公开(公告)号：US20220186303A1

公开(公告)日：2022-06-16

申请号：US17383716

申请日：2021-07-23

Applicant: GenapSys, Inc.

Inventor： Hesaam Esfandyarpour ,  Maryam Jouzi ,  Seth Stern ,  Paul Kenney

IPC: C12Q1/6869 ,  C12Q1/6825

Abstract: The present disclosure provides methods and systems for sequencing nucleic acid molecules. Methods may include sequencing double-stranded nucleic acids or single-stranded nucleic acids. Sequencing may include the use of nucleotides coupled to electrostatic moieties. The electrostatic moieties may be detected by a sensor array. The electrostatic moieties may be reversible electrostatic moieties that are cleaved from the nucleic acid molecule after incorporation of the nucleotide. The electrostatic moieties may be irreversible electrostatic moieties. Nucleotides comprising irreversible electrostatic moieties may be incorporated into the nucleic acid molecule, detected by the sensor array, and exchanged for non-detectable nucleotides.

公开(公告)号：US20200255893A1

公开(公告)日：2020-08-13

申请号：US16598591

申请日：2019-10-10

Applicant: GenapSys, Inc.

Inventor： Hesaam Esfandyarpour ,  Kosar Baghbani Parizi ,  Mark F. Oldham ,  Eric S. Nordman ,  Richard T. Reel ,  Susanne Baumhueter ,  Cheryl Heiner ,  Frank Lee

IPC: C12Q1/6874 ,  B01L3/00

Abstract: The invention relate to systems and methods for sequencing polynucleotides, as well as detecting reactions and binding events involving other biological molecules. The systems and methods may employ chamber-free devices and nanosensors to detect or characterize such reactions in high-throughput. Because the system in many embodiments is reusable, the system can be subject to more sophisticated and improved engineering, as compared to single use devices.

公开(公告)号：US10570449B2

公开(公告)日：2020-02-25

申请号：US15726217

申请日：2017-10-05

Applicant: GenapSys, Inc.

Inventor： Hesaam Esfandyarpour ,  Hamid Rategh ,  Meysam R. Barmi ,  Kosar B. Parizi ,  Kambiz Kaviani

IPC: C12Q1/68 ,  C12Q1/6874 ,  G01N27/74

Abstract: Provided herein are devices and methods suitable for sequencing, amplifying, analyzing, and performing sample preparation procedures for nucleic acids and other biomolecules.

公开(公告)号：US10533218B2

公开(公告)日：2020-01-14

申请号：US15726193

申请日：2017-10-05

Applicant: GenapSys, Inc.

Inventor： Florian Oberstrass

IPC: C12Q1/68 ,  C12Q1/6848 ,  C12Q1/6853

Abstract: The disclosure provides methods and systems for nucleic acid amplification including isothermal nucleic acid amplification.

公开(公告)号：US10413900B2

公开(公告)日：2019-09-17

申请号：US15380566

申请日：2016-12-15

Applicant: GenapSys, Inc.

Inventor： Seth Stern ,  Hesaam Esfandyarpour ,  David Eberhart

IPC: G01N35/00 ,  G01N1/00 ,  G01N1/10 ,  B01L3/00 ,  C12N15/10 ,  B01L7/00 ,  B01F5/02 ,  B01F13/00 ,  B01F3/08 ,  F16K99/00 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: The present disclosure provides microfluidic devices, systems and methods for sample preparation and/or analysis. A microfluidic device can include a first channel having a sequence of (n) chambers each having a first volume (v). The first channel can include one or more valves at opposing ends of the first channel that fluidically isolate the first channel. The microfluidic device can further include a second channel in fluid communication with the first channel. The second channel can include at least one second chamber having a total second volume that is at least equal to the total volume of the first channel (n*v). The second channel can include one or more valves at opposing ends of the second channel that fluidically isolate the second channel from the first channel.

公开(公告)号：US20180245150A1

公开(公告)日：2018-08-30

申请号：US15896572

申请日：2018-02-14

Applicant: GenapSys, Inc.

Inventor： Hesaam Esfandyarpour ,  Kosar Baghbani Parizi ,  Mark F. Oldham ,  Eric S. Nordman ,  Richard T. Reel ,  Susanne Baumhueter ,  Cheryl Heiner ,  Frank Lee

IPC: C12Q1/6874

Abstract: The invention relate to systems and methods for sequencing polynucleotides, as well as detecting reactions and binding events involving other biological molecules. The systems and methods may employ chamber-free devices and nanosensors to detect or characterize such reactions in high-throughput. Because the system in many embodiments is reusable, the system can be subject to more sophisticated and improved engineering, as compared to single use devices.

公开(公告)号：US20170096705A1

公开(公告)日：2017-04-06

申请号：US15380566

申请日：2016-12-15

Applicant: GenapSys, Inc.

Inventor： Seth Stern ,  Hesaam Esfandyarpour ,  David Eberhart

IPC: C12Q1/68 ,  B01L7/00 ,  B01L3/00

CPC classification number: B01L3/502738 ,  B01F3/0865 ,  B01F5/0206 ,  B01F13/0059 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L7/525 ,  B01L2200/0668 ,  B01L2200/0684 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2300/18 ,  B01L2300/1822 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0655 ,  B01L2400/082 ,  C12N15/1013 ,  C12Q1/6806 ,  C12Q1/6869 ,  F16K99/0015 ,  F16K99/0059 ,  F16K2099/008 ,  F16K2099/0084

Abstract: The present disclosure provides microfluidic devices, systems and methods for sample preparation and/or analysis. A microfluidic device can include a first channel having a sequence of (n) chambers each having a first volume (v). The first channel can include one or more valves at opposing ends of the first channel that fluidically isolate the first channel. The microfluidic device can further include a second channel in fluid communication with the first channel. The second channel can include at least one second chamber having a total second volume that is at least equal to the total volume of the first channel (n*v). The second channel can include one or more valves at opposing ends of the second channel that fluidically isolate the second channel from the first channel.

公开(公告)号：US20170065977A1

公开(公告)日：2017-03-09

申请号：US15183406

申请日：2016-06-15

Applicant: GenapSys, Inc.

Inventor： Hesaam Esfandyarpour ,  Kosar B. Parizi ,  Mark F. Oldham

IPC: B01L3/00 ,  B01L7/00 ,  B03C5/02 ,  B03C5/00 ,  B03C1/30 ,  C12Q1/68 ,  G01N27/414

CPC classification number: C12Q1/6874 ,  B01L3/502738 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/12 ,  B01L2300/123 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0478 ,  B01L2400/0655 ,  B03C1/286 ,  B03C1/288 ,  B03C1/30 ,  B03C5/005 ,  B03C5/022 ,  B03C5/026 ,  B03C2201/26 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q1/6869 ,  C40B60/10 ,  G01N1/40 ,  G01N27/4145 ,  G01N27/4146 ,  G01N33/5438 ,  G01N2001/4038 ,  H01L29/00 ,  C12Q2523/307 ,  C12Q2563/143 ,  C12Q2565/543 ,  C12Q2563/116 ,  C12Q2565/607

Abstract: Aspects of the invention include methods for improving the accuracy and read length of sequencing reactions by utilizing unlabeled unincorporable nucleotides, or by rephasing colony based sequencing reactions. Other aspects include systems and devices for improved measurement of biological reactions associated with bead which may be removed, utilizing current measurement methods through the counter ions associated with said beads due to the presence of reactants bound or associated with said bead, wherein electrodes for generating and measuring said current may be within the Debye length of said bead. Other aspects of the invention include methods for determining concentrations of input samples, means for reuse of an array, methods and apparatus for separating beads with different charge levels from each other.

Abstract translation: 本发明的方面包括通过利用未标记的非可合并核苷酸或通过重新构建基于集落的测序反应来提高测序反应的准确性和读取长度的方法。 其他方面包括用于改进与珠粒相关的生物反应测量的系统和装置，其可以通过与所述珠粒相关联的抗衡离子的电流测量方法，由于存在与所述珠粒结合或相关联的反应物，其中用于产生和 测量所述电流可以在所述焊道的德拜长度内。 本发明的其它方面包括用于确定输入样品的浓度的方法，用于重复使用阵列的装置，用于分离具有不同电荷水平的珠子的方法和装置。

公开(公告)号：US20150376692A1

公开(公告)日：2015-12-31

申请号：US14653230

申请日：2014-03-14

Applicant: GENAPSYS, INC.

Inventor： Hesaam ESFANDYARPOUR ,  Hamid RATEGH ,  Meysam R. BARMI ,  Kosar B. PARIZI ,  Kambiz KAVIANI

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  G01N27/745 ,  C12Q2563/116 ,  C12Q2563/143 ,  C12Q2563/149 ,  C12Q2565/607

Abstract: Provided herein are devices and methods suitable for sequencing, amplifying, analyzing, and performing sample preparation procedures for nucleic acids and other biomolecules.

Abstract translation: 本文提供适用于对核酸和其他生物分子进行测序，扩增，分析和执行样品制备程序的装置和方法。

公开(公告)号：US11286522B2

公开(公告)日：2022-03-29

申请号：US16105480

申请日：2018-08-20

Applicant: GENAPSYS, INC.

Inventor： Hesaam Esfandyarpour

IPC: C12Q1/68 ,  C12Q1/6874 ,  C12Q1/6825 ,  B01L3/00 ,  B03C1/28 ,  B03C5/00 ,  B03C5/02 ,  G01N27/327 ,  C12Q1/6844 ,  C12Q1/6869

Abstract: The present disclosure relates to systems and methods for high efficiency electronic sequencing of nucleic acids and molecular detection.