公开(公告)号：US08565480B2

公开(公告)日：2013-10-22

申请号：US12979996

申请日：2010-12-28

Applicant: Ole Eichhorn ,  Greg Crandall ,  Steven Hashagen ,  Dirk Soenksen ,  Mark Wrenn

Inventor： Ole Eichhorn ,  Greg Crandall ,  Steven Hashagen ,  Dirk Soenksen ,  Mark Wrenn

IPC: G06K9/00

CPC classification number: G02B27/2278 ,  G02B21/367 ,  G06K9/00201 ,  H04N13/395

Abstract: Systems and methods for creating and viewing three dimensional digital slides are provided. One or more microscope slides are positioned in an image acquisition device that scans the specimens on the slides and makes two dimensional images at a medium or high resolution. These two dimensional digital slide images are provided to an image viewing workstation where they are viewed by an operator who pans and zooms the two dimensional image and selects an area of interest for scanning at multiple depth levels (Z-planes). The image acquisition device receives a set of parameters for the multiple depth level scan, including a location and a depth. The image acquisition device then scans the specimen at the location in a series of Z-plane images, where each Z-plane image corresponds to a depth level portion of the specimen within the depth parameter.

公开(公告)号：US08165363B2

公开(公告)日：2012-04-24

申请号：US12114627

申请日：2008-05-02

Applicant: Dirk Soenksen ,  Kathy Zirker-Smith

Inventor： Dirk Soenksen ,  Kathy Zirker-Smith

IPC: G06K9/00

CPC classification number: G06K9/00127 ,  G02B21/365 ,  G06F19/00 ,  G06F19/30 ,  G06K9/00134 ,  G06K9/03 ,  G06K9/033 ,  G06Q50/22 ,  G06T5/001 ,  G06T7/0002 ,  G06T7/0012 ,  G06T2207/10024 ,  G06T2207/10056 ,  G06T2207/30024

Abstract: Systems and methods for improving quality assurance in pathology using automated quality assessment and digital image enhancements on digital slides prior to analysis by the pathologist are provided. A digital pathology system (slide scanning instrument and software) creates, assesses and improves the quality of a digital slide. The improved digital slide image has a higher image quality that results in increased efficiency and accuracy in the analysis and diagnosis of such digital slides when they are reviewed on a monitor by a pathologist. These improved digital slides yield a more objective diagnosis than reading the corresponding glass slide under a microscope.

Abstract translation: 提供了在病理学家分析之前，通过使用自动化质量评估和数字图像增强技术，改善病理学质量保证的系统和方法。 数字病理系统（幻灯片扫描仪器和软件）创建，评估和提高数字幻灯片的质量。 改进的数字幻灯片图像具有更高的图像质量，当病理学家在监视器上进行审查时，可以提高这种数字幻灯片的分析和诊断的效率和准确性。 这些改进的数字幻灯片比在显微镜下阅读相应的载玻片更容易得到客观的诊断。

公开(公告)号：US07689024B2

公开(公告)日：2010-03-30

申请号：US12330370

申请日：2008-12-08

Applicant: Ole Eichhorn ,  Greg Crandall ,  Dirk Soenksen ,  Steven Hashagen ,  Mark Wrenn

Inventor： Ole Eichhorn ,  Greg Crandall ,  Dirk Soenksen ,  Steven Hashagen ,  Mark Wrenn

IPC: G06K9/00

CPC classification number: G02B27/2278 ,  G02B21/367 ,  G06K9/00201 ,  H04N13/395

Abstract: Systems and methods for creating and viewing three dimensional virtual slides are provided. One or more microscope slides are positioned in an image acquisition device that scans the specimens on the slides and makes two dimensional images at a medium or high resolution. This two dimensional images are provided to an image viewing workstation where they are viewed by an operator who pans and zooms the two dimensional image and selects an area of interest for scanning at multiple depth levels (Z-planes). The image acquisition device receives a set of parameters for the multiple depth level scan, including a location and a depth. The image acquisition device then scans the specimen at the location in a series of Z-plane images, where each Z-plane image corresponds to a depth level portion of the specimen within the depth parameter.

Abstract translation: 提供了用于创建和查看三维虚拟幻灯片的系统和方法。 将一个或多个显微镜载片放置在图像采集装置中，扫描载玻片上的标本，并以中等或高分辨率制作二维图像。 该二维图像被提供给图像查看工作站，在那里它们被操作者观看，该操作者平移和缩放二维图像并选择感兴趣的区域以在多个深度级别（Z平面）进行扫描。 图像采集装置接收包括位置和深度的多个深度级扫描的一组参数。 然后，图像获取装置在一系列Z平面图像中的位置处扫描样本，其中每个Z平面图像对应于深度参数内的样本的深度级部分。

公开(公告)号：US07463761B2

公开(公告)日：2008-12-09

申请号：US11140265

申请日：2005-05-27

Applicant: Ole Eichhorn ,  Greg Crandall ,  Steven Hashagen ,  Dirk Soenksen ,  Mark Wrenn

Inventor： Ole Eichhorn ,  Greg Crandall ,  Steven Hashagen ,  Dirk Soenksen ,  Mark Wrenn

IPC: G06K9/00

CPC classification number: G02B27/2278 ,  G02B21/367 ,  G06K9/00201 ,  H04N13/395

Abstract: Systems and methods for creating and viewing three dimensional virtual slides are provided. One or more microscope slides are positioned in an image acquisition device that scans the specimens on the slides and makes two dimensional images at a medium or high resolution. This two dimensional images are provided to an image viewing workstation where they are viewed by an operator who pans and zooms the two dimensional image and selects an area of interest for scanning at multiple depth levels (Z-planes). The image acquisition device receives a set of parameters for the multiple depth level scan, including a location and a depth. The image acquisition device then scans the specimen at the location in a series of Z-plane images, where each Z-plane image corresponds to a depth level portion of the specimen within the depth parameter.

Abstract translation: 提供了用于创建和查看三维虚拟幻灯片的系统和方法。 将一个或多个显微镜载片放置在图像采集装置中，扫描载玻片上的标本，并以中等或高分辨率制作二维图像。 该二维图像被提供给图像查看工作站，在那里它们被操作者观看，该操作者平移和缩放二维图像并选择感兴趣的区域以在多个深度级别（Z平面）进行扫描。 图像采集装置接收包括位置和深度的多个深度级扫描的一组参数。 然后，图像获取装置在一系列Z平面图像中的位置处扫描样本，其中每个Z平面图像对应于深度参数内的样本的深度级部分。

公开(公告)号：US20070036462A1

公开(公告)日：2007-02-15

申请号：US11379648

申请日：2006-04-21

Applicant: Greg Crandall ,  Ole Eichhorn ,  Allen Olson ,  Dirk Soenksen

Inventor： Greg Crandall ,  Ole Eichhorn ,  Allen Olson ,  Dirk Soenksen

IPC: G06K9/36 ,  G06K9/32 ,  G06K9/00

CPC classification number: G02B21/367 ,  G02B21/002 ,  G02B21/0036 ,  G02B21/008 ,  G02B21/14 ,  G02B21/245 ,  G02B21/26 ,  G02B21/32 ,  G02B21/34 ,  G02B21/365 ,  G06F19/00 ,  H04N1/053 ,  H04N1/1903 ,  H04N1/193 ,  H04N1/19573 ,  H04N5/2256 ,  H04N5/23206 ,  H04N7/18 ,  H04N7/183 ,  H04N2201/04727 ,  H04N2201/04755

Abstract: A data management system and method for processing, storing, and viewing the extremely large imagery data that is rapidly produced by a linear-array-based microscope slide scanner is provided. The system receives, processes, and stores imagery data produced by the linear-array-based microscope slide scanner at approximately 3 GB per minute. The data are received as a series of overlapping image stripes and combined into a seamless and contiguous baseline image. The baseline image is logically mapped into a plurality of regions that are individually addressed to facilitate viewing and manipulation of the baseline image. The data management system enables imagery data compression while scanning and capturing new image stripes. This advantageously eliminates the overhead associated with storing uncompressed image stripes. The image compression also creates intermediate level images, thereby organizing the baseline image into a variable level pyramid structure referred to as a virtual slide. The data management system efficiently converts image stripes into a high quality virtual slide that allows rapid panning and zooming by image viewing software in accordance with the individually addressed regions. The virtual slide also allows efficient processing by an algorithm framework. The data management system is costs effective and scaleable, employs standard image file formats and supports the use of virtual slides in desirable applications such as telemedicine, telepathology, microscopy education, and the analysis of high value specimens such as tissue arrays.

Abstract translation: 提供了一种用于处理，存储和查看由基于线性阵列的显微镜幻灯片扫描仪快速产生的极大图像数据的数据管理系统和方法。 系统以每分钟3 GB的速度接收，处理和存储由基于线性阵列的显微镜幻灯片扫描仪生成的图像数据。 数据被接收为一系列重叠的图像条纹并组合成无缝和连续的基线图像。 基线图像被逻辑地映射到单独寻址的多个区域中，以便于观察和操纵基线图像。 数据管理系统在扫描和捕获新的图像条纹时可以进行图像数据压缩。 这有利地消除了与存储未压缩图像条纹相关联的开销。 图像压缩还创建中间级图像，从而将基线图像组织成称为虚拟幻灯片的可变级金字塔结构。 数据管理系统有效地将图像条纹转换成高质量的虚拟幻灯片，其允许通过图像查看软件根据单独寻址的区域进行快速平移和缩放。 虚拟幻灯片还允许通过算法框架进行有效的处理。 数据管理系统具有成本效益和可扩展性，采用标准图像文件格式，并支持虚拟幻灯片在远程医疗，远程病理学，显微镜教育等理想应用中的应用，以及组织阵列等高价值标本的分析。

公开(公告)号：US20060245634A1

公开(公告)日：2006-11-02

申请号：US10564454

申请日：2004-06-03

Applicant: Dirk Soenksen ,  Ralf Friedrich ,  Andreas Draeger ,  Detlef Schupp ,  Thin Van Luu ,  Wolfgang Langer

Inventor： Dirk Soenksen ,  Ralf Friedrich ,  Andreas Draeger ,  Detlef Schupp ,  Thin Van Luu ,  Wolfgang Langer

IPC: G06K9/00 ,  G06K9/62

CPC classification number: G01N21/9501 ,  G01N2021/8854 ,  G06T7/0004 ,  G06T2207/30148

Abstract: The invention relates to a method of learning a knowledge-based database used in automatic defect classification. According to this method, the user is spared a series of entries as the system carries out an automatic learn mode, which requires a reduced number of user entries.

公开(公告)号：US20060007345A1

公开(公告)日：2006-01-12

申请号：US11140589

申请日：2005-05-26

Applicant: Allen Olson ,  Kiran Saligrama ,  Dirk Soenksen

Inventor： Allen Olson ,  Kiran Saligrama ,  Dirk Soenksen

IPC: G03B13/00 ,  H04N5/232

CPC classification number: G02B21/245 ,  G02B21/002 ,  G02B21/244 ,  G02B21/365 ,  G02B21/367 ,  G06K9/40 ,  H04N5/23212

Abstract: Systems and methods for assessing virtual microscope slide image quality are provided. In order to determine whether a virtual slide image has any out of focus areas and is therefore a candidate for manual inspection, the various focus points used to scan the virtual slide image are used to calculate a best fit surface for the virtual slide image. The distance of each focus point from the best fit surface is then calculated and the largest distance is compared to a predetermined value. If the largest distance from a focus point to the best fit surface is larger than the predetermined value, then the virtual slide image is designated as needing a manual inspection and possible re-scan.

Abstract translation: 提供了评估虚拟显微镜幻灯片图像质量的系统和方法。 为了确定虚拟幻灯片图像是否具有任何偏离焦点区域，因此是用于手动检查的候选者，用于扫描虚拟幻灯片图像的各种对焦点用于计算虚拟幻灯片图像的最佳拟合曲面。 然后计算每个对焦点与最佳拟合表面的距离，并将最大距离与预定值进行比较。 如果从焦点到最佳配合曲面的最大距离大于预定值，则虚拟幻灯片图像被指定为需要手动检查和可能的重新扫描。

公开(公告)号：US6165734A

公开(公告)日：2000-12-26

申请号：US196690

申请日：1998-11-20

Applicant: Yuval Garini ,  George McNamara ,  Dirk Soenksen ,  Dario Cabib ,  Robert A Buckwald

Inventor： Yuval Garini ,  George McNamara ,  Dirk Soenksen ,  Dario Cabib ,  Robert A Buckwald

IPC: C12Q1/68 ,  G01J3/02 ,  G01J3/12 ,  G01J3/26 ,  G01J3/28 ,  G01J3/44 ,  G01J3/453 ,  G01N1/30 ,  G01N21/64 ,  G01N21/78 ,  G01N33/50 ,  G01N33/53 ,  G01N33/533 ,  G01N33/535 ,  G01N33/569 ,  G01N33/58 ,  G06K9/00 ,  G06K9/76 ,  G01N33/567 ,  C12Q1/42 ,  C12Q1/54

CPC classification number: C12Q1/6841 ,  C12Q1/6883 ,  G01J3/02 ,  G01J3/12 ,  G01J3/1256 ,  G01J3/26 ,  G01J3/2823 ,  G01J3/4406 ,  G01J3/453 ,  G01J2003/2866 ,  G01N1/30 ,  G01N21/6428 ,  G01N21/6456 ,  G01N33/5005 ,  G01N33/53 ,  G01N33/56966 ,  G01N33/582 ,  G01N2021/6423 ,  G01N2021/6441 ,  G06K9/00127 ,  G06K9/76 ,  Y10S435/968

Abstract: A method of in situ analysis of a biological sample comprising the steps of (a) staining the biological sample with N stains of which a first stain is selected from the group consisting of a first immunohistochemical stain, a first histological stain and a first DNA ploidy stain, and a second stain is selected from the group consisting of a second immunohistochemical stain, a second histological stain and a second DNA ploidy stain, with provisions that N is an integer greater than three and further that (i) if the first stain is the first immunohistochemical stain then the second stain is either the second histological stain or the second DNA ploidy stain; (ii) if the first stain is the first histological stain then the second stain is either the second immunohistochemical stain or the second DNA ploidy stain; whereas (iii) if the first stain is the first DNA ploidy stain then the second stain is either the second immunohistochemical stain or the second histological stain; and (b) using a spectral data collection device for collecting spectral data from the biological sample, the spectral data collection device and the N stains are selected such that a spectral component associated with each of the N stains is collectable.

Abstract translation: 一种生物样品的原位分析方法，包括以下步骤：（a）染色具有N染色的生物样品，其中第一染色选自第一免疫组织化学染色，第一组织染色和第一DNA倍体 染色，第二染色选自第二免疫组织化学染色，第二组织学染色和第二DNA倍性染色，其规定N为大于3的整数，并且（i）如果第一染色是 第一次免疫组织化学染色，第二次染色是第二次组织染色或第二次DNA倍体染色; （ii）如果第一染色是第一组织染色，则第二染色是第二免疫组织化学染色或第二DNA倍体染色; 而（iii）如果第一个染色是第一个DNA倍性染色，则第二次染色是第二次免疫组化染色或第二次组织染色; 和（b）使用用于收集来自生物样品的光谱数据的光谱数据采集装置，选择光谱数据采集装置和N染色，使得与每个N染色体相关联的光谱分量是可收集的。

公开(公告)号：US07623698B2

公开(公告)日：2009-11-24

申请号：US10564454

申请日：2004-06-03

Applicant: Dirk Soenksen ,  Ralf Friedrich ,  Andreas Draeger ,  Detlef Schupp ,  Thin Van Luu ,  Wolfgang Langer

Inventor： Dirk Soenksen ,  Ralf Friedrich ,  Andreas Draeger ,  Detlef Schupp ,  Thin Van Luu ,  Wolfgang Langer

IPC: G06K9/00

CPC classification number: G01N21/9501 ,  G01N2021/8854 ,  G06T7/0004 ,  G06T2207/30148

Abstract: The invention relates to a method of learning a knowledge-based database used in automatic defect classification. According to this method, the user is spared a series of entries as the system carries out an automatic learn mode, which requires a reduced number of user entries.

Abstract translation: 本发明涉及一种学习在自动缺陷分类中使用的基于知识的数据库的方法。 根据该方法，当系统执行自动学习模式时，用户不需要一系列条目，这需要减少用户条目数量。

公开(公告)号：US20090116733A1

公开(公告)日：2009-05-07

申请号：US12330370

申请日：2008-12-08

Applicant: Ole Eichhorn ,  Greg Crandall ,  Steven Hashagen ,  Dirk Soenksen ,  Mark Wrenn

Inventor： Ole Eichhorn ,  Greg Crandall ,  Steven Hashagen ,  Dirk Soenksen ,  Mark Wrenn

IPC: G06K9/00

CPC classification number: G02B27/2278 ,  G02B21/367 ,  G06K9/00201 ,  H04N13/395

Abstract: Systems and methods for creating and viewing three dimensional virtual slides are provided. One or more microscope slides are positioned in an image acquisition device that scans the specimens on the slides and makes two dimensional images at a medium or high resolution. This two dimensional images are provided to an image viewing workstation where they are viewed by an operator who pans and zooms the two dimensional image and selects an area of interest for scanning at multiple depth levels (Z-planes). The image acquisition device receives a set of parameters for the multiple depth level scan, including a location and a depth. The image acquisition device then scans the specimen at the location in a series of Z-plane images, where each Z-plane image corresponds to a depth level portion of the specimen within the depth parameter.

Abstract translation: 提供了用于创建和查看三维虚拟幻灯片的系统和方法。 将一个或多个显微镜载片放置在图像采集装置中，扫描载玻片上的标本，并以中等或高分辨率制作二维图像。 该二维图像被提供给图像查看工作站，在那里它们被操作者观看，该操作者平移和缩放二维图像并选择感兴趣的区域以在多个深度级别（Z平面）进行扫描。 图像采集装置接收包括位置和深度的多个深度级扫描的一组参数。 然后，图像获取装置在一系列Z平面图像中的位置处扫描样本，其中每个Z平面图像对应于深度参数内的样本的深度级部分。