Targeted Rolling Circle Amplification
    4.
    发明申请
    Targeted Rolling Circle Amplification 有权
    目标滚动圆放大

    公开(公告)号:US20140295498A1

    公开(公告)日:2014-10-02

    申请号:US14208451

    申请日:2014-03-13

    CPC classification number: C12Q1/6853 C12Q2521/301 C12Q2525/131 C12Q2531/125

    Abstract: Methods for amplifying a desired target region of a nucleic acid through rolling circle amplification with a strand-displacing polymerase are provided. Concatameric hairpin products are resolved with endonuclease digestion, and the resulting amplified product hairpins or fragments can be circularized and employed as templates in a subsequent round of amplification. The methods are effective for targeted amplification of even highly repetitive sequences. Compositions, kits, and systems related to or useful in the methods are also described.

    Abstract translation: 提供了通过用链置换聚合酶通过滚环扩增来扩增核酸的期望靶区域的方法。 通过核酸内切酶消化分解共发色的发夹产物,并且所得到的扩增产物发夹或片段可以被循环,并在随后的一轮扩增中用作模板。 该方法对于甚至高度重复序列的靶向扩增是有效的。 还描述了与方法相关或有用的组合物,试剂盒和系统。

    Targeted rolling circle amplification
    6.
    发明授权
    Targeted rolling circle amplification 有权
    目标滚圈放大

    公开(公告)号:US09290800B2

    公开(公告)日:2016-03-22

    申请号:US14208451

    申请日:2014-03-13

    CPC classification number: C12Q1/6853 C12Q2521/301 C12Q2525/131 C12Q2531/125

    Abstract: Methods for amplifying a desired target region of a nucleic acid through rolling circle amplification with a strand-displacing polymerase are provided. Concatameric hairpin products are resolved with endonuclease digestion, and the resulting amplified product hairpins or fragments can be circularized and employed as templates in a subsequent round of amplification. The methods are effective for targeted amplification of even highly repetitive sequences. Compositions, kits, and systems related to or useful in the methods are also described.

    Abstract translation: 提供了通过用链置换聚合酶通过滚环扩增来扩增核酸的期望靶区域的方法。 通过核酸内切酶消化分解共发色的发夹产物,并且所得到的扩增产物发夹或片段可以被循环,并在随后的一轮扩增中用作模板。 该方法对于甚至高度重复序列的靶向扩增是有效的。 还描述了与方法相关或有用的组合物,试剂盒和系统。

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