公开(公告)号：US11268127B2

公开(公告)日：2022-03-08

申请号：US15116750

申请日：2015-02-04

Applicant: Gabriel P. Lopez ,  Ashutosh Chilkoti ,  Ali Ghoorchian ,  Felipe Garcia Quiroz ,  Duke University

Inventor： Gabriel P. Lopez ,  Ashutosh Chilkoti ,  Ali Ghoorchian ,  Felipe Garcia Quiroz

IPC: C12Q1/37 ,  G01N33/573

Abstract: The presently disclosed subject matter relates to modular peptide-substrate protease assays, in particular low cost and reliable methodology for measurement of protease and other enzyme activity that may be detected by optical turbidimetry or visual observation. The presently disclosed subject matter also relates to methods of using the disclosed assays within methods of detecting and monitoring diseases, methods of drug discovery, as well as in detection devices and systems.

公开(公告)号：US20160271262A1

公开(公告)日：2016-09-22

申请号：US15074132

申请日：2016-03-18

Applicant: Duke University

Inventor： Gabriel P. Lopez ,  Ashutosh Chilkoti ,  Ali Ghoorchian ,  Joseph R. Simon

IPC: A61K47/48 ,  A61K9/06

CPC classification number: A61K47/64 ,  A61K9/06 ,  A61K47/6907 ,  A61L27/227 ,  A61L27/52 ,  A61L2300/252 ,  A61L2300/626 ,  C07K14/78 ,  C08J3/075 ,  C08J2389/00

Abstract: The presently disclosed subject matter is directed to compositions, methods, and systems (e.g., platforms) comprising the same, the systems comprising, consisting of, or consisting essentially of micro- and macro-hydrogels that are formed from polypeptide micelles. The systems have enhanced mechanical properties that can be useful in a wide variety of applications, can be used for controlled release of drug-loaded micelles, and can be designed to reversibly assemble and disassemble on demand.

Abstract translation: 目前公开的主题涉及包含其的组合物，方法和系统（例如，平台），所述系统包含由多肽胶束形成的微水凝胶和大水凝胶，或由其组成。 这些系统具有增强的机械性能，其可用于各种应用中，可用于控制释放载药胶束，并且可以设计成可按要求可逆地组装和拆卸。

公开(公告)号：US09956300B2

公开(公告)日：2018-05-01

申请号：US15074132

申请日：2016-03-18

Applicant: Duke University

Inventor： Gabriel P. Lopez ,  Ashutosh Chilkoti ,  Ali Ghoorchian ,  Joseph R. Simon

IPC: A61K47/00 ,  C07K14/00 ,  A61K47/48 ,  A61K9/06 ,  C08J3/075 ,  A61L27/22 ,  A61L27/52 ,  C07K14/78 ,  A61K47/64 ,  A61K47/69

CPC classification number: A61K47/64 ,  A61K9/06 ,  A61K47/6907 ,  A61L27/227 ,  A61L27/52 ,  A61L2300/252 ,  A61L2300/626 ,  C07K14/78 ,  C08J3/075 ,  C08J2389/00

Abstract: The presently disclosed subject matter is directed to compositions, methods, and systems (e.g., platforms) comprising the same, the systems comprising, consisting of, or consisting essentially of micro- and macro-hydrogels that are formed from polypeptide micelles. The systems have enhanced mechanical properties that can be useful in a wide variety of applications, can be used for controlled release of drug-loaded micelles, and can be designed to reversibly assemble and disassemble on demand.

公开(公告)号：US20160348147A1

公开(公告)日：2016-12-01

申请号：US15116750

申请日：2015-02-04

Applicant: DUKE UNIVERSITY

Inventor： Gabriel P. Lopez ,  Ashutosh Chilkoti ,  Ali Ghoorchian ,  Felipe Garcia

IPC: C12Q1/37

CPC classification number: C12Q1/37 ,  G01N33/573

Abstract: The presently disclosed subject matter relates to modular peptide-substrate protease assays, in particular low cost and reliable methodology for measurement of protease and other enzyme activity that may be detected by optical turbidimetry or visual observation. The presently disclosed subject matter also relates to methods of using the disclosed assays within methods of detecting and monitoring diseases, methods of drug discovery, as well as in detection devices and systems.

Abstract translation: 目前公开的主题涉及模块化肽 - 底物蛋白酶测定，特别是用于测量蛋白酶和其它酶活性的低成本和可靠的方法，其可以通过光学比浊法或目视观察来检测。 目前公开的主题还涉及在检测和监测疾病的方法，药物发现的方法以及检测装置和系统中使用所公开的测定法的方法。