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公开(公告)号：KR101141733B1
公开(公告)日：2012-05-03
申请号：KR1020030088987
申请日：2003-12-09
Applicant: 재단법인서울대학교산학협력재단
IPC: C12N5/16
Abstract: 본 발명은 유전자 적중 및 도입 기술과 체세포 복제 기술을 접목한 특정 질병에 대하여 내성을 가진 형질전환 복제동물의 생산방법 및 이 방법에 의해 생산된 복제동물에 관한 것이다.
구체적으로, 본 발명의 광우병에 내성을 가지는 형질전환 복제 소의 생산방법은 소 유래의 체세포의 프리온을 코딩하는 유전자를 적중시켜 형질전환 체세포를 제작하고, 이를 소 유래의 탈핵 난자에 도입하여 프리온 유전자가 적중된 핵 이식란을 작제하여, 이를 대리모에 이식하여 광우병 내성 소를 생산하는 단계를 포함한다.
또한, 본 발명의 형질전환 핵 이식란 및 복제 소는 정상적인 프리온이 적중되어 광우병에 내성을 가지는 것을 특징으로 한다.
형질전환 복제소, 체세포 복제, 광우병, 프리온 유전자 적중, 광우병 내성-
公开(公告)号：KR1020090123340A
公开(公告)日：2009-12-02
申请号：KR1020080049348
申请日：2008-05-27
Applicant: 재단법인서울대학교산학협력재단
IPC: A61B17/425 , A61D19/04
Abstract: PURPOSE: A method for transplanting embryo of a mammal is provided to improve conception efficiency by minimizing an external exposure time of an egg fertilized in vitro and a duplication fertilized egg in a process transplanting embryo of a mammal. CONSTITUTION: An immature ovum is collected from a mammal except for a human, and is cultivated in an incubator for 1~44 hours. A matured ovum is externally fertilized for six hours. A fertilized egg is absorbed by an embryo transplanting device of a straw shape, and is a fertilized egg of a first cell stage to a fourth cell stage. The embryo transplanting device is inserted inside oviduct of a surrogate mother of the mammal. The fertilized egg inside the embryo transplanting device is transplanted to the oviduct of the surrogate mother of the mammal.
Abstract translation: 目的：提供一种移植哺乳动物胚胎的方法，以通过在哺乳动物移植胚胎的过程中最小化体外受精卵和复制受精卵的外部暴露时间来提高受精效率。 构成：从哺乳动物除了人类之外收集未成熟的卵子，并在培养箱中培养1〜44小时。 成熟的卵子外部受精6小时。 受精卵被秸秆形状的胚胎移植装置吸收，是第一细胞阶段到第四细胞期的受精卵。 将胚胎移植装置插入到哺乳动物的替代母亲的输卵管内。 胚胎移植装置内的受精卵被移植到哺乳动物的替代母亲的输卵管中。
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公开(公告)号：KR1020080088947A
公开(公告)日：2008-10-06
申请号：KR1020070031865
申请日：2007-03-30
Applicant: 재단법인서울대학교산학협력재단 , 주식회사 모베이스
Abstract: A cell isolated from internal organ-surface corpuscle and duct is provided to identify cytological function of corpuscle and duct, collect multifunctional cells without surgical operation or invasive method, and differentiate the collected multifunctional cells into various cells of human body. A cell is isolated from corpuscle and duct on the surface of an internal organ selected from kidney, large intestine, small intestine and bladder. A visualizing method of the cell isolated from internal organ-surface corpuscle and duct comprises the steps of: verifying at least one position of corpuscle and duct on the surface of at least one internal organ selected from kidney, large intestine, small intestine and bladder; adding alcian blue into at least one from internal organ-surface corpuscle and duct and observing it with a microscope; collecting at least one from the alcian blue-colored internal organ-surface corpuscle and duct; double-immunostaining the collected internal organ-surface corpuscle or duct with a primary antibody; staining the double-immunostained internal organ-surface corpuscle or duct with a secondary antibody; and observing the secondary antibody-stained internal organ-surface corpuscle or duct with a microscopy and obtaining an image. Further, the organ-surface corpuscle has Hematologic system cell, Mesenchymal cell and extracellular matrix.
Abstract translation: 提供从内部器官表面微粒和管道分离的细胞，以鉴别细胞和管道的细胞学功能，收集多功能细胞，无需外科手术或侵入性方法，并将收集的多功能细胞分化为人体各种细胞。 细胞从选自肾，大肠，小肠和膀胱的内脏器官表面的小体和管道分离。 从内部器官表面微粒和管道分离的细胞的可视化方法包括以下步骤：验证选自肾，大肠，小肠和膀胱中的至少一种内脏的表面上的小体和管道的至少一个位置; 将Alcian蓝色至少从内部器官表面微粒和管道中加入，并用显微镜观察; 从阿尔派蓝色内部器官表面微粒和管道收集至少一个; 用第一抗体对收集的内部器官表面微粒或管道进行双重免疫染色; 用二级抗体染色双免疫染色的内部器官表面微粒或管道; 并用显微镜观察二次抗体染色的内部器官表面微粒或管道并获得图像。 此外，器官表面小体具有血液系统细胞，间充质细胞和细胞外基质。
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公开(公告)号：KR100824218B1
公开(公告)日：2008-04-25
申请号：KR1020060128318
申请日：2006-12-15
Applicant: 재단법인서울대학교산학협력재단
Abstract: A method for producing a cloned wolf is provided to contribute to preserve a rare and endangered species wolf and develop veterinary medicine, anthropology and medicine study fields. A method for preparing a nuclear transplant embryo of wolf comprises the steps of: (a) removing a nucleus from ovum of a canine animal such as dog, wolf, fox, jackal, coyote, dhole, and raccoon dog to prepare a denucleated ovum; (b) isolating a somatic cell from a tissue of the wolf to prepare a donor nucleus cell; (c) micro-injecting the donor nucleus cell into the denucleated ovum and electrofusing it; and (d) activating the fused ovum. A nuclear transplant embryo prepared by the method is deposited as a deposition no. KCTC 10903BP. A method for producing a cloned wolf comprises a step of transplanting the nuclear transplant embryo into an oviduct of a canine animal surrogate mother. Further, the electrofusing step is performed at a voltage of 3.6 to 4.1 kv/cm, for 10 to 30 mus, 1 to 3 times.
Abstract translation: 提供了克隆狼的生产方法，有助于保护珍稀濒危物种狼，开发兽药，人类学和医学研究领域。 制备狼的核移植胚胎的方法包括以下步骤：（a）从狗，狼，狐狸，豺狼，土拨鼠，hole和浣熊犬等动物的卵中除去核，以制备脱核卵; （b）从狼的组织中分离体细胞以制备供体核细胞; （c）将供体核细胞微注射到去核的卵中并将其电铸; 和（d）激活融合的卵子。 通过该方法制备的核移植胚胎被沉积为沉积物。 KCTC 10903BP。 克隆的狼的制备方法包括将核移植胚胎移植到犬动物替代母亲的输卵管中的步骤。 此外，电熔步骤在3.6至4.1kv / cm的电压下进行10至30次，1至3次。
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公开(公告)号：KR1020070090175A
公开(公告)日：2007-09-05
申请号：KR1020077012527
申请日：2005-05-23
Applicant: 재단법인서울대학교산학협력재단
IPC: G01N33/483 , G01N33/48 , B82Y15/00
Abstract: A visualizing agent comprising a magnetic nanoparticle is provided to observe the intravascular threadlike structure in the lymphatic vessel by dyeing it with the magnetic nanoparticle labeled with a luminescent organic dye through a microscope, so that it is useful for study on the cellular anatomy and physiology. A visualizing method of Bonghan duct in a lymphatic vessel comprises the steps of: injecting the magnetic nanoparticle labeled with a luminescent organic dye which dyes in situ or in vivo Bonghan duct into the lymphatic tissue; and observing it with a microscope, and further comprises supplying the magnetic field of 0.1-0.5 Tesla to the lymphatic tissue for 1-120 minutes, wherein the microscope is stereomicroscope, light microscope, inverted microscope, confocal laser scanning microscope or cryo scanning electron microscope; the magnetic nanoparticle contains a cobalt-ferrite particle core and an amorphous silica shell coating the core; the surface of magnetic nanoparticle is modified by a biocompatible polymer; and the organic dye is rhodamine B isothiocyanate or fluorescein isothiocyanate.
Abstract translation: 提供包含磁性纳米颗粒的可视化剂，以通过用显微镜将荧光有机染料标记的磁性纳米颗粒进行染色来观察淋巴管中的血管内线状结构，从而可用于细胞解剖和生理学的研究。 Bonghan导管在淋巴管中的可视化方法包括以下步骤：将在原位或体内Bonghan导管染色的发光有机染料标记的磁性纳米颗粒注射到淋巴组织中; 并用显微镜观察，进一步包括向淋巴组织提供0.1-0.5特斯拉的磁场1-120分钟，其中显微镜是立体显微镜，光学显微镜，倒置显微镜，共焦激光扫描显微镜或低温扫描电子显微镜 ; 磁性纳米粒子含有钴铁氧体颗粒芯和涂覆芯的无定形二氧化硅壳; 磁性纳米颗粒的表面被生物相容性聚合物改性; 有机染料是异硫氰酸罗丹明B或异硫氰酸荧光素。
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Patent Agency Ranking
公开(公告)号：KR1020050022416A
公开(公告)日：2005-03-08
申请号：KR1020030060612
申请日：2003-08-30
Applicant: 재단법인서울대학교산학협력재단
IPC: C12N5/16
CPC classification number: C12N15/8778 , A01K67/0273 , A01K2227/108 , A01K2267/01 , A61D19/04 , C12N15/8509 , C12N2015/8518 , C12N2517/00
Abstract: PURPOSE: A transgenic cloned pig producing human erythropoietin and a method for producing the same transgenic cloned pig are provided, thereby producing a large quantity of human erythropoietin in the urinary bladder of the transgenic cloned pig with reduced costs. CONSTITUTION: The nuclear transfer embryo produced by a fusion of a pig-derived enucleated oocyte with a nucleus of a pig-derived somatic cell transfected and targeted with a gene encoding human erythropoietin is provided. The transgenic cloned pig expresses human erythropoietin in the urinary bladder of pig. The method for producing a transgenic cloned pig producing human erythropoietin comprises the steps of: (a) transfecting and targeting a gene encoding human erythropoietin into a pig-derived somatic cell line to prepare a nuclear donor cell; (b) removing cumulus cells surrounding a recipient oocyte and then the cytoplasm including the first polar body from the oocyte to prepare a pig-derived enucleated recipient oocyte; (c) transferring the transfected nuclear donor cell into the enucleated recipient oocyte and carrying out cell fusion to generate nuclear transfer embryos; and (d) transplanting the nuclear transfer embryo into a surrogate mother pig to produce live offspring.
Abstract translation: 目的：提供产生人促红细胞生成素的转基因克隆猪和产生相同转基因克隆猪的方法，从而以降低的成本在转基因克隆猪的膀胱中产生大量人促红细胞生成素。 构成：提供了通过将猪衍生的去核卵母细胞与转染并用编码人红细胞生成素的基因靶向的来源于体细胞的细胞核的融合产生的核转移胚。 转基因克隆猪在猪的膀胱中表达人促红细胞生成素。 生产人促红细胞生成素的转基因克隆猪的方法包括以下步骤：（a）将编码人促红细胞生成素的基因转染并靶向猪衍生的体细胞系以制备核供体细胞; （b）去除卵母细胞周围的卵丘细胞，然后从卵母细胞中除去包括第一极体的细胞质，以制备猪衍生的去核受体卵母细胞; （c）将转染的核供体细胞转移到去核的受体卵母细胞中并进行细胞融合以产生核转移胚胎; 和（d）将核转移胚胎移植到替代母猪中以产生活的后代。
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公开(公告)号：KR1020040016426A
公开(公告)日：2004-02-21
申请号：KR1020030056629
申请日：2003-08-14
Applicant: 재단법인서울대학교산학협력재단
IPC: C12N5/16
CPC classification number: A01K67/0273 , A01K67/0275 , A01K2227/101 , A01K2267/01 , C12N9/6456 , C12N15/8509 , C12N15/8771 , C12N2830/008
Abstract: PURPOSE: A transgenic cloned cow producing human prourokinase and a method for producing the same are provided, thereby expressing human prourokinase from the mammary gland of the transgenic cloned cow, and easily separating it from milk. CONSTITUTION: Provided is a nucleus transplanted ovum wherein a nucleus of a somatic cell derived from a cow and containing a human prourokinase gene is fused with a nucleus removed ovum derived from cow. The nucleus transplanted ovum is SNU-B1(KCTC 10286BP). A method for producing the nucleus transplanted ovum comprises the steps of: (a) introducing a human prourokinase gene into the somatic cell derived from cow to prepare a nucleus transferring cell; (b) removing a cumulus cell and cytoplasm from a ovum of a cow to prepare a nucleus receiving ovum; and (c) transplanting the nucleus transferring cell into the nucleus receiving ovum and fusing them.
Abstract translation: 目的：提供产生人类原尿激酶的转基因克隆牛及其生产方法，从而从转基因克隆牛的乳腺中表达人类原尿激酶，并且容易地将它与牛分离。 构成：提供了一种移植卵的细胞核，其中源自牛的体细胞细胞核并含有人类原尿激酶基因与从牛获得的去除卵的细胞核融合。 移植的卵细胞为SNU-B1（KCTC 10286BP）。 用于产生移植卵的细胞的方法包括以下步骤：（a）将人类原尿激酶基因导入来自牛的体细胞，制备细胞核转移细胞; （b）从牛的卵中除去卵丘细胞和细胞质以制备接受卵的细胞核; 和（c）将细胞核转移细胞移植到接受卵子的细胞核中并融合它们。
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公开(公告)号：KR101046706B1
公开(公告)日：2011-07-05
申请号：KR1020080049348
申请日：2008-05-27
Applicant: 재단법인서울대학교산학협력재단
IPC: A61B17/425 , A61D19/04
Abstract: 본 발명은 스트로우(Straw) 형 수정란 이식기구를 이용하여 포유동물의 수정란을 포유동물 대리모의 난관 내로 이식하는 방법에 관한 것이다. 보다 구체적으로 본 발명은 수정란 이식을 위하여 수정란을 난관 내로 주입하는 과정에서 스트로우를 이용함으로써 기존의 이식 카테터를 통한 이식 방법과 비교하여, 그 시술법을 간단히 하고, 외부 대기 노출 시간을 줄임으로써 수태 효율을 개선하여 복제 동물 및 체외수정 동물의 생산 효율을 향상시키기 위한 방법에 관한 것이다.
본 발명에 따른 수정란 이식방법은 기존의 방법과 비교하여 그 시술방법이 간단하며, 수정란의 대기 노출 시간을 단축시킬 수 있어 수정란의 손상을 방지할 수 있으며, 수정란 이식 효율을 개선시킬 수 있으므로 우수품종 개량, 이종 장기용 돼지 생산, 질환 모델동물 생산 등의 축산, 수의학, 의학연구 분야의 발달에 기여할 수 있다.
돼지, 수정란 이식, 난관, 스트로우-
公开(公告)号：KR1020070068557A
公开(公告)日：2007-07-02
申请号：KR1020050130272
申请日：2005-12-27
Applicant: 재단법인서울대학교산학협력재단 , 주식회사 모베이스
IPC: G01N31/22
Abstract: Provided is a visualizing agent comprising alcian blue, which is administered into blood and lymph vessels so as to stain a thread-like structure present therein in a free state and observe the structure via a microscope. The visualizing agent comprises a dye for visualizing a so-called Bonghan duct present in blood and lymph vessels. The visualizing agent performs in situ or in vivo visualization of the Bonghan duct by staining it to a higher degree than the blood and lymph vessels. The visualizing agent comprises alcian blue. Particularly, the visualizing agent comprises 1g of 60% alcian blue and 2,000 units of heparin per 100 ml of phosphate buffered saline.
Abstract translation: 本发明提供一种含有阿片蓝的可视化剂，将其投入血液和淋巴管中，使自由状态下的丝状结构染色，并通过显微镜观察结构。 可视化剂包括用于使存在于血液和淋巴管中的所谓Bonghan导管可视化的染料。 可视化剂通过将其染色至比血液和淋巴管更高的程度，进行Bonghan导管的原位或体内可视化。 可视化剂包括阿尔卑斯蓝。 特别地，可视化剂包含1g每100ml磷酸盐缓冲盐水中的60％阿尔卑斯蓝和2,000单位肝素。
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公开(公告)号：KR100733012B1
公开(公告)日：2007-06-28
申请号：KR1020050067736
申请日：2005-07-26
Applicant: 재단법인서울대학교산학협력재단
IPC: C12N5/16
CPC classification number: C12N15/877
Abstract: 본 발명은 복제된 개과 동물 및 이의 생산방법에 관한 것이다. 보다 구체적으로 본 발명은 개과 동물의 성숙 난자로부터 핵을 제거하여 탈핵 난자를 제조한 다음 개과 동물의 체세포를 공여 핵 세포로 이용하여 상기 탈핵 난자에 최적화된 조건으로 핵 이식을 수행하여 핵 이식란을 제조하고, 이를 대리모의 난관에 이식하는 것을 특징으로 하는 복제된 개과 동물의 생산방법 및 상기 방법에 의해 생산된 복제된 개과 동물에 관한 것이다.
본 발명에 따른 방법은 복제된 개과 동물을 생산하는 효과가 있으며 이는 우량 개과 동물의 번식, 희귀· 멸종 위기의 개과 동물의 보존, 이종이식, 질환 모델동물 등 수의학, 인류학 및 의학연구 분야의 발달에 기여할 수 있다.
복제, 개과 동물, 핵 이식, 체내 성숙 난자
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