公开(公告)号：US20210346866A1

公开(公告)日：2021-11-11

申请号：US17376567

申请日：2021-07-15

Applicant: Puridify Ltd.

Inventor： Marc Hummersone ,  Benjamin Wallis ,  Frederic Leroux ,  William Law

IPC: B01J20/26 ,  B01D15/20 ,  B01D15/38 ,  B01J20/28 ,  B01J20/289 ,  B01J20/30 ,  B01J20/32 ,  C07K1/16 ,  B01J20/286

Abstract: Functionalised polymeric chromatography medium, comprising: at least one non-woven sheet comprising one or more polymeric nanofibers having a mean diameter of 10-1000 nm; one or more polymer chains grafted onto the one or more polymeric nanofibers, wherein the polymer chains are poly-glycerol chains comprising glycidol monomer residues or wherein the polymer chains comprise divinylsulfone monomer residues; and at least one ligand group bonded to the one or more polymer chains.

公开(公告)号：US20190308169A1

公开(公告)日：2019-10-10

申请号：US16315088

申请日：2017-07-14

Applicant: Puridify Ltd.

Inventor： Marc Hummersone ,  Benjamin Wallis ,  Frederic Leroux ,  William Law

IPC: B01J20/26 ,  B01J20/28 ,  B01J20/289 ,  B01J20/30 ,  B01J20/32 ,  C07K1/16 ,  B01D15/20 ,  B01D15/38

Abstract: The present invention provides a functionalised polymeric chromatography medium, prepared by a process which comprises (i) providing a substrate formed of one or more polymer nanofibres, (ii) grafting one or more neutral polymer chains from the substrate, and (iii) contacting the grafted product with a reagent which functionalises the product of step (ii) as a chromatography medium, wherein step (ii) comprises reacting a plurality of compounds of formula and/or its enantiomers, and/or its derivatives of formula (I) and/or enantiomers and/or diastereomers thereof: with one or more functional groups present on the nanofibre substrate, wherein R1, R2, R3, R4 and R5 may be the same or different, and are chosen from H, halogen, C1-C4 alkyl, or C1-C4 alkoxy provided that at least one of R1, R2, R3, R4 or R5 is not hydrogen.

公开(公告)号：US11998890B2

公开(公告)日：2024-06-04

申请号：US17376567

申请日：2021-07-15

Applicant: Puridify Ltd.

Inventor： Marc Hummersone ,  Benjamin Wallis ,  Frederic Leroux ,  William Law

IPC: B01D15/20 ,  B01D15/38 ,  B01J20/26 ,  B01J20/28 ,  B01J20/286 ,  B01J20/289 ,  B01J20/30 ,  B01J20/32 ,  C07K1/16

CPC classification number: B01J20/265 ,  B01D15/20 ,  B01D15/3809 ,  B01J20/28023 ,  B01J20/28038 ,  B01J20/28052 ,  B01J20/286 ,  B01J20/289 ,  B01J20/3085 ,  B01J20/3092 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/3217 ,  B01J20/3219 ,  B01J20/3227 ,  B01J20/3248 ,  B01J20/3272 ,  B01J20/328 ,  B01J20/3293 ,  C07K1/16 ,  B01J2220/62

Abstract: Functionalised polymeric chromatography medium, comprising: at least one non-woven sheet comprising one or more polymeric nanofibers having a mean diameter of 10-1000 nm; one or more polymer chains grafted onto the one or more polymeric nanofibers, wherein the polymer chains are poly-glycerol chains comprising glycidol monomer residues or wherein the polymer chains comprise divinylsulfone monomer residues; and at least one ligand group bonded to the one or more polymer chains.

公开(公告)号：US11931719B2

公开(公告)日：2024-03-19

申请号：US16959373

申请日：2019-01-07

Applicant: Puridify Ltd.

Inventor： Marc Hummersone ,  Benjamin Wallis ,  Frederic Leroux ,  William Howard Albert Law

IPC: B01D15/32 ,  B01D15/36 ,  B01J20/26 ,  B01J20/285 ,  B01J20/32 ,  B01J39/05 ,  B01J39/07 ,  B01J41/05 ,  B01J41/07

CPC classification number: B01J20/265 ,  B01D15/327 ,  B01D15/362 ,  B01D15/363 ,  B01J20/285 ,  B01J20/3274 ,  B01J39/05 ,  B01J39/07 ,  B01J41/05 ,  B01J41/07 ,  B01J2219/00844 ,  B01J2219/00846

Abstract: The invention discloses a functionalised chromatography medium, comprising: i) at least one non-woven layer (10) of polymeric nano fibres (20) comprising a plurality of nanofibre-nano fibre fusion points (30); ii) a grafted polymer coating covering the polymeric nanofibres and the nanofibre-nanofibre fusion points; iii) a plurality of ligand groups covalently bound to the grafted polymer coating, wherein the ligand groups are capable of interacting with a target biomolecule.

公开(公告)号：US11097252B2

公开(公告)日：2021-08-24

申请号：US16315088

申请日：2017-07-14

Applicant: Puridify Ltd.

Inventor： Marc Hummersone ,  Benjamin Wallis ,  Frederic Leroux ,  William Law

IPC: B01D15/20 ,  B01D15/38 ,  B01J20/26 ,  B01J20/28 ,  B01J20/286 ,  B01J20/289 ,  B01J20/30 ,  B01J20/32 ,  C07K1/16

Abstract: The present invention provides a functionalised polymeric chromatography medium, prepared by a process which comprises (i) providing a substrate formed of one or more polymer nanofibres, (ii) grafting one or more neutral polymer chains from the substrate, and (iii) contacting the grafted product with a reagent which functionalises the product of step (ii) as a chromatography medium, wherein step (ii) comprises reacting a plurality of compounds of formula and/or its enantiomers, and/or its derivatives of formula (I) and/or enantiomers and/or diastereomers thereof: with one or more functional groups present on the nanofibre substrate, wherein R1, R2, R3, R4 and R5 may be the same or different, and are chosen from H, halogen, C1-C4 alkyl, or C1-C4 alkoxy provided that at least one of R1, R2, R3, R4 or R5 is not hydrogen.

公开(公告)号：US20210060527A1

公开(公告)日：2021-03-04

申请号：US16959373

申请日：2019-01-07

Applicant: Puridify Ltd.

Inventor： Marc Hummersone ,  Benjamin Wallis ,  Frederic Leroux ,  William Howard Albert Law

IPC: B01J20/26 ,  B01J20/32 ,  B01J39/05 ,  B01J39/07 ,  B01J41/05 ,  B01J41/07 ,  B01J20/285 ,  B01D15/32 ,  B01D15/36

Abstract: The invention discloses a functionalised chromatography medium, comprising: i) at least one non-woven layer (10) of polymeric nano fibres (20) comprising a plurality of nanofibre-nano fibre fusion points (30); ii) a grafted polymer coating covering the polymeric nanofibres and the nanofibre-nanofibre fusion points; iii) a plurality of ligand groups covalently bound to the grafted polymer coating, wherein the ligand groups are capable of interacting with a target biomolecule.