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公开(公告)号：US11686994B2
公开(公告)日：2023-06-27
申请号：US16711483
申请日：2019-12-12
Applicant: Leica Microsystems CMS GmbH
Inventor： Bernd Widzgowski
CPC classification number: G02F1/33 , G02B21/0076 , G02F1/11 , G02F1/113 , H01S3/0057
Abstract: A method for driving an acousto-optic element with an acousto-optic crystal and a piezoelectric transducer for setting the acousto-optic crystal in mechanical vibration includes driving the piezoelectric transducer with a drive signal with at least one drive frequency. The at least one drive frequency in alternation takes on a plurality of different values around a center frequency during a passage of a mechanical vibrational wave through the acousto-optic crystal, such that a grating that is produced owing to density fluctuations in the acousto-optic crystal exhibits different grating spacings at the same time.
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公开(公告)号：US20190041671A1
公开(公告)日：2019-02-07
申请号：US16053837
申请日：2018-08-03
Applicant: Leica Microsystems CMS GmbH
Inventor： Bernd Widzgowski , Patric Mrawek
IPC: G02F1/11 , H01L41/09 , H01L41/04 , H01L41/107
Abstract: An electric circuit for driving an acousto-optic crystal includes a piezoelectric converter configured to drive the acousto-optic crystal to vibrate mechanically. A signaling cable is configured to conduct a first electrical alternating-current signal and a second electrical signal. The electric circuit further includes a first frequency-separating filter and a second frequency-separating filter, each of the frequency-separating filters having an input, a high-frequency output and a low-frequency output. The input of the first frequency-separating filter and the input of the second frequency-separating filter is connected to the signaling cable, and the high-frequency output of the second frequency-separating filter is connected to the piezoelectric converter.
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公开(公告)号：US09411141B2
公开(公告)日：2016-08-09
申请号：US14436919
申请日：2013-09-27
Applicant: LEICA MICROSYSTEMS CMS GMBH
Inventor： Frank Schreiber , Lioba Kuschel , Patric Mrawek , Bernd Widzgowski
CPC classification number: G02B21/0064 , G01N21/6486 , G01N2201/0612 , G02B21/0076 , G02B21/008 , G02B21/0084 , G02B21/16 , G02B21/365
Abstract: A microscope, in particular a confocal microscope, has one or more lasers for generating an illumination light for a sample and has a detection device for detected signals from the sample. The detection device includes multiple adjustable spectral detection channels for the detection of predefinable different wavelength regions, and is configured and refined in the interest of particularly versatile utilization in consideration of a wide variety of phenomena, with particularly good separation of the phenomena in the context of investigation, in such a way that multiple temporal detection windows are respectively settable for the spectral detection channels.
Abstract translation: 显微镜，特别是共聚焦显微镜，具有用于产生样品的照明光的一个或多个激光器，并且具有用于来自样品的检测信号的检测装置。 该检测装置包括用于检测预定义的不同波长区域的多个可调节的光谱检测通道，并且考虑到各种各样的现象，特别是在多种多样的利用方面被配置和改进，特别是在 调查，使得多个时间检测窗口分别可设置用于频谱检测通道。
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4.发明授权公开(公告)号：US09274057B2
公开(公告)日：2016-03-01
申请号：US14401952
申请日：2013-04-19
Applicant: Leica Microsystems CMS GmbH
Inventor： Juergen Schneider , Bernd Widzgowski , Jochen Sieber , Wernher Fouquet , Lars Friedrich , Arnold Giske , Lioba Kuschel
CPC classification number: G01N21/6428 , G01N21/6408 , G01N21/6458 , G01N2201/06113 , G02B21/0076
Abstract: The invention relates to a method for investigating a sample with regard to the lifetime of an excited state, in particular a fluorescence lifetime, and/or with regard to a property of a sample which is correlated with a lifetime of an excited state, in particular with a fluorescence lifetime, a sample region being illuminated with a sequence of excitation light pulses. The method is characterized in that the light quantity and/or number of photons of the detected light, in particular fluorescent light, proceeding from the sample region is measured temporally between the excitation light pulses exclusively within a detection time window in each case, at least two detection time windows having different temporal lengths.
Abstract translation: 本发明涉及一种关于激发态的寿命，特别是荧光寿命和/或关于与激发态的寿命相关的样品的性质的方法，特别是 具有荧光寿命，用一系列激发光脉冲照射样品区域。 该方法的特征在于，在每种情况下，在检测时间窗内唯一地在激发光脉冲之间时间上测量从样本区域进行的检测光，特别是荧光的光子的光量和/或数量，至少 两个具有不同时间长度的检测时间窗口。
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公开(公告)号：US20150286040A1
公开(公告)日：2015-10-08
申请号：US14436919
申请日：2013-09-27
Applicant: LEICA MICROSYSTEMS CMS GMBH
Inventor： Frank Schreiber , Lioba Kuschel , Patric Mrawek , Bernd Widzgowski
CPC classification number: G02B21/0064 , G01N21/6486 , G01N2201/0612 , G02B21/0076 , G02B21/008 , G02B21/0084 , G02B21/16 , G02B21/365
Abstract: A microscope, in particular a confocal microscope, has one or more lasers for generating an illumination light for a sample and has a detection device for detected signals from the sample. The detection device includes multiple adjustable spectral detection channels for the detection of predefinable different wavelength regions, and is configured and refined in the interest of particularly versatile utilization in consideration of a wide variety of phenomena, with particularly good separation of the phenomena in the context of investigation, in such a way that multiple temporal detection windows are respectively settable for the spectral detection channels.
Abstract translation: 显微镜，特别是共聚焦显微镜，具有用于产生样品的照明光的一个或多个激光器，并且具有用于来自样品的检测信号的检测装置。 该检测装置包括用于检测预定义的不同波长区域的多个可调节的光谱检测通道，并且考虑到各种各样的现象，特别是在多种多样的利用方面被配置和改进，特别是在 调查，使得多个时间检测窗口分别可设置用于频谱检测通道。
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Patent Agency Ranking
6.发明申请CONFOCAL LASER SCANNING MICROSCOPE HAVING A LASER LIGHT SOURCE TO WHICH PULSED CONTROL IS APPLIED 有权具有脉冲控制的激光光源的共焦激光扫描显微镜应用公开(公告)号：US20150212306A1
公开(公告)日：2015-07-30
申请号：US14426260
申请日：2013-09-04
Applicant: Leica Microsystems CMS GmbH
Inventor： Bernd Widzgowski
IPC: G02B21/00
CPC classification number: G02B21/0032 , G02B21/0096
Abstract: The present invention relates to a confocal laser scanning microscope (100) having an illumination device (1) that comprises a laser light source (41) that is configured to illuminate a sample (25), and a control application circuit (40) for the laser light source (1) which is configured to output a pulsed control application signal (48) in order to supply the laser light source (41), the control application circuit (40) being configured so that it determines both a pulse amplitude (A) and a pulse width (W) of at least one pulse of the pulsed control application signal (48) as a function of at least one input variable (S).
Abstract translation: 本发明涉及具有照明装置（1）的共焦激光扫描显微镜（100），所述照明装置（1）包括被配置为照射样品（25）的激光光源（41），以及用于 激光光源（1），被配置为输出脉冲控制应用信号（48）以提供所述激光源（41），所述控制应用电路（40）被配置为使得其确定脉冲幅度（A ）和作为至少一个输入变量（S）的函数的脉冲控制应用信号（48）的至少一个脉冲的脉冲宽度（W）。
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公开(公告)号：US11215503B2
公开(公告)日：2022-01-04
申请号：US16641674
申请日：2018-08-28
Applicant: Leica Microsystems CMS GmbH
Inventor： Holger Birk , Bernd Widzgowski
Abstract: A method for counting photons using a photomultiplier includes obtaining a measurement signal from a raw signal produced by the photomultiplier by correcting the raw signal for a noise signal and/or an offset, wherein an incident photon produces a pulse in the raw signal. The measurement signal is integrated over time to form an analog integrated measurement signal. A number of photons that are incident in the photomultiplier is ascertained by comparing a value of the analog integrated measurement signal to an integral proportionality value which corresponds to a specific number of photons incident in the photomultiplier.
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8.发明授权Confocal laser scanning microscope having a laser light source to which pulsed control is applied 有权具有应用脉冲控制的激光光源的共焦激光扫描显微镜公开(公告)号：US09575300B2
公开(公告)日：2017-02-21
申请号：US14426260
申请日：2013-09-04
Applicant: Leica Microsystems CMS GmbH
Inventor： Bernd Widzgowski
IPC: G02B21/00
CPC classification number: G02B21/0032 , G02B21/0096
Abstract: The present invention relates to a confocal laser scanning microscope (100) having an illumination device (1) that comprises a laser light source (41) that is configured to illuminate a sample (25), and a control application circuit (40) for the laser light source (1) which is configured to output a pulsed control application signal (48) in order to supply the laser light source (41), the control application circuit (40) being configured so that it determines both a pulse amplitude (A) and a pulse width (W) of at least one pulse of the pulsed control application signal (48) as a function of at least one input variable (S).
Abstract translation: 本发明涉及具有照明装置（1）的共焦激光扫描显微镜（100），所述照明装置（1）包括被配置为照射样品（25）的激光光源（41），以及用于 激光光源（1），被配置为输出脉冲控制应用信号（48）以提供所述激光源（41），所述控制应用电路（40）被配置为使得其确定脉冲幅度（A ）和作为至少一个输入变量（S）的函数的脉冲控制应用信号（48）的至少一个脉冲的脉冲宽度（W）。
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公开(公告)号：US11215853B2
公开(公告)日：2022-01-04
申请号：US16053837
申请日：2018-08-03
Applicant: Leica Microsystems CMS GmbH
Inventor： Bernd Widzgowski , Patric Mrawek
Abstract: An electric circuit for driving an acousto-optic crystal includes a piezoelectric converter configured to drive the acousto-optic crystal to vibrate mechanically. A signaling cable is configured to conduct a first electrical alternating-current signal and a second electrical signal. The electric circuit further includes a first frequency-separating filter and a second frequency-separating filter, each of the frequency-separating filters having an input, a high-frequency output and a low-frequency output. The input of the first frequency-separating filter and the input of the second frequency-separating filter is connected to the signaling cable, and the high-frequency output of the second frequency-separating filter is connected to the piezoelectric converter.
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10.发明授权公开(公告)号：US11086119B2
公开(公告)日：2021-08-10
申请号：US16969645
申请日：2018-12-18
Applicant: LEICA MICROSYSTEMS CMS GMBH
Inventor： Frank Hecht , Bernd Widzgowski
Abstract: A fluorescence-lifetime imaging microscopy method with time-correlated single-photon counting includes using excitation light pulses separated in each case by a measurement interval to excite a sample to emit fluorescence photons. A detector signal that represents the captured fluorescence photons is generated. Detection times are determined based on the detector signal. Imaging is performed based on the detection times. The detection times of all captured fluorescence photons are compiled in a first data memory, common to a plurality of image pixels. The detection times of only those fluorescence photons which were captured in a predetermined number within the respective measurement intervals are compiled in a second data memory, common to the same plurality of image pixels. The detection times compiled in the data memories are combined within a calculation step. The results of the calculation step are stored in a third data memory.
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