公开(公告)号：US20030013103A1

公开(公告)日：2003-01-16

申请号：US10126139

申请日：2002-04-19

Inventor： Bruce J. Bryan ,  Stephen Gaalema ,  Randall B. Murphy

IPC: C12Q001/68 ,  G01N033/53 ,  G01N033/542 ,  C12M001/34 ,  G01J003/42 ,  G01J003/427

CPC classification number: G01N33/582 ,  G01N21/253 ,  G01N21/763 ,  G01N33/54366 ,  Y10S435/808 ,  Y10S435/973 ,  Y10S435/975 ,  Y10S436/805 ,  Y10T436/186

Abstract: Solid phase methods for the identification of an analyte in a biological medium, such as a body fluid, using bioluminescence are provided. A chip designed for performing the method and detecting the bioluminescence is also provided. Methods employing biomineralization for depositing silicon on a matrix support are also provided. A synthetic synapse is also provided.

公开(公告)号：US20020127706A1

公开(公告)日：2002-09-12

申请号：US10053585

申请日：2002-01-24

Applicant: FUJI PHOTO FILM CO., LTD.

Inventor： Masayuki Naya ,  Takashi Kubo ,  Takashi Ito ,  Yoshimitsu Nomura

IPC: C12M001/34 ,  G01J003/42 ,  G01J003/427

CPC classification number: G01N21/553 ,  B29C45/0025 ,  B29C2045/0027 ,  G01N2201/0415

Abstract: A surface plasmon resonance measuring apparatus is provided with a dielectric block, a metal film formed on a surface of the dielectric block, a light source for emitting a light beam, an optical system for making the light beam enter the dielectric block at various angles of incidence so that a condition for total internal reflection is satisfied at an interface between the dielectric block and the thin film layer, and a photodetector for detecting the intensity of the light beam satisfying total internal reflection at the interface. In the measurement chip to be utilized in the surface plasmon resonance measuring apparatus, the dielectric block is formed from a synthetic resin in which, when said light beam is p-polarized outside said dielectric block and then strikes the interface, the intensity of a s-polarized component at the interface is 50% or less of the intensity of the light beam at the interface.

Abstract translation: 表面等离子体共振测定装置具备介质块，形成在介质块的表面上的金属膜，用于发射光束的光源，使光束以各种角度进入介质块的光学系统 入射，使得在介电块和薄膜层之间的界面处满足全内反射条件，以及用于检测在界面处满足全内反射的光束的强度的光电检测器。 在表面等离子体共振测定装置中使用的测量芯片中，介电块由合成树脂形成，其中当所述光束在所述介质块外部p偏振，然后撞击界面时，s的强度 界面处的极化分量为界面光束强度的50％以下。

公开(公告)号：US20020009744A1

公开(公告)日：2002-01-24

申请号：US09861137

申请日：2001-05-18

Inventor： Valery Bogdanov

IPC: C12Q001/68 ,  G01J003/427

CPC classification number: G01N21/6452 ,  G01N21/6428 ,  G01N21/6456 ,  G01N2021/6423

Abstract: The present invention provides a hyperspectral imaging apparatus and methods for employing such an apparatus for multi-dye/base detection of a nucleic acid molecule coupled to a solid surface.

公开(公告)号：US20020193948A1

公开(公告)日：2002-12-19

申请号：US10172563

申请日：2002-06-13

Applicant: CARL ZEISS JENA GmbH

Inventor： Dietrich Schweitzer ,  Lutz Leistritz ,  Martin Hammer ,  Karl-Heinz Donnerhacke

IPC: G06F019/00 ,  G01N033/48 ,  G01N033/50 ,  G06K009/00 ,  G01J003/42 ,  G01J003/427

CPC classification number: A61B3/12

Abstract: An arrangement and method for determining the two-dimensional distribution of fundus pigments, particularly of the xanthophyll macular pigment. The arrangement for carrying out the method comprises an illumination unit which illuminates the retina via an illumination beam path directed to the ocular fundus, observation optics located in the observation beam path proceeding from the ocular fundus, an image processing unit, elements for beam deflection and a central controlling and evaluating unit. In the method, a two-dimensional reflection image of the retina is recorded in a selected narrow-band wavelength region. In evaluating this two-dimensional reflection image, site-specific areas are established for determining the optical density and comparison values. The optical density of the fundus pigment at every fundus location is calculated from the negative logarithmic value of the quotient of the intensity value of the reflection image IR(null) at this fundus site to a comparison intensity value of the reflection image IR(null)Comparison. The suggested solution for the objective detection of the two-dimensional distribution of the optical density of the macular pigment xanthophyll is also suitable in principle for determining the distribution of other fundus pigments.

Abstract translation: 用于确定眼底颜料，特别是黄叶黄斑色素的二维分布的布置和方法。 用于执行该方法的装置包括：照明单元，其经由指向眼底的照明光束照射视网膜，位于观察光束路径中的观察光学器件，从眼底进入，图像处理单元，用于光束偏转的元件， 中央控制评估单位。 在该方法中，将视网膜的二维反射图像记录在所选择的窄带波长区域中。 在评估这种二维反射图像时，建立位置特定区域以确定光密度和比较值。 每个眼底位置的眼底颜料的光密度由该眼底位置的反射图像IR（lambd）的强度值的商数的负对数值与反射图像IR（lambd）的比较强度值计算， 比较。 用于目标检测黄斑色素叶黄素光密度二维分布的解决方案在原理上也适用于确定其他眼底颜料的分布。

公开(公告)号：US20020102595A1

公开(公告)日：2002-08-01

申请号：US10059754

申请日：2002-01-29

Inventor： Lloyd Mervyn Davis

IPC: C12Q001/68 ,  C12M001/34 ,  G01J003/42 ,  G01J003/427

CPC classification number: G01N15/14 ,  C12Q1/6869 ,  G01N2015/1415 ,  G01N2015/1438 ,  C12Q2533/101

Abstract: Methods and devices for detecting the incorporation of NTPs into immobilized enzyme-nucleic acid complexes are disclosed. The methods and devices can be used to genotype or sequence nucleic acids, including DNA and RNA, and are capable, in preferred embodiments, of detecting single incorporation events.

Abstract translation: 公开了用于检测NTPs结合到固定化酶 - 核酸复合物中的方法和装置。 方法和装置可用于对核酸（包括DNA和RNA）进行基因型或序列测定，并且在优选实施方案中能够检测单一掺入事件。

公开(公告)号：US20040038386A1

公开(公告)日：2004-02-26

申请号：US10363577

申请日：2003-03-04

Inventor： Wolfgang Zesch ,  Martin Andreas Bopp ,  Michael Pawlak

IPC: C12M001/34 ,  G01J003/42 ,  G01J003/427

CPC classification number: G01N21/6452 ,  G01N21/648 ,  G01N21/7703 ,  G01N2021/6419

Abstract: The invention relates to embodiments of an optical system for luminescence determination, comprising two or more excitation light sources, a sensor platform and an optical component with several discrete facets for beam deflection towards the sensor platform. Further subjects of the invention are methods for luminescence determination with an optical system according to the invention and analytical systems, as well as the use of these methods for quantitative affinity sensing and for various other applications. The aim of the present invention is to provide optical and analytical measuring devices for highly sensitive detection of one or more analytes, using a multitude of measurement areas on a common carrier.

Abstract translation: 本发明涉及用于发光确定的光学系统的实施例，其包括两个或更多个激发光源，传感器平台和具有用于朝向传感器平台的光束偏转的多个离散面的光学部件。 本发明的其它主题是使用根据本发明的光学系统和分析系统进行发光测定的方法，以及这些方法用于定量亲和力感测和各种其它应用的方法。 本发明的目的是提供使用在公共载体上的多个测量区域对一种或多种分析物进行高灵敏度检测的光学和分析测量装置。

公开(公告)号：US20030087282A1

公开(公告)日：2003-05-08

申请号：US10229204

申请日：2002-08-26

Applicant: Hitachi, Ltd. Incorporation: Japan

Inventor： Yoshitada Oshida ,  Satoshi Takahashi ,  Kenji Yasuda ,  Taisaku Seino

IPC: C12Q001/68 ,  G06K009/00 ,  G01J003/42 ,  G01J003/427 ,  G01N033/543

CPC classification number: G01N21/6428 ,  G01J3/4406 ,  G01N21/6452

Abstract: A wide-dynamic range, high-resistivity and high-speed method of stably obtaining fluorescent images of two or more samples which may includes a fluorescent material, comprising the steps of: disposing each of said samples onto each of two or more compartments which an area on a substrate is split into; sequentially irradiating said compartments with an exciting light wherein each of said compartments is irradiated with said exciting light with the intensity changed; detecting one or more fluorescent lights as generated from each of said samples corresponding to said changed intensity of said exciting light every each of said samples; determining the true value of fluorescence as generated from each of said samples by using information on said one or more fluorescent lights as detected every each of said samples; and obtaining a fluorescent image of each of said samples by using said true value of fluorescence from each of said samples, and an apparatus for carrying out the same are provided.

Abstract translation: 一种宽动态范围，高电阻率和高速方法，可稳定地获得可包括荧光材料的两个或多个样品的荧光图像，其包括以下步骤：将每个所述样品置于两个或更多隔室中的每一个上， 将基板上的区域分割成; 用激发光顺序地照射所述隔室，其中每个所述隔间用强度改变的所述激发光照射; 从每个所述样本中检测出一个或多个荧光灯，其对应于所述每个所述样本的所述激发光的所述改变的强度; 通过使用每个所述样品检测到的所述一个或多个荧光灯的信息来确定从每个所述样品产生的荧光的真实值; 并且通过使用来自每个所述样品的所述真实值的荧光获得每个所述样品的荧光图像，并且提供了用于进行所述样品的装置。

公开(公告)号：US20030059855A1

公开(公告)日：2003-03-27

申请号：US10180374

申请日：2002-06-26

Applicant: SRU Biosystems, LLC

Inventor： Brian T. Cunningham ,  Peter Y. Li

IPC: C12Q001/68 ,  G01N033/53 ,  G01N033/542 ,  C12M001/34 ,  G01J003/42 ,  G01J003/427

CPC classification number: G01N21/253 ,  B01L3/5085 ,  C12Q1/68 ,  G01J3/42 ,  G01N21/31 ,  G01N21/4788 ,  G01N21/55 ,  G01N21/75 ,  G01N21/7743 ,  G01N33/54373 ,  G01N2021/7773 ,  G01N2021/7776 ,  G02B5/1809

Abstract: Method and apparatus for detecting biomolecular interactions. The use of labels is not required and the methods may be performed in a high-throughput manner. An instrument system for detecting a biochemical interaction on a biosensor. The system includes an array of detection locations comprises a light source for generating collimated white light. A beam splitter directs the collimated white light towards a surface of a sensor corresponding to the detector locations. A detection system includes an imaging spectrometer receiving the reflected light and generating an image of the reflected light.

Abstract translation: 用于检测生物分子相互作用的方法和装置。 不需要使用标签，并且可以以高通量的方式执行方法。 用于检测生物传感器上的生物化学相互作用的仪器系统。 该系统包括一组检测位置，包括用于产生准直白光的光源。 分束器将准直的白光引导到对应于检测器位置的传感器的表面。 检测系统包括接收反射光并产生反射光的图像的成像光谱仪。

公开(公告)号：US20040115796A1

公开(公告)日：2004-06-17

申请号：US10721216

申请日：2003-11-26

Inventor： Ralph E. Burns

IPC: C12M001/34 ,  G01J003/42 ,  G01J003/427

CPC classification number: C12Q1/6834 ,  B01F9/0001 ,  B01F9/0014 ,  B01F9/0021 ,  B01F2009/0074 ,  B01L7/52 ,  B01L7/5255 ,  B01L9/06 ,  B01L2200/147 ,  B01L2300/1822 ,  B01L2300/1827 ,  B03C1/282 ,  B03C1/288 ,  B03C1/30 ,  B03C2201/26 ,  C12Q1/6813 ,  C12Q1/6832 ,  C12Q2537/10 ,  G01N35/0098 ,  G01N35/0099 ,  G01N35/025 ,  G01N35/1065 ,  G01N2035/00356 ,  G01N2035/00524 ,  G01N2035/0437 ,  G01N2035/0455 ,  G01N2035/103 ,  Y10T29/53657 ,  Y10T29/53683 ,  Y10T29/53843 ,  Y10T156/1105 ,  Y10T156/1906 ,  Y10T436/11 ,  Y10T436/111666 ,  Y10T436/113332 ,  Y10T436/114998 ,  Y10T436/119163 ,  Y10T436/2575

Abstract: An automated analyzer for performing multiple diagnostic assays simultaneously includes multiple stations, or modules, in which discrete aspects of the assay are performed on fluid samples contained in reaction receptacles. The analyzer includes stations for automatically preparing a specimen sample, incubating the sample at prescribed temperatures for prescribed periods, preforming an analyte isolation procedure, and ascertaining the presence of a target analyte. An automated receptacle transporting system moves the reaction receptacles from one station to the next. The analyzer further includes devices for carrying a plurality of specimen tubes and disposable pipette tips in a machine-accessible manner, a device for agitating containers of target capture reagents comprising suspensions of solid support material and for presenting the containers for machine access thereto, and a device for holding containers of reagents in a temperature controlled environment and presenting the containers for machine access thereto. A method for performing an automated diagnostic assay includes an automated process for isolating and amplifying a target analyte. The process is performed by automatically moving each of a plurality of reaction receptacles containing a solid support material and a fluid sample between stations for incubating the contents of the reaction receptacle and for separating the target analyte bound to the solid support from the fluid sample. An amplification reagent is added to the separated analyte after the analyte separation step and before a final incubation step.

Abstract translation: 用于执行多个诊断测定的自动分析仪同时包括多个站或模块，其中对反应容器中包含的流体样品进行分析方面的分析。 分析仪包括用于自动准备标本样品，在规定温度下规定时间孵育样品，预处理分析物分离程序以及确定目标分析物的存在的站。 自动插座输送系统将反应容器从一个站移动到另一个站。 分析仪还包括用于以机器可接近的方式携带多个样本管和一次性移液管尖端的装置，用于搅拌包含固体支持材料的悬浮液并用于提供用于机器通入的容器的靶捕获试剂的容器的装置，以及 用于在温度受控的环境中容纳试剂容器的装置，并且提供用于机器通入的容器。 执行自动诊断测定的方法包括用于分离和扩增靶分析物的自动化方法。 该过程通过在站之间自动移动包含固体支持材料和流体样品的多个反应容器中的每一个来进行，用于孵育反应容器的内容物并将与固体支持物结合的目标分析物与流体样品分离。 在分析物分离步骤之后和最终孵育步骤之前，将分离的分析物加入扩增试剂。

公开(公告)号：US20040061854A1

公开(公告)日：2004-04-01

申请号：US10451868

申请日：2003-06-25

Inventor： Dwight Sherod Walker ,  Mark Austin Patrick

IPC: G01J003/427

CPC classification number: G01N21/05 ,  G01N21/31

Abstract: A system and method for in vitro analysis of therapeutic agents comprising a reservoir adapted to hold a therapeutic agent, a first flow cell having a first cell chamber adapted to receive at least a first sample of the therapeutic agent, a second flow cell having a second cell chamber adapted to receive at least a second sample of the therapeutic agent, the first flow cell having a first path length (benull) and the second flow cell having a second path length (benull), the first path length being substantially equal to a sensitivity factor (f)nullbenull, a membrane chamber having a biological cell membrane therein adapted to receive at least a third sample of the therapeutic agent, the membrane chamber being further adapted to detect the membrane potential of the biological cell membrane; and spectroscopic detection means for detecting the spectral characteristics of the first and second therapeutic agent samples.

Abstract translation: 一种用于体外分析治疗剂的系统和方法，所述治疗剂包括适于保持治疗剂的贮存器，具有适于接收所述治疗剂的至少第一样品的第一细胞室的第一流动池，具有第二流体池 细胞室，其适于接收治疗剂的至少第二样品，所述第一流动池具有第一路径长度（be'），所述第二流动池具有第二路径长度（be“），所述第一路径长度基本上 等于灵敏度因子（f）xbe“，其中具有生物细胞膜的膜室适于接纳治疗剂的至少第三样品，膜室还适于检测生物细胞膜的膜电位 ; 以及用于检测第一和第二治疗剂样品的光谱特性的光谱检测装置。