公开(公告)号：US20220186303A1

公开(公告)日：2022-06-16

申请号：US17383716

申请日：2021-07-23

Applicant: GenapSys, Inc.

Inventor： Hesaam Esfandyarpour ,  Maryam Jouzi ,  Seth Stern ,  Paul Kenney

IPC: C12Q1/6869 ,  C12Q1/6825

Abstract: The present disclosure provides methods and systems for sequencing nucleic acid molecules. Methods may include sequencing double-stranded nucleic acids or single-stranded nucleic acids. Sequencing may include the use of nucleotides coupled to electrostatic moieties. The electrostatic moieties may be detected by a sensor array. The electrostatic moieties may be reversible electrostatic moieties that are cleaved from the nucleic acid molecule after incorporation of the nucleotide. The electrostatic moieties may be irreversible electrostatic moieties. Nucleotides comprising irreversible electrostatic moieties may be incorporated into the nucleic acid molecule, detected by the sensor array, and exchanged for non-detectable nucleotides.

公开(公告)号：US20200232024A1

公开(公告)日：2020-07-23

申请号：US16712601

申请日：2019-12-12

Applicant: GenapSys, Inc.

Inventor： Hesaam Esfandyarpour ,  Kosar Parizi ,  Saurabh Paliwal ,  Seth Stern ,  Paul Kenney ,  Meysam R. Barmi ,  Ali Nabi ,  Hamid Rategh

IPC: C12Q1/6869

Abstract: Provided herein are systems and methods for processing and analyzing nucleic acids and other biomolecules. Methods may include processing nucleic acid molecules in an emulsion of droplets. Methods of analyzing nucleic acid molecules may include coupling nucleic acids to a bead or other support. Methods may include analysis of nucleic acid molecules using a redox mediator. In some cases, analysis of the nucleic acid molecule includes determining a nucleotide sequence of the nucleic acid molecule.

公开(公告)号：US20190256903A1

公开(公告)日：2019-08-22

申请号：US16141215

申请日：2018-09-25

Applicant: GenapSys, Inc.

Inventor： Hesaam Esfandyarpour ,  Maryam Jouzi ,  Seth Stern ,  Paul Kenney

IPC: C12Q1/6869 ,  C12Q1/6825

Abstract: The present disclosure provides methods and systems for sequencing nucleic acid molecules. Methods may include sequencing double-stranded nucleic acids or single-stranded nucleic acids. Sequencing may include the use of nucleotides coupled to electrostatic moieties. The electrostatic moieties may be detected by a sensor array. The electrostatic moieties may be reversible electrostatic moieties that are cleaved from the nucleic acid molecule after incorporation of the nucleotide. The electrostatic moieties may be irreversible electrostatic moieties. Nucleotides comprising irreversible electrostatic moieties may be incorporated into the nucleic acid molecule, detected by the sensor array, and exchanged for non-detectable nucleotides.

公开(公告)号：US10900075B2

公开(公告)日：2021-01-26

申请号：US16141215

申请日：2018-09-25

Applicant: GenapSys, Inc.

Inventor： Hesaam Esfandyarpour ,  Maryam Jouzi ,  Seth Stern ,  Paul Kenney

IPC: C12Q1/6869 ,  C12Q1/6825 ,  C12Q1/6837

Abstract: The present disclosure provides methods and systems for sequencing nucleic acid molecules. Methods may include sequencing double-stranded nucleic acids or single-stranded nucleic acids. Sequencing may include the use of nucleotides coupled to electrostatic moieties. The electrostatic moieties may be detected by a sensor array. The electrostatic moieties may be reversible electrostatic moieties that are cleaved from the nucleic acid molecule after incorporation of the nucleotide. The electrostatic moieties may be irreversible electrostatic moieties. Nucleotides comprising irreversible electrostatic moieties may be incorporated into the nucleic acid molecule, detected by the sensor array, and exchanged for non-detectable nucleotides.

公开(公告)号：US10544456B2

公开(公告)日：2020-01-28

申请号：US15655616

申请日：2017-07-20

Applicant: GenapSys, Inc.

Inventor： Hesaam Esfandyarpour ,  Kosar Parizi ,  Saurabh Paliwal ,  Seth Stern ,  Paul Kenney ,  Meysam R. Barmi ,  Ali Nabi ,  Hamid Rategh

IPC: C12Q1/6869

Abstract: Provided herein are systems and methods for processing and analyzing nucleic acids and other biomolecules. Methods may include processing nucleic acid molecules in an emulsion of droplets. Methods of analyzing nucleic acid molecules may include coupling nucleic acids to a bead or other support. Methods may include analysis of nucleic acid molecules using a redox mediator. In some cases, analysis of the nucleic acid molecule includes determining a nucleotide sequence of the nucleic acid molecule.

公开(公告)号：US20180100190A1

公开(公告)日：2018-04-12

申请号：US15655616

申请日：2017-07-20

Applicant: GenapSys, Inc.

Inventor： Hesaam Esfandyarpour ,  Aldrich N.K. Lau ,  Kosar Parizi ,  Saurabh Paliwal ,  Henrik Persson ,  Seth Stern ,  Paul Kenney ,  Meysam R. Barmi ,  Ali Nabi ,  Hamid Rategh ,  Florian Oberstrass

IPC: C12Q1/6869

Abstract: Provided herein are systems and methods for processing and analyzing nucleic acids and other biomolecules. Methods may include processing nucleic acid molecules in an emulsion of droplets. Methods of analyzing nucleic acid molecules may include coupling nucleic acids to a bead or other support. Methods may include analysis of nucleic acid molecules using a redox mediator. In some cases, analysis of the nucleic acid molecule includes determining a nucleotide sequence of the nucleic acid molecule.