公开(公告)号：US20250058298A1

公开(公告)日：2025-02-20

申请号：US18712580

申请日：2022-12-06

Applicant: Cytiva BioProcess R&D AB

Inventor： Jean-Luc Maloisel ,  Åsa Hagner McWhirter ,  Ola Lind ,  Ida Helena Vestin ,  Erik Tingborn ,  Björn Norén

IPC: B01J20/28 ,  B01J20/24 ,  B01J20/282 ,  B01J20/32

Abstract: The present disclosure is directed to a separation matrix comprising a plurality of chromatography particles, each chromatography particle comprising a core and a layer surrounding the core, wherein the core has a first average pore diameter and the layer surrounding the core has a second average pore diameter, wherein the second average pore diameter is at least 1.5 times higher than the first average pore diameter, wherein the first average pore diameter excludes diffusion of a target molecule through the pores of the core and wherein the second average pore diameter at least partly permits diffusion of the target molecule through the pores of the layer surrounding the core. Further disclosed are a method for preparing such a separation matrix, uses of such a separation matrix and methods for separating target molecules by use of such a separation matrix, in particular a method for separating adeno associated virus capsids fully packaged with genetic material from adeno associated virus capsids not fully packaged with genetic material, and compositions obtained by said method.

公开(公告)号：US20240261705A1

公开(公告)日：2024-08-08

申请号：US18566551

申请日：2022-06-27

Applicant: Cytiva BioProcess R&D AB

Inventor： Jean-Luc Maloisel ,  Asa Hagner McWhirter ,  Ola Lind ,  Frederic Leroux

IPC: B01D15/36 ,  B01J41/20 ,  C12N7/02

CPC classification number: B01D15/363 ,  B01J41/20 ,  C12N7/02

Abstract: An anion exchange chromatography medium (1) for use in purification of enveloped virus particles or exosomes from a feed, the anion exchange chromatography medium comprising a support material being functionalized with a ligand comprising a diamine functionality generating at least one weak anion exchange group to an ionic capacity of 10-500 μmol/mL.

公开(公告)号：US20240076650A1

公开(公告)日：2024-03-07

申请号：US18260560

申请日：2022-01-26

Applicant: CYTIVA BIOPROCESS R&D AB

Inventor： Peter Lundback ,  Ronnie Palmgren ,  Ola Lind ,  Nils Norrman ,  Helen Cheek ,  Susana Brito Dos Santos ,  Per Denker

IPC: C12N15/10 ,  B01D15/38 ,  B01J20/26 ,  B01J20/28 ,  B01J20/289

CPC classification number: C12N15/101 ,  B01D15/3819 ,  B01J20/265 ,  B01J20/28035 ,  B01J20/28038 ,  B01J20/289 ,  B01J2220/52

Abstract: The invention relates to processes for purification of poly-tagged products, such as mRNA, from synthetic or biological compositions. The process involves contacting the composition with a oligo d (T)-functionalized chromatography medium comprising a convection-based chromatography material.

公开(公告)号：US11053283B2

公开(公告)日：2021-07-06

申请号：US16386628

申请日：2019-04-17

Applicant: Cytiva BioProcess R&D AB

Inventor： Jean-Luc Maloisel ,  Ola Lind ,  Bjorn Noren ,  Ronnie Palmgren

IPC: C07K1/22 ,  C07K16/00 ,  C07K16/06 ,  C07K1/14

Abstract: The present invention relates to a method for removal of large contaminants, such as virus, in a chromatographic process for purification of a target molecule, preferably monoclonal antibodies, mAbs, by using a specifically designed chromatographic bead having a thin outer layer and a core functionalized with a ligand adsorbing the mAbs or parts thereof.