公开(公告)号：US20250153140A1

公开(公告)日：2025-05-15

申请号：US18839374

申请日：2023-02-16

Applicant: Cytiva BioProcess R&D AB

Inventor： Jean-Luc Maloisel ,  Frederic Leroux ,  Lisa Marx

IPC: B01J20/289 ,  B01D15/36 ,  B01D15/38 ,  B01J20/24 ,  B01J20/26 ,  B01J20/28 ,  B01J20/32

Abstract: A chromatography medium is provided, comprising a matrix of cellulose-based nanofibers, the nanofibers optionally being crosslinked to one another. A ligand coupled to the matrix without any intermediate extender group. Also provided is a method of preparing a functionalised chromatography medium. The method comprises: (i) providing a substrate comprising cellulose acetate; (ii) forming a fibrous matrix/membrane spun of nanofibers from the substrate; (iii) saponification of the nanofibers to form regenerated cellulose nanofibers; (iv) derivatisation of the regenerated cellulose nanofibers with a cross-linker, and (v) coupling of a ligand to the derivatised cellulose nanofibers, wherein the preparation of the functionalised chromatography medium does not comprise any surface extender. The chromatography medium is useful for separation of large analytes, such as viruses.

公开(公告)号：US20240261705A1

公开(公告)日：2024-08-08

申请号：US18566551

申请日：2022-06-27

Applicant: Cytiva BioProcess R&D AB

Inventor： Jean-Luc Maloisel ,  Asa Hagner McWhirter ,  Ola Lind ,  Frederic Leroux

IPC: B01D15/36 ,  B01J41/20 ,  C12N7/02

CPC classification number: B01D15/363 ,  B01J41/20 ,  C12N7/02

Abstract: An anion exchange chromatography medium (1) for use in purification of enveloped virus particles or exosomes from a feed, the anion exchange chromatography medium comprising a support material being functionalized with a ligand comprising a diamine functionality generating at least one weak anion exchange group to an ionic capacity of 10-500 μmol/mL.