公开(公告)号：US20150038361A1

公开(公告)日：2015-02-05

申请号：US14377962

申请日：2013-02-14

Applicant: Cornell University

Inventor： David Erickson ,  Matthew Mancuso ,  Ethel Cesarman ,  Li Jiang

IPC: C12Q1/70 ,  G01N33/569 ,  G01N33/553 ,  C12Q1/68 ,  G01N33/543

CPC classification number: C12Q1/705 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/689 ,  C12Q1/703 ,  C12Q2565/628 ,  C12Q2600/112 ,  C12Q2600/158 ,  C12Q2600/16 ,  G01N33/5088 ,  G01N33/54313 ,  G01N33/54346 ,  G01N33/553 ,  G01N33/569 ,  G01N33/56911 ,  G01N33/56994 ,  G01N33/587 ,  C12Q2537/143

Abstract: Methods and systems for colorimetric detection of a target. Nucleic acid is obtained from a sample potentially containing two pathogens of interest, and is contacted with a plurality of nanoparticles. A first portion of the plurality of nanoparticles are functionalized with oligonucleotides complementary to a first region of the first target and oligonucleotides complementary to a second region of the first target, and a second portion of the plurality of nanoparticles are functionalized with oligonucleotides complementary to a first region of the second target and oligonucleotides complementary to a second region of the second target. The presence of the target nucleic acid causes a detectable colorimetric change, thereby diagnosing the presence of the pathogen.

Abstract translation: 目标的比色检测方法和系统。 从潜在含有两种感兴趣病原体的样品获得核酸，并与多个纳米颗粒接触。 多个纳米颗粒的第一部分用与第一靶标的第一区域互补的寡核苷酸和与第一靶标的第二区域互补的寡核苷酸功能化，并且多个纳米颗粒的第二部分用与第一靶标互补的寡核苷酸功能化 区域和与第二靶标的第二区域互补的寡核苷酸。 靶核酸的存在导致可检测的比色变化，从而诊断病原体的存在。

公开(公告)号：US09821314B2

公开(公告)日：2017-11-21

申请号：US14429407

申请日：2013-09-18

Applicant: CORNELL UNIVERSITY

Inventor： David Erickson ,  Li Jiang ,  Matthew Mancuso

IPC: C12Q1/68 ,  C12P19/34 ,  B01L7/00 ,  G01N35/00 ,  B01L3/00

CPC classification number: B01L7/52 ,  B01L3/5027 ,  B01L3/502715 ,  B01L7/5255 ,  B01L2300/0803 ,  B01L2300/0816 ,  B01L2300/12 ,  B01L2300/168 ,  B01L2300/1861 ,  C12Q1/686 ,  G01N2035/00158 ,  C12Q2565/629

Abstract: Disclosed are methods and apparatus for solar-thermal microfluidic polymerase chain reaction. A device comprises a microfluidic chip including at least one PCR region, an energy absorption layer disposed adjacent to the microfluidic chip, a solar energy concentrator adapted to produce a plurality of temperature profiles on the microfluidic chip adapted to facilitate PCR, and a photomask disposed between the solar energy concentrator and the microfluidic chip.

公开(公告)号：US20150238967A1

公开(公告)日：2015-08-27

申请号：US14429407

申请日：2013-09-18

Applicant: CORNELL UNIVERSITY

Inventor： David Erickson ,  Li Jiang ,  Matthew Mancuso

IPC: B01L7/00 ,  C12Q1/68 ,  B01L3/00

CPC classification number: B01L7/52 ,  B01L3/5027 ,  B01L3/502715 ,  B01L7/5255 ,  B01L2300/0803 ,  B01L2300/0816 ,  B01L2300/12 ,  B01L2300/168 ,  B01L2300/1861 ,  C12Q1/686 ,  G01N2035/00158 ,  C12Q2565/629

Abstract: Disclosed are methods and apparatus for solar-thermal microfluidic polymerase chain reaction. A device comprises a microfluidic chip including at least one PCR region, an energy absorption layer disposed adjacent to the microfluidic chip, a solar energy concentrator adapted to produce a plurality of temperature profiles on the microfluidic chip adapted to facilitate PCR, and a photomask disposed between the solar energy concentrator and the microfluidic chip.

Abstract translation: 公开了用于太阳热微流体聚合酶链反应的方法和装置。 一种装置包括微流体芯片，其包括至少一个PCR区域，与该微流体芯片相邻设置的能量吸收层，适于在适于促进PCR的微流体芯片上产生多个温度分布的太阳能集中器，以及设置在 太阳能集中器和微流控芯片。