abstract: Provided herein are systems and methods for analyzing blood samples, and more specifically for performing a basophil analysis. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; and then (b) using measurements of light scatter and fluorescence emission to distinguish basophils from other WBC sub-populations. In one embodiment, the systems and methods include performing a basophil cluster analysis of the blood sample, based on the combination of light scatter and fluorescence measurements.

Abstract translation: 本文提供了用于分析血液样品的系统和方法，并且更具体地用于进行嗜碱性粒细胞分析。 在一个实施方案中，系统和方法包括：（a）用专用细胞膜可渗透荧光染料染色血液样品; 然后（b）使用光散射和荧光发射的测量来区分嗜碱性粒细胞与其他WBC亚种群。 在一个实施方案中，系统和方法包括基于光散射和荧光测量的组合进行血液样品的嗜碱性粒细胞聚类分析。

公开(公告)号：US20180136110A1

公开(公告)日：2018-05-17

申请号：US15801055

申请日：2017-11-01

Applicant: Abbott Laboratories

Inventor： Jiong Wu ,  Michael R. Buhl ,  Giacomo Vacca

IPC: G01N15/14 ,  G01N21/64 ,  G01N33/49 ,  G01N15/00 ,  G01N15/10

Abstract: Provided herein are systems and methods for analyzing blood samples, and more specifically for performing a basophil analysis. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; and then (b) using measurements of light scatter and fluorescence emission to distinguish basophils from other WBC sub-populations. In one embodiment, the systems and methods include performing a basophil cluster analysis of the blood sample, based on the combination of light scatter and fluorescence measurements.

公开(公告)号：US20180136109A1

公开(公告)日：2018-05-17

申请号：US15786899

申请日：2017-10-18

Applicant: Abbott Laboratories

Inventor： Jiong Wu ,  Giacomo Vacca

IPC: G01N15/14 ,  G01N21/64 ,  G01N33/50 ,  G01N33/58 ,  G01N35/10 ,  G01N15/00 ,  G01N15/10

CPC classification number: G01N15/1434 ,  G01N15/147 ,  G01N21/6428 ,  G01N33/5047 ,  G01N33/5094 ,  G01N33/582 ,  G01N35/1002 ,  G01N2015/0065 ,  G01N2015/0069 ,  G01N2015/1006 ,  G01N2015/1486 ,  G01N2015/149 ,  G01N2021/6439

Abstract: A method whereby one or more fluorescent dyes are used to bind and stain nucleic acids in certain blood cells, such as, for example, white blood cells, nucleated red blood cells, and reticulocytes, and to induce fluorescent emissions upon excitation of photons from a given source of light, such as, for example, a laser, at an appropriate wavelength. More particularly, this invention provides a method whereby a fluorescent trigger is used in a data collection step for collecting events that emit strong fluorescence, in order to separate white blood cells and nucleated red blood cells from red blood cells and platelets without the need for using a lysing agent.

公开(公告)号：US09797824B2

公开(公告)日：2017-10-24

申请号：US14808725

申请日：2015-07-24

Applicant: Abbott Laboratories

Inventor： Jiong Wu ,  Giacomo Vacca

IPC: G01N15/14 ,  G01N21/64 ,  G01N35/10 ,  G01N33/50 ,  G01N33/58 ,  G01N15/10 ,  G01N15/00

CPC classification number: G01N15/1434 ,  G01N15/147 ,  G01N21/6428 ,  G01N33/5047 ,  G01N33/5094 ,  G01N33/582 ,  G01N35/1002 ,  G01N2015/0065 ,  G01N2015/0069 ,  G01N2015/1006 ,  G01N2015/1486 ,  G01N2015/149 ,  G01N2021/6439

Abstract: A method whereby one or more fluorescent dyes are used to bind and stain nucleic acids in certain blood cells, such as, for example, white blood cells, nucleated red blood cells, and reticulocytes, and to induce fluorescent emissions upon excitation of photons from a given source of light, such as, for example, a laser, at an appropriate wavelength. More particularly, this invention provides a method whereby a fluorescent trigger is used in a data collection step for collecting events that emit strong fluorescence, in order to separate white blood cells and nucleated red blood cells from red blood cells and platelets without the need for using a lysing agent.

公开(公告)号：US09778162B2

公开(公告)日：2017-10-03

申请号：US14808205

申请日：2015-07-24

Applicant: Abbott Laboratories

Inventor： Jiong Wu ,  Giacomo Vacca

IPC: G01N15/14 ,  G01N21/64 ,  G01N15/10 ,  G01N15/08 ,  G01N33/49 ,  G01N15/00

CPC classification number: G01N15/1434 ,  G01N15/1459 ,  G01N21/6428 ,  G01N33/49 ,  G01N2015/008 ,  G01N2015/1006 ,  G01N2015/1477 ,  G01N2015/1488 ,  G01N2021/6439

Abstract: Systems and methods for analyzing blood samples, and more specifically for performing a white blood cell (WBC) differential analysis. The systems and methods screen WBCs by means of fluorescence staining and a fluorescence triggering strategy. As such, interference from unlyzed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder WBC reagent(s), suitable for assays of samples containing fragile WBCs. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye, which corresponds in emission spectrum to an excitation source of a hematology instrument; (b) using a fluorescence trigger to screen the blood sample for WBCs; and (c) using measurements of (1) axial light loss, (2) intermediate angle scatter, (3) 90° polarized side scatter, (4) 90° depolarized side scatter, and (5) fluorescence emission to perform a differentiation analysis.

公开(公告)号：US20160258857A1

公开(公告)日：2016-09-08

申请号：US15044636

申请日：2016-02-16

Applicant: Abbott Laboratories

Inventor： Martin Krockenberger ,  Jiong Wu ,  Bodo Roemer ,  Giacomo Vacca

IPC: G01N15/14 ,  G01N33/49

CPC classification number: G01N15/1404 ,  G01N15/00 ,  G01N15/1429 ,  G01N15/1459 ,  G01N21/53 ,  G01N33/49 ,  G01N33/4915 ,  G01N33/492 ,  G01N2015/0073 ,  G01N2015/0076 ,  G01N2015/008 ,  G01N2015/0084 ,  G01N2015/1006 ,  G01N2015/1486 ,  G01N2800/22 ,  Y10T436/101666

Abstract: A method for identifying, analyzing, and quantifying the cellular components of whole blood by means of an automated hematology analyzer and the detection of the light scattered, absorbed, and fluorescently emitted by each cell. More particularly, the aforementioned method involves identifying, analyzing, and quantifying the cellular components of whole blood by means of a light source having a wavelength ranging from about 400 nm to about 450 nm and multiple in-flow optical measurements and staining without the need for lysing red blood cells.

公开(公告)号：US09261495B2

公开(公告)日：2016-02-16

申请号：US14562046

申请日：2014-12-05

Applicant: Abbott Laboratories

Inventor： Martin Krockenberger ,  Richard Bordenkircher ,  Diana Garrett ,  John Glazier ,  James Bearden ,  Bodo Roemer ,  Giacomo Vacca

IPC: G01N33/00 ,  G01N31/00 ,  G01N33/49 ,  G01N15/14 ,  G01N15/00 ,  G01N15/10

CPC classification number: G01N33/4915 ,  G01N15/1429 ,  G01N15/1459 ,  G01N33/492 ,  G01N2015/0069 ,  G01N2015/008 ,  G01N2015/1006 ,  G01N2015/1486 ,  G01N2015/1488 ,  Y10T436/101666 ,  Y10T436/2575

Abstract: A hematology analyzer is provided. In certain embodiments, the hematology analyzer comprises: a) a flow cell; b) a light source for directing light to the flow cell; c) a plurality of detectors for detecting a plurality of optical characteristics of a blood cell passing through the flow cell; and d) a data analysis workstation programmed to: i. enumerate test blood cells passing through the flow cell; and ii. flag a blood sample as containing lysis-resistant red blood cells or fragile white blood cells.

Abstract translation: 提供血液分析仪。 在某些实施方案中，血液分析仪包括：a）流动池; b）用于将光引导到流动池的光源; c）多个检测器，用于检测通过流通池的血细胞的多个光学特性; 和d）数据分析工作站被编程为：i。 列举通过流动池的测试血液细胞; 和ii。 标记含有裂解抗性红细胞或脆弱白细胞的血液样品。

公开(公告)号：US20160018312A1

公开(公告)日：2016-01-21

申请号：US14808720

申请日：2015-07-24

Applicant: Abbott Laboratories

Inventor： Jiong Wu ,  Marilou Coleman ,  Emily H. Lin ,  Michael R. Buhl ,  Giacomo Vacca

IPC: G01N15/14 ,  G01N21/64 ,  G01N33/49

CPC classification number: G01N15/1434 ,  G01N15/1459 ,  G01N15/147 ,  G01N21/6428 ,  G01N33/49 ,  G01N33/80 ,  G01N2015/0069 ,  G01N2015/008 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2015/1477 ,  G01N2015/1488 ,  G01N2021/6439

Abstract: Systems and methods for analyzing blood samples, and more specifically for performing a nucleated red blood cell (nRBC) analysis. The systems and methods screen a blood sample by means of fluorescence staining and a fluorescence triggering strategy, to identify nuclei-containing particles within the blood sample. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder reagent(s), suitable for assays of samples containing fragile white blood cells (WBCs). In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; (b) using a fluorescence trigger to screen the blood sample for nuclei-containing particles; and (c) using measurements of light scatter and fluorescence emission to distinguish nRBCs from WBCs.

Abstract translation: 用于分析血液样品的系统和方法，更具体地用于进行有核红细胞（nRBC）分析。 系统和方法通过荧光染色和荧光触发策略筛选血液样品，以鉴定血液样品中含有核的颗粒。 因此，基本上消除了来自未分解的红细胞（RBC）和裂解的RBC的片段的干扰。 这些系统和方法还能够开发适合于含有易碎白细胞（WBC）的样品的测定的较温和的试剂。 在一个实施方案中，系统和方法包括：（a）用专用细胞膜可渗透荧光染料染色血液样品; （b）使用荧光触发器来筛选含有核的颗粒的血液样品; 和（c）使用光散射和荧光发射的测量来区分来自WBC的nRBC。