公开(公告)号：US20230364585A1

公开(公告)日：2023-11-16

申请号：US18144514

申请日：2023-05-08

Applicant: Waters Technologies Corporation

Inventor： Matthew A. Lauber ,  Catalin Doneanu ,  Weibin Chen ,  Ying Qing Yu ,  Jennifer M. Nguyen ,  Fabrice Gritti ,  Martin Gilar

IPC: B01J20/288 ,  B01J20/32 ,  B01J20/10 ,  B01J20/28

CPC classification number: B01J20/288 ,  B01J20/103 ,  B01J20/28004 ,  B01J20/3204 ,  B01J2220/54

Abstract: The present disclosure discusses a method of separating and/or purifying polynucleotides. The method includes injecting a sample into a chromatographic column that is packed with a porous sorbent having a pore size that substantially excludes the polynucleotides from the sorbent. This restricted access to the sorbent allows separation of large polynucleotides from each other and from smaller molecular weight impurities.

公开(公告)号：US11802879B2

公开(公告)日：2023-10-31

申请号：US16080903

申请日：2017-03-02

Applicant: Waters Technologies Corporation

Inventor： Liuxi Chen ,  Weibin Chen ,  Steven Cubbedge

IPC: G01N33/68 ,  G01N30/72 ,  H01J49/00 ,  G01N30/02

CPC classification number: G01N33/6848 ,  G01N30/72 ,  G01N30/7233 ,  G01N2030/027 ,  H01J49/004

Abstract: The present disclosure relates to a streamlined, complete workflow for the qualitative and the quantitative analysis of conjugated peptides from antibody-drug conjugate (ADC) compounds.

公开(公告)号：US20220268783A1

公开(公告)日：2022-08-25

申请号：US17314452

申请日：2021-05-07

Applicant: Waters Technologies Corporation

Inventor： Ximo Zhang ,  Stephan M. Koza ,  Ying Qing Yu ,  Weibin Chen

IPC: G01N33/68 ,  G01N30/20 ,  G01N30/22 ,  G01N30/72

Abstract: The present disclosure relates to a method of characterizing proteins in a sample. The method includes: removing non-ionic surfactant from the sample via denaturing size-exclusion chromatography to form a denatured sample; eluting the denatured sample via liquid chromatography to collect fractions of the sample, wherein the fractions of the sample include a protein fraction; lyophilizing the protein fraction to increase protein concentration; reconstituting the lyophilized protein fraction with a buffer comprising a surfactant to denature the protein; digesting the denatured protein fraction with an enzyme; and analyzing the digested protein fraction.

公开(公告)号：US10242853B2

公开(公告)日：2019-03-26

申请号：US15318254

申请日：2015-06-12

Applicant: Waters Technologies Corporation

Inventor： Scott J Geromanos ,  Steven J Ciavarini ,  Weibin Chen ,  Stephane Houel

IPC: H01J49/00 ,  G01N30/86 ,  G01N30/72

Abstract: A method of mass spectrometry comprises ionizing a sample eluting from a separation device in order to generate a plurality of parent ions. The method further comprises generating a target list of ions, which includes a predicted mass to charge ratio, a predicted chromatographic retention or elution time, and a predicted ion mobility drift time, derived from a model. Multiple cycles of operation are then performed as the sample elutes from the separation device. Each cycle of operation includes mass filtering the parent ions so that selected ions having mass to charge ratios within a first mass to charge ratio range are onwardly transmitted to a fragmentation or reaction device. The target list is then checked and the model is updated accordingly. The first mass to charge ratio range can then be adjusted in response to the updated model.