公开(公告)号：US11198878B2

公开(公告)日：2021-12-14

申请号：US17006675

申请日：2020-08-28

Applicant: Labcyte Inc.

Inventor： Jennifer M. Hardee ,  Richard N. Ellson ,  Richard G. Stearns ,  Babur Hadimioglu ,  Joseph D. Olechno ,  Marsha N. Blauwkamp

IPC: C07H21/04 ,  C12N15/64 ,  C12N15/87 ,  A61N7/00 ,  C12N9/22 ,  C12N13/00 ,  C12N15/11

Abstract: A method is provided for achieving transfection of host cells using sonoporation. An acoustic radiation generator is positioned in acoustic coupling relationship with respect to a reservoir containing host cells to be transfected, exogenous material to be incorporated into the host cells, and a cell-compatible fluid medium. The acoustic radiation generator is activated to generate acoustic radiation and direct the acoustic radiation into the reservoir in a manner effective to enable transfection of the host cells with the exogenous material.

公开(公告)号：US20190302063A1

公开(公告)日：2019-10-03

申请号：US16369780

申请日：2019-03-29

Applicant: Labcyte Inc.

Inventor： Babur Hadimioglu ,  Jay Daulton ,  Richard N. Ellson

IPC: G01N29/32 ,  H04R23/00 ,  H04R31/00 ,  G01N29/28 ,  G01N29/24

Abstract: A fluid impermeable transducer includes an assembly of a transducer head and a casing, and an actuator disposed in the casing rearward of the back of the transducer head and operable to transmit acoustic energy through the transducer head. The transducer head and casing define a working portion of the transducer that is fluid impermeable.

公开(公告)号：US20180073029A1

公开(公告)日：2018-03-15

申请号：US15706524

申请日：2017-09-15

Applicant: Labcyte Inc.

Inventor： Jennifer M. Hardee ,  Richard N. Ellson ,  Richard G. Stearns ,  Babur Hadimioglu ,  Joseph D. Olechno ,  Marsha N. Blauwkamp

IPC: C12N15/64 ,  C12N13/00 ,  C12N15/11 ,  C12N9/22

Abstract: A method is provided for achieving transfection of host cells using sonoporation. An acoustic radiation generator is positioned in acoustic coupling relationship with respect to a reservoir containing host cells to be transfected, exogenous material to be incorporated into the host cells, and a cell-compatible fluid medium. The acoustic radiation generator is activated to generate acoustic radiation and direct the acoustic radiation into the reservoir in a manner effective to enable transfection of the host cells with the exogenous material.

公开(公告)号：US10787670B2

公开(公告)日：2020-09-29

申请号：US15706524

申请日：2017-09-15

Applicant: Labcyte Inc.

Inventor： Jennifer M. Hardee ,  Richard N. Ellson ,  Richard G. Stearns ,  Babur Hadimioglu ,  Joseph D. Olechno ,  Marsha N. Blauwkamp

IPC: C07H21/04 ,  C12N15/64 ,  C12N15/87 ,  A61N7/00 ,  C12N9/22 ,  C12N13/00 ,  C12N15/11

Abstract: A method is provided for achieving transfection of host cells using sonoporation. An acoustic radiation generator is positioned in acoustic coupling relationship with respect to a reservoir containing host cells to be transfected, exogenous material to be incorporated into the host cells, and a cell-compatible fluid medium. The acoustic radiation generator is activated to generate acoustic radiation and direct the acoustic radiation into the reservoir in a manner effective to enable transfection of the host cells with the exogenous material.

公开(公告)号：US20220088617A1

公开(公告)日：2022-03-24

申请号：US17424198

申请日：2020-01-31

Applicant: Labcyte Inc.

Inventor： Richard N. Ellson ,  Richard G. Stearns ,  Babur Hadimioglu

IPC: B05B1/08 ,  C12N13/00 ,  B05B17/06 ,  G01N15/10

Abstract: Systems and methods described herein employ focused acoustic energy applied to a reservoir containing a fluid to eject a fluid sample from the fluid sample reservoir, e.g. to an inlet of an analytical device. In many embodiments, the ejected fluid sample traverses an air gap separating the inlet of the analytical device from an upper surface of the fluid in the fluid sample reservoir. In many embodiments, the ejected fluid sample comprises one or more droplets ejected from the fluid sample reservoir, which can contain particles suspended in the fluid sample.

公开(公告)号：US20220025382A1

公开(公告)日：2022-01-27

申请号：US17497792

申请日：2021-10-08

Applicant: Labcyte Inc.

Inventor： Jennifer M. Hardee ,  Richard N. Ellson ,  Richard G. Stearns ,  Babur Hadimioglu ,  Joseph D. Olechno ,  Marsha N. Blauwkamp

IPC: C12N15/64 ,  C12N15/87 ,  A61N7/00 ,  C12N9/22 ,  C12N13/00 ,  C12N15/11

Abstract: A method is provided for achieving transfection of host cells using sonoporation. An acoustic radiation generator is positioned in acoustic coupling relationship with respect to a reservoir containing host cells to be transfected, exogenous material to be incorporated into the host cells, and a cell-compatible fluid medium. The acoustic radiation generator is activated to generate acoustic radiation and direct the acoustic radiation into the reservoir in a manner effective to enable transfection of the host cells with the exogenous material.

公开(公告)号：US11898993B2

公开(公告)日：2024-02-13

申请号：US16369780

申请日：2019-03-29

Applicant: Labcyte Inc.

Inventor： Babur Hadimioglu ,  Jay Daulton ,  Richard N. Ellson

IPC: G01N29/32 ,  G01N29/24 ,  G01N29/28 ,  H04R23/00 ,  H04R31/00 ,  B06B1/06 ,  G10K11/30 ,  G10K11/02

CPC classification number: G01N29/32 ,  B06B1/067 ,  B06B1/0685 ,  G01N29/2437 ,  G01N29/2456 ,  G01N29/28 ,  G10K11/02 ,  G10K11/30 ,  H04R23/00 ,  H04R31/00

Abstract: A fluid impermeable transducer includes an assembly of a transducer head and a casing, and an actuator disposed in the casing rearward of the back of the transducer head and operable to transmit acoustic energy through the transducer head. The transducer head and casing define a working portion of the transducer that is fluid impermeable.