NON-LIVING SURROGATE INDICATORS AND METHODS FOR SANITATION VALIDATION

    公开(公告)号:US20220018836A1

    公开(公告)日:2022-01-20

    申请号:US17344202

    申请日:2021-06-10

    Abstract: Systems, surrogates, indicators and methods for rapid assessment of sanitation processes are provided. Non-living and non-toxic surrogates applied to a platform or encapsulated in a biological material mounted to a platform are exposed to a sanitation process to be evaluated. Responses to sanitation are measured and quantified using FTIR and chemometrics including principal component analysis (PCA), partial least squares regression (PLSR), loading plots and predictive models. An artificial leaf platform with one or more types of surrogates on one surface and an anchor such as an adhesive film on a second surface is described. Surrogate types include nucleic acid, phage, yeast and algae surrogates. Surrogates may also be attached directly or through a polymer to the platform surface. Surrogates may also be encapsulated or attached to the outside of a biological carrier such as a yeast cell that is free or coupled to the platform.

    ANTIMICROBIAL PARTICLES AND SANITIZATION METHODS

    公开(公告)号:US20200337304A1

    公开(公告)日:2020-10-29

    申请号:US16871831

    申请日:2020-05-11

    Abstract: Rechargeable antimicrobial materials that can provide continuous sanitation of contact surfaces or liquids are provided that are based on functionalized sanitization particles that are either freestanding or coupled to a surface film. The sanitization particles have a carrier particle and one or more antimicrobial agents loaded into, adsorbed onto or bonded to each carrier particle. The antimicrobial agents may also be joined to a polymer or particulates of clay or zeolite that are coupled to the carrier. Sanitization particles may also be coupled directly to a film or through a chemical linker. The particles and films provide methods of enhanced inactivation of antibiotic resistant bacteria, inactivation and dissipation of biofilms, and the reduction or elimination of bacterial pathogens from surfaces, medical devices, on food or in food containers.

    Methods of selecting and isolating cancer stem cells

    公开(公告)号:US10509036B2

    公开(公告)日:2019-12-17

    申请号:US15586594

    申请日:2017-05-04

    Abstract: Provided herein are methods for selecting/identifying and/or isolating cancer stem cells from a biological sample or a cell culture sample using a fluorescent glucose analog. Also provided herein are methods for selecting/identifying and/or isolating leukemia stem cells and subpopulations thereof. The present invention is based, in part, on the discovery that cancer stem cells can be selected/identified based upon a lower level of fluorescence of the fluorescent glucose analog compared to non-cancer stem cells and that specific genes are differentially expressed in leukemia stem cells compared to non-leukemia stem cells.

    METHODS OF SELECTING AND ISOLATING CANCER STEM CELLS

    公开(公告)号:US20180011100A1

    公开(公告)日:2018-01-11

    申请号:US15586594

    申请日:2017-05-04

    Abstract: Provided herein are methods for selecting/identifying and/or isolating cancer stem cells from a biological sample or a cell culture sample using a fluorescent glucose analog. Also provided herein are methods for selecting/identifying and/or isolating leukemia stem cells and subpopulations thereof. The present invention is based, in part, on the discovery that cancer stem cells can be selected/identified based upon a lower level of fluorescence of the fluorescent glucose analog compared to non-cancer stem cells and that specific genes are differentially expressed in leukemia stem cells compared to non-leukemia stem cells.

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