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    Method of and composite for in situ fluorescent hybridization
    3.
    发明授权
    Method of and composite for in situ fluorescent hybridization 失效
    原位荧光杂交法及复合方法

    公开(公告)号：US6043039A

    公开(公告)日：2000-03-28

    申请号：US122661

    申请日：1998-07-27

    Applicant: Irit Bar-Am ,  Cathy Janish ,  Yuval Garini ,  Dario Cabib ,  Robert A. Buckwald

    Inventor： Irit Bar-Am ,  Cathy Janish ,  Yuval Garini ,  Dario Cabib ,  Robert A. Buckwald

    IPC: G01N33/53 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/533 ,  G01N33/566

    CPC classification number: C12Q1/6841

    Abstract: A fluorescent in situ hybridization method including the steps of (a) obtaining a chromosome spread of a species; (b) preparing a hybridization composite containing a plurality of chromosomal paints each of the plurality of chromosomal paints being labeled with a different fluorophore-or-combination-of-fluorophores, such that an averaged specific activity of highly repetitive sequences in the hybridization composite substantially equals an averaged specific activity of unique sequences in the hybridization composite; (c) denaturing the hybridization composite and subjecting the hybridization composite to conditions for allowing at least a part of the highly repetitive sequences in the hybridization composite to reanneal while at least a part of the unique sequences in the hybridization composite remaining single stranded; (d) contacting under hybridization conditions the hybridization composite with the chromosome spread; (e) washing away excess of the hybridization composite; and (d) analyzing and presenting images of the now hybridized chromosome spread.

    Abstract translation: 一种荧光原位杂交方法，包括以下步骤：（a）获得物种的染色体扩散; （b）制备含有多个染色体涂料的杂化复合物，所述多个染色体涂料中的每一个用不同的荧光团或荧光团组合进行标记，使得所述杂交复合物中高度重复序列的平均比活性基本上 等于杂交复合物中独特序列的平均比活性; （c）使杂交复合物变性并对杂交复合物进行条件以允许杂交复合物中的至少一部分高度重复序列进行再冷凝，同时杂交复合物中至少一部分独特序列保持单链; （d）在杂交条件下使杂交复合物与染色体扩散接触; （e）洗去杂交组合物的过量; 和（d）分析和呈现现在杂交的染色体扩增的图像。

    Spectral bio-imaging methods for biological research, medical diagnostics and therapy
    4.
    发明授权
    Spectral bio-imaging methods for biological research, medical diagnostics and therapy 失效
    用于生物研究，医学诊断和治疗的光谱生物成像方法

    公开(公告)号：US5784162A

    公开(公告)日：1998-07-21

    申请号：US571047

    申请日：1995-12-12

    Applicant: Dario Cabib ,  Robert A. Buckwald ,  Zvi Malik ,  Yuval Garini ,  Nir Katzir ,  Dirk G. Soeknsen

    Inventor： Dario Cabib ,  Robert A. Buckwald ,  Zvi Malik ,  Yuval Garini ,  Nir Katzir ,  Dirk G. Soeknsen

    IPC: G01B11/00 ,  A61B3/00 ,  A61B3/12 ,  A61B5/00 ,  A61B10/00 ,  C12Q1/68 ,  G01J3/02 ,  G01J3/12 ,  G01J3/26 ,  G01J3/28 ,  G01J3/44 ,  G01J3/443 ,  G01J3/45 ,  G01J3/453 ,  G01N21/27 ,  G01N21/64 ,  G01N33/48 ,  G01N33/483 ,  G01N33/50 ,  G01N33/569 ,  G01N33/58 ,  G02B21/36 ,  G06K9/00 ,  G06K9/76 ,  G01B9/02

    CPC classification number: G01N21/6458 ,  C12Q1/6841 ,  C12Q1/6883 ,  G01J3/12 ,  G01J3/1256 ,  G01J3/26 ,  G01J3/2823 ,  G01J3/4406 ,  G01J3/453 ,  G01N21/6428 ,  G01N21/6486 ,  G01N33/5005 ,  G01N33/56966 ,  G01N33/582 ,  G06K9/00127 ,  G06K9/76 ,  G01J2003/2866 ,  G01J3/02 ,  G01N2021/6417 ,  G01N2021/6423

    Abstract: According to the present invention there are provided spectral imaging methods for biological research, medical diagnostics and therapy comprising the steps of (a) preparing a sample to be spectrally imaged; (b) viewing the sample through an optical device, the optical device being optically connected to an imaging spectrometer, the optical device and the imaging spectrometer obtaining a spectrum of each pixel of the sample by: (i) collecting incident light simultaneously from all pixels of the sample using collimating optics; (ii) passing the incident collimated light through an interferometer system having a number of elements, to form an exiting light beam; (iii) passing the exiting light beam through a focusing optical system which focuses the exiting light beam on a detector having a two-dimensional array of detector elements, so that at each instant each of the detector elements is the image of one pixel of the sample, so that the real image of the sample is stationary on the plane of the detector array, and so that each of the detector elements produces a signal which is a particular linear combination of light intensity emitted by the pixel at different wavelengths, wherein the linear combination is a function of the instantaneous optical path difference; (iv) rotating one or more of the elements of the interferometer system, so that the optical path difference between the two coherent beams generated by the interferometer system is scanned simultaneously for all the pixels of the sample; and (v) recording signals of each of the detector elements as function of time using a recording device to form a first spectral cube of data; and (c) interpreting the first spectral cube of data using a mathematical algorithm.

    Abstract translation: 根据本发明，提供了用于生物学研究，医学诊断和治疗的光谱成像方法，其包括以下步骤：（a）制备待成像的样品; （b）通过光学装置观察样品，光学装置光学连接到成像光谱仪，光学装置和成像光谱仪通过以下步骤获得样品的每个像素的光谱：（i）从所有像素同时收集入射光 的样品使用准直光学; （ii）使入射的准直光通过具有多个元件的干涉仪系统，以形成出射的光束; （iii）使出射的光束通过聚焦光学系统，该聚焦光学系统将出射的光束聚焦在具有检测器元件的二维阵列的检测器上，使得在每个瞬间，每个检测器元件是 样本，使得样本的真实图像在检测器阵列的平面上是静止的，并且使得每个检测器元件产生信号，其是由不同波长的像素发射的光强度的特定线性组合，其中 线性组合是瞬时光程差的函数; （iv）旋转干涉仪系统的一个或多个元件，使得由干涉仪系统产生的两个相干光束之间的光程差同时扫描样品的所有像素; 以及（v）使用记录装置记录每个检测器元件的信号作为时间的函数，以形成数据的第一光谱立方体; 和（c）使用数学算法解释数据的第一个频谱立方体。

    Optical microscope and method for obtaining an optical image
    5.
    发明申请
    Optical microscope and method for obtaining an optical image 审中-公开
    光学显微镜和获得光学图像的方法

    公开(公告)号：US20060250686A1

    公开(公告)日：2006-11-09

    申请号：US11393523

    申请日：2006-03-29

    Applicant: Yuval Garini ,  Ian Young

    Inventor： Yuval Garini ,  Ian Young

    IPC: G02B21/00

    CPC classification number: G01Q60/22 ,  G02B21/0032 ,  G02B21/06

    Abstract: The invention relates to an optical microscope, comprising, at least a light source, a carrier for an object to be examined, a detector for registering the illuminated object, and a light path that during operation runs substantially from the light source to the object and form the object to the detector, wherein a metallic film having a periodic hole array is placed in the light path between the light source and the object, and wherein the carrier of the object is provided with a drive to allow the same to be adjusted in the plane of the carrier, wherein the holes of the metallic thin film have a diameter that is smaller than approximately 250 nm, in that the drive is designed for adjusting the carrier for the object in an orientation perpendicular to the plane of the carrier, and in that a processing device is provided that is connected with the detector for constructing a three-dimensional image of the object.

    Abstract translation: 本发明涉及一种光学显微镜，包括至少一个光源，用于待检测物体的载体，用于登记照射物体的检测器和在操作期间基本上从光源到物体运行的光路，以及 形成物体到检测器，其中具有周期性孔阵列的金属膜被放置在光源和物体之间的光路中，并且其中物体的载体设置有驱动器，以允许其被调节 载体的平面，其中金属薄膜的孔具有小于约250nm的直径，因为驱动器设计成用于在垂直于载体的平面的方向上调整用于物体的载体，以及 因为提供了与用于构建物体的三维图像的检测器连接的处理装置。

    Method of and composite for fluorescent in situ hybridization
    6.
    发明授权
    Method of and composite for fluorescent in situ hybridization 失效
    原位荧光杂交法及复合方法

    公开(公告)号：US5871932A

    公开(公告)日：1999-02-16

    申请号：US25131

    申请日：1998-02-17

    Applicant: Irit Bar-Am ,  Yuval Garini ,  Dario Cabib

    Inventor： Irit Bar-Am ,  Yuval Garini ,  Dario Cabib

    IPC: C12Q1/68 ,  C07H21/04

    CPC classification number: C12Q1/6841

    Abstract: A fluorescent in situ hybridization method including the steps of (a) obtaining a chromosome spread of a species; (b) preparing a hybridization composite containing a plurality of chromosomal paints each of the plurality of chromosomal paints being labeled with a different fluorophore-or-combination-of-fluorohores, such that an averaged specific activity of highly repetitive sequences in the hybridization composite substantially equals an averaged specific activity of unique sequences in the hybridization composite; (c) denaturing the hybridization composite and subjecting the hybridization composite to conditions for allowing at least a part of the highly repetitive sequences in the hybridization composite to reanneal while at least a part of the unique sequences in the hybridization composite remaining single stranded; (d) contacting under hybridization conditions the hybridization composite with the chromosome spread; (e) washing away excess of the hybridization composite; and (d) analyzing and presenting images of the now hybridized chromosome spread.

    Abstract translation: 一种荧光原位杂交方法，包括以下步骤：（a）获得物种的染色体扩散; （b）制备含有多个染色体涂料的杂化复合物，所述多个染色体涂料中的每一个都用不同的荧光团或组合的荧光体标记，使得杂交复合物中高度重复序列的平均比活性基本上 等于杂交复合物中独特序列的平均比活性; （c）使杂交复合物变性并对杂交复合物进行条件以允许杂交复合物中的至少一部分高度重复序列进行再冷凝，同时杂交复合物中至少一部分独特序列保持单链; （d）在杂交条件下使杂交复合物与染色体扩散接触; （e）洗去杂交组合物的过量; 和（d）分析和呈现现在杂交的染色体扩增的图像。

    Method for classification of pixels into groups according to their spectra using a plurality of wide band filters and hardwire therefore
    7.
    发明授权
    Method for classification of pixels into groups according to their spectra using a plurality of wide band filters and hardwire therefore 失效
    因此，使用多个宽带滤波器和硬件根据其频谱将像素分类为组的方法

    公开(公告)号：US5834203A

    公开(公告)日：1998-11-10

    申请号：US917213

    申请日：1997-08-25

    Applicant: Nir Katzir ,  David Wine ,  Yuval Garini ,  Dario Cabib

    Inventor： Nir Katzir ,  David Wine ,  Yuval Garini ,  Dario Cabib

    IPC: G01N21/64 ,  G01N15/14 ,  G01N21/78 ,  G01N33/533 ,  G01N33/566 ,  G01N33/58 ,  G02B21/06 ,  C12Q1/68 ,  F21V9/08 ,  G02B21/16

    CPC classification number: G01N21/6428 ,  G01N21/6458 ,  G02B21/06 ,  G01N15/1475 ,  G01N2021/6441

    Abstract: A method of classification of pixels into groups of pixels according to their association with a single fluorophore or a combination of fluorophores selected from a plurality of fluorophores, each of the fluorophores having characterizing excitation and emission spectra and specifying excitation and emission peaks, the method comprising the steps of (a) providing a plurality of pairs of wide-band excitation filters and wide-band emission filters; (b) exciting fluorophores of each of the pixels with light filtered through one of the wide-band excitation filters, and recording emitted light intensity as retrieved after passing through its paired emission filter; (c) repeating step (b) for all of the plurality of pairs of filters, such that each of the pixels is representable by a vector of a plurality of dimensions, the number of dimensions being equal to the number of the plurality of pairs of filters; (c) using an algorithm for evaluating the presence of each of the plurality of fluorophores in each of the pixels, thereby classifying each of the pixels into a group of pixels according to its association with a single fluorophore or combination of fluorophores.

    Abstract translation: 根据其与单个荧光团或选自多个荧光团的荧光团的组合的关联或每个荧光团具有表征激发和发射光谱以及指定激发和发射峰值的方式，将像素分类为像素组的方法，所述方法包括： （a）提供多对宽带激励滤波器和宽带发射滤波器的步骤; （b）每个像素的激发荧光团，其中通过一个宽带激发滤光器之一滤光，并且记录经过其成对发射滤光片之后检索的发射光强度; （c）对于所有多对滤波器重复步骤（b），使得每个像素可以由多个维度的向量表示，维数等于多对滤波器的数量 过滤器 （c）使用用于评估每个像素中的每个多个荧光团的存在的算法，从而根据其与单个荧光团或荧光团的组合的关联将每个像素分类为一组像素。

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