公开(公告)号：US20170023409A1

公开(公告)日：2017-01-26

申请号：US15120792

申请日：2015-01-16

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Tsuyoshi SONEHARA ,  Hiromi KUSAKA ,  Akira FUJII ,  Shuhei YAMAMOTO ,  Takeshi OOURA ,  Michiru FUJIOKA

IPC: G01J3/44 ,  G01N21/65 ,  G02B21/16 ,  G02B21/24 ,  G02B21/36 ,  G02B27/14 ,  G01J3/02 ,  G02B21/00

CPC classification number: G01J3/44 ,  G01J3/0208 ,  G01J3/021 ,  G01J3/0229 ,  G01J3/0248 ,  G01J3/10 ,  G01J3/4406 ,  G01N21/6458 ,  G01N21/65 ,  G02B7/28 ,  G02B7/32 ,  G02B21/0004 ,  G02B21/16 ,  G02B21/244 ,  G02B21/36 ,  G02B21/365 ,  G02B27/141

Abstract: With a microspectroscopy device provided with an objective lens with a high numerical aperture, a defocus arises from thermal drift, etc., necessitating auto-focusing. Conventional auto-focus based on through-focus image acquisition takes time, and thus, it cannot be applied to continuous measurement over a long time wherein high-speed sampling is carried out. The present invention addresses this problem by having a defocus-sensing beam that has either defocus or astigmatism fall incident on the objective lens. Since how the image of the spot of the beam for defocus sensing blurs differs depending on the orientation of the defocus, real-time detection of the amount and orientation of defocus becomes possible, and high-speed realtime auto-focus becomes possible.

Abstract translation: 利用具有高数值孔径的物镜的显微光谱装置，由于热漂移等引起散焦，需要自动聚焦。 基于通过聚焦图像获取的常规自动对焦需要时间，因此不能将其应用于长时间进行高速采样的连续测量。 本发明通过具有散焦或像散落在物镜上的散焦感测光束来解决这个问题。 由于用于散焦感测模糊的光束的斑点的图像根据散焦的取向而不同，所以可以实现对散焦的量和取向的实时检测，并且可以实现高速实时自动对焦。

公开(公告)号：US20170045726A1

公开(公告)日：2017-02-16

申请号：US15305226

申请日：2015-03-12

Applicant: Hitachi High-Technologies Corporation

Inventor： Michiru FUJIOKA ,  Shuhei YAMAMOTO ,  Tsuyoshi SONEHARA ,  Hiromi KUSAKA ,  Akimasa OSAKA

IPC: G02B21/33 ,  G02B21/08 ,  G02B21/12 ,  G02B21/26

CPC classification number: G02B21/33 ,  G02B21/082 ,  G02B21/12 ,  G02B21/26 ,  G02B21/34

Abstract: In the microscope observation container according to the present invention, an observed sample is accommodated by an objective lens barrel provided with a housing extending along the radiation direction of excitation light and an objective lens fixed to an inside surface of the housing. The microscope observation container is provided with a structure for collecting a liquid immersion medium added by dispensation, the structure having a portion contacted by the objective lens barrel during observation. During observation the aforementioned portion is contacted by the objective lens barrel, and the liquid immersion medium is thereby sealed by the objective lens barrel and the structure. The aforementioned portion also has an elastic force, and is deformed so as to conform to the housing of the objective lens by the contact.

Abstract translation: 在根据本发明的显微镜观察容器中，通过设置有沿着激发光的辐射方向延伸的壳体的物镜筒和固定到壳体的内表面的物镜容纳观察到的样品。 显微镜观察容器设置有用于收集通过分配而添加的浸液介质的结构，该结构具有在观察期间与物镜镜筒接触的部分。 在观察期间，上述部分被物镜筒接触，并且液浸介质由此被物镜镜筒和结构密封。 上述部分还具有弹性力，并且通过接触而变形以与物镜的壳体一致。

公开(公告)号：US20150024401A1

公开(公告)日：2015-01-22

申请号：US14508338

申请日：2014-10-07

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Minoru SANO ,  Masato ISHIZAWA ,  Shuhei YAMAMOTO

IPC: C12Q1/68

CPC classification number: C12Q1/68 ,  B01L7/52 ,  G01N21/0332 ,  G01N21/645 ,  G01N21/6452 ,  G01N35/025 ,  G01N2035/00366

Abstract: An object of the present invention relates to providing a nucleic acid analyzer capable of testing a plurality of test items in parallel, and of obtaining high efficiency of specimen processing even if the test item or a measuring object is changed. The present invention relates to an analyzer including a carousel rotatable about a rotation axis, a plurality of reaction containers held along a circumferential edge of the carousel, and at least one detector having a light source for irradiating the reaction container with excitation light and a detection element for detecting fluorescence from a reaction liquid in the reaction container. The detector is removable. By attaching a desired detector, it is possible to perform fluorescence measurement in response to the test item. According to the present invention, it is possible to test a plurality of test items in parallel, and even if the test item or the measuring object is changed, the high efficiency of specimen processing can be obtained.

Abstract translation: 本发明的目的是提供一种能够并行地测试多个测试项目的核酸分析仪，并且即使测试项目或测量对象改变也能获得高效率的样本处理。 本发明涉及一种分析仪，其包括可围绕旋转轴线旋转的圆盘传送带，沿圆盘传送带的圆周边缘保持的多个反应容器，以及至少一个检测器，具有用于向反应容器照射激发光和检测光的源 用于检测来自反应容器中的反应液体的荧光的元件。 检测器是可拆卸的。 通过附加所需的检测器，可以响应于测试项目执行荧光测量。 根据本发明，可以并行地进行多个测试项目的测试，即使测试项目或测量对象发生变化，也能够获得高效的样本处理。