公开(公告)号：US20230175886A1

公开(公告)日：2023-06-08

申请号：US17920054

申请日：2021-04-23

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Holger Birk ,  Frank Hecht ,  Luis Alvarez ,  Bernd Widzgowski

IPC: G01J1/44 ,  G01N21/64 ,  G02B21/00

CPC classification number: G01J1/44 ,  G01N21/6408 ,  G01N21/6458 ,  G02B21/0076 ,  G02B21/0084 ,  G01J2001/442

Abstract: An apparatus is configured to count N-photon events within a time-dependent sequence of events of interactions of a plurality of photons with a light sensitive detector. The apparatus includes a signal-processing device and the light sensitive detector. An N-photon event represents an occurrence of at least N timely overlapping single photon events. The light sensitive detector is adapted to generate a time-dependent digital signal comprising digital patterns representing the time-dependent sequence of events from the detection of the plurality of photons with the light sensitive detector. Each digital pattern in the digital signal comprises a digital pattern width having a continuous sequence of digital values representing at least one event of interaction of at least one photon with the light sensitive detector. The signal-processing device is adapted to identify N-photon events from the digital patterns in the digital signal in dependence from the respective digital pattern width.

公开(公告)号：US10261306B2

公开(公告)日：2019-04-16

申请号：US14398513

申请日：2013-04-19

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Frank Hecht ,  Frank Sieckmann

IPC: G02B21/00 ,  G02B21/36 ,  G06F3/0481 ,  G06F3/0484 ,  G06F3/0488

Abstract: A method for execution upon operation of a microscope or for depiction of an object, or a part thereof, imaged with the microscope includes depicting a proxy of the object on a display of the microscope or on a further display. At least one manipulation is performed on the proxy, or on the depiction of the proxy, using an input means. At least one depiction parameter for the depiction of the object or of the part of the object, or at least one microscope control parameter, is derived from the manipulation. The object or the part of the object is depicted in consideration of the derived depiction parameter or of the derived microscope control parameter.

公开(公告)号：US12243194B2

公开(公告)日：2025-03-04

申请号：US17636035

申请日：2020-10-08

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Luis Alvarez ,  Frank Hecht

IPC: G06T5/70 ,  G01N21/64 ,  G06T5/10 ,  G06T5/20 ,  G06T5/50

Abstract: The present invention relates to a method for generating a result image. The method comprises the steps of: acquiring a STED image of a sample, the STED image comprising pixels; calculating Fourier coefficients of arrival times for the pixels of the image, resulting in real coefficients representing a first image and in imaginary coefficients representing a second image; deriving an intensity image from the STED image; applying a spatial filter to the first image, the second image and the intensity image, resulting in a filtered first image, a filtered second image, and filtered intensity image, respectively; calculating an image G based on the filtered first image and the filtered intensity image; calculating an image S based on the filtered second image and the filtered intensity image; and calculating a result image based on the image G and the image S.

公开(公告)号：US12278654B2

公开(公告)日：2025-04-15

申请号：US18026644

申请日：2021-09-20

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Frank Hecht

IPC: H03M7/00 ,  H03M7/30

Abstract: A computer-implemented method for compression of Time Tagged data including Time Tagged data records includes the step of separating the Time Tagged data records into a plurality of groups. The method also includes sorting the Time Tagged data records in at least one of the groups by a photon arrival time. The method also includes subtracting a content of a record by a content of an adjacent record resulting in modified records. The method also includes compressing the modified records with a compression method.

公开(公告)号：US11086119B2

公开(公告)日：2021-08-10

申请号：US16969645

申请日：2018-12-18

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Frank Hecht ,  Bernd Widzgowski

IPC: G02B21/06 ,  G02B21/36 ,  G01N21/64 ,  G02B21/16

Abstract: A fluorescence-lifetime imaging microscopy method with time-correlated single-photon counting includes using excitation light pulses separated in each case by a measurement interval to excite a sample to emit fluorescence photons. A detector signal that represents the captured fluorescence photons is generated. Detection times are determined based on the detector signal. Imaging is performed based on the detection times. The detection times of all captured fluorescence photons are compiled in a first data memory, common to a plurality of image pixels. The detection times of only those fluorescence photons which were captured in a predetermined number within the respective measurement intervals are compiled in a second data memory, common to the same plurality of image pixels. The detection times compiled in the data memories are combined within a calculation step. The results of the calculation step are stored in a third data memory.

公开(公告)号：US20150143274A1

公开(公告)日：2015-05-21

申请号：US14398513

申请日：2013-04-19

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Frank Hecht ,  Frank Sieckmann

IPC: G02B21/36 ,  G02B21/00 ,  G06F3/0488 ,  G06F3/0481 ,  G06F3/0484

CPC classification number: G02B21/367 ,  G02B21/008 ,  G02B21/36 ,  G06F3/04815 ,  G06F3/04845 ,  G06F3/04847 ,  G06F3/04883

Abstract: A method for execution upon operation of a microscope or for depiction of an object, or a part thereof, imaged with the microscope includes depicting a proxy of the object on a display of the microscope or on a further display. At least one manipulation is performed on the proxy, or on the depiction of the proxy, using an input means. At least one depiction parameter for the depiction of the object or of the part of the object, or at least one microscope control parameter, is derived from the manipulation. The object or the part of the object is depicted in consideration of the derived depiction parameter or of the derived microscope control parameter.

Abstract translation: 用显微镜操作或用于用显微镜成像的物体或其部分的描绘时执行的方法包括在显微镜的显示器上或在另外的显示器上描绘物体的代理。 使用输入装置对代理进行至少一次操作，或对代理进行描述。 从操作中导出至少一个用于描绘对象或部分对象的描绘参数，或至少一个显微镜控制参数。 考虑到衍生的描绘参数或衍生的显微镜控制参数来描绘物体或物体的一部分。

公开(公告)号：US10520434B2

公开(公告)日：2019-12-31

申请号：US16304168

申请日：2017-05-24

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Volker Seyfried ,  Bernd Widzgowski ,  Frank Hecht

IPC: G01N21/64

Abstract: A fluorescence lifetime imaging microscopy method with time-correlated single photon counting includes periodically exciting a sample to emit fluorescence photons, with a measurement interval being defined between each two successive excitation light pulses. A value characterizing fluorescence decay behavior is determined based on detection times of detected fluorescence photons, and imaging is performed based one the value. An analog detector signal is sampled within a plurality of sampling intervals within a respective one of the measurement intervals and is converted into a sequence of discrete signal values associated with the sampling intervals. It is determined based thereon whether more than a predefined number of fluorescence photons has been detected within the respective measurement interval. If more than the predefined number of fluorescence photons has been detected, the respective measurement interval is discarded in the step of determining the value characterizing the fluorescence decay behavior.