公开(公告)号：KR1020050023483A

公开(公告)日：2005-03-10

申请号：KR1020030059730

申请日：2003-08-28

Applicant: 재단법인서울대학교산학협력재단

Inventor： 황우석 ,  이병천 ,  강성근 ,  용환율 ,  현상환

IPC: C12M3/10

Abstract: PURPOSE: An improved method of intracytoplasmic sperm injection is provided, thereby reducing the medium amount used, inhibiting damage of oocyte and inducing mechanical damage of the sperm head membrane, so that the fertilization success rate can be improved. CONSTITUTION: An injection pipette having the diameter which is larger than the maximum head diameter of sperms to be introduced into the oocyte cytoplasm and is smaller than the maximum tail diameter of sperms is used in the intracytoplasmic sperm injection(ICSI), wherein the end of the pipette has a plurality of spikes. The improved method of intracytoplasmic sperm injection comprises the steps of: collecting a sperm with the injection pipette; introducing the sperm into the oocyte cytoplasm; and recovering the injection pipette, wherein the sperm head is collected by the tip of the injection pipette.

Abstract translation: 目的：提供细胞质内精子注射的改进方法，减少使用的培养基，抑制卵母细胞的损伤，诱导精子头膜的机械损伤，从而提高受精成功率。 构成：在细胞质内精子注射（ICSI）中使用直径大于要引入卵母细胞的精子的最大头部直径并且小于精子的最大尾部直径的注射移液管，其中末端 移液管具有多个尖峰。 细胞质内精子注射的改进方法包括以下步骤：用注射移液管收集精子; 将精子引入卵母细胞质中; 并回收注射移液管，其中精子头被注射移液管的尖端收集。

公开(公告)号：KR1020040074108A

公开(公告)日：2004-08-21

申请号：KR1020047010333

申请日：2001-12-29

Applicant: 재단법인서울대학교산학협력재단

Inventor： 황우석 ,  이병천 ,  강성근 ,  한재용 ,  임정묵 ,  이창규 ,  이은송 ,  정의배 ,  조종기 ,  김대영 ,  현상환 ,  이갑상 ,  김혜수 ,  이소현 ,  이성철 ,  염수청

IPC: A01K67/02 ,  C12N5/07 ,  C12N15/63

Abstract: PURPOSE: A GFP(Green Fluorescent Protein)-transfected clone pig, a GT(alpha 1, 3 galactrosyltransferase) knock-out clone pig and a production method thereof are provided to produce a clone pig with a determined genetic character using transfection and targeting technologies. CONSTITUTION: The production method of the GFP-transfected clone pig comprises the steps of: preparing a cell line taken and cultured from a pig as a nucleus donating cell; introducing the GFP clone to the nucleus donating cell and expressing by forming a lipid(or a positive charge polymer)-DNA structure complex, adding the complex to the nucleus donating cell culture solution and culturing; producing a transfected embryo by transplanting the cell to an enucleated pig embryo and activating; and producing a piglet by transplanting the embryo to a surrogate mother.

Abstract translation: 目的：提供GFP（绿色荧光蛋白）转染的克隆猪，GT（α1,3半乳糖基转移酶）敲除克隆猪及其制备方法，以使用转染和靶向技术产生具有确定遗传特征的克隆猪 。 构成：GFP转染的克隆猪的制备方法包括以下步骤：制备从猪获得并培养为细胞核的细胞系; 将GFP克隆引入提供细胞核并通过形成脂质（或正电荷聚合物）-DNA结构复合物进行表达，将复合物加入给予细胞培养液的细胞核并培养; 通过将细胞移植到去核的猪胚胎并激活来产生转染的胚胎; 并通过将胚胎移植到替代母亲来生产仔猪。

公开(公告)号：KR100505124B1

公开(公告)日：2005-07-29

申请号：KR1020030059730

申请日：2003-08-28

Applicant: 재단법인서울대학교산학협력재단

Inventor： 황우석 ,  이병천 ,  강성근 ,  용환율 ,  현상환

IPC: C12M3/10

Abstract: 본 발명은 통상적인 ICSI 방법이 가지는 문제점을 해소하고 수정율을 향상시키기 위해 개선된 ICSI 방법 및 이를 가능하게 하는 주입용 피펫에 관한 것이다.
보다 구체적으로, 본 발명은 정자 두부 막의 기계적인 손상을 유발하도록 형성된 주입용 피펫을 사용하여 정상적인 수정 과정에 더 근접한 환경을 제공할 수 있는 새로운 ICSI 방법에 관한 것이다.
본 발명의 위와 같은 효과는 주입용 피펫의 내경을 주입하고자 하는 정자의 두부의 최대 직경보다 크지 않게 구성하고 주입과 동시에 기계적인 손상을 유발함으로써 가능하다.

公开(公告)号：KR1020050022416A

公开(公告)日：2005-03-08

申请号：KR1020030060612

申请日：2003-08-30

Applicant: 재단법인서울대학교산학협력재단

Inventor： 황우석 ,  이병천 ,  강성근 ,  현상환 ,  이갑상 ,  김혜수 ,  김수 ,  정연우 ,  남동현 ,  전현용 ,  염수청 ,  김순웅

IPC: C12N5/16

CPC classification number: C12N15/8778 ,  A01K67/0273 ,  A01K2227/108 ,  A01K2267/01 ,  A61D19/04 ,  C12N15/8509 ,  C12N2015/8518 ,  C12N2517/00

Abstract: PURPOSE: A transgenic cloned pig producing human erythropoietin and a method for producing the same transgenic cloned pig are provided, thereby producing a large quantity of human erythropoietin in the urinary bladder of the transgenic cloned pig with reduced costs. CONSTITUTION: The nuclear transfer embryo produced by a fusion of a pig-derived enucleated oocyte with a nucleus of a pig-derived somatic cell transfected and targeted with a gene encoding human erythropoietin is provided. The transgenic cloned pig expresses human erythropoietin in the urinary bladder of pig. The method for producing a transgenic cloned pig producing human erythropoietin comprises the steps of: (a) transfecting and targeting a gene encoding human erythropoietin into a pig-derived somatic cell line to prepare a nuclear donor cell; (b) removing cumulus cells surrounding a recipient oocyte and then the cytoplasm including the first polar body from the oocyte to prepare a pig-derived enucleated recipient oocyte; (c) transferring the transfected nuclear donor cell into the enucleated recipient oocyte and carrying out cell fusion to generate nuclear transfer embryos; and (d) transplanting the nuclear transfer embryo into a surrogate mother pig to produce live offspring.

Abstract translation: 目的：提供产生人促红细胞生成素的转基因克隆猪和产生相同转基因克隆猪的方法，从而以降低的成本在转基因克隆猪的膀胱中产生大量人促红细胞生成素。 构成：提供了通过将猪衍生的去核卵母细胞与转染并用编码人红细胞生成素的基因靶向的来源于体细胞的细胞核的融合产生的核转移胚。 转基因克隆猪在猪的膀胱中表达人促红细胞生成素。 生产人促红细胞生成素的转基因克隆猪的方法包括以下步骤：（a）将编码人促红细胞生成素的基因转染并靶向猪衍生的体细胞系以制备核供体细胞; （b）去除卵母细胞周围的卵丘细胞，然后从卵母细胞中除去包括第一极体的细胞质，以制备猪衍生的去核受体卵母细胞; （c）将转染的核供体细胞转移到去核的受体卵母细胞中并进行细胞融合以产生核转移胚胎; 和（d）将核转移胚胎移植到替代母猪中以产生活的后代。