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公开(公告)号：KR1020120000405A
公开(公告)日：2012-01-02
申请号：KR1020100060756
申请日：2010-06-25
Applicant: 전남대학교산학협력단 , 김재민
Abstract: PURPOSE: A method for producing a large amount of cyanobacteria is provided to improve productivity and to enhance growth and immunity. CONSTITUTION: A method for producing a large amount of cyanobacteria comprises: a step of inoculating germs into a medium and culturing at 3500-5800lux and 30-35°C; a step of isolating cyanobacteria cultures in the medium; and a step of processing the isolated cyanobacteria by drying. The medium is an organic medium using pig stool powder. The isolation is performed using a filter.
Abstract translation: 目的：提供一种生产大量蓝细菌的方法，以提高生产率和增强生长和免疫力。 构成：生产大量蓝细菌的方法包括：将细菌接种到培养基中并在3500-5800lux和30-35℃培养的步骤; 在培养基中分离蓝细菌培养物的步骤; 以及通过干燥处理孤立的蓝细菌的步骤。 培养基是使用粪便粉末的有机培养基。 使用过滤器执行隔离。
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公开(公告)号：KR1020110011033A
公开(公告)日：2011-02-08
申请号：KR1020090068468
申请日：2009-07-27
Applicant: 전남대학교산학협력단 , 김재민
Abstract: PURPOSE: A nutrition enhancing agent for marine cultivation is provided to promote the growth of shellfish and fish eating marine microalgae and to enhance the immune function of the shellfish and fish. CONSTITUTION: A nutrition enhancing agent for marine cultivation is produced by the following steps: cultivating microalgae at a density of 10million cell/ml; condensing the cultivated microalgae using a hollow fiber membrane filter to maintain living cells; freezing the condensed microalgae; and microencapsulating the obtained unsaturated fatty acid. The hollow fiber membrane filter includes pores with a size smaller than 0.01micrometers.
Abstract translation: 目的：提供海洋养殖营养促进剂，促进贝类和鱼类养殖海洋微藻的生长，增强贝类和鱼类的免疫功能。 构成：通过以下步骤生产海洋栽培营养增强剂：以1000万个细胞/ ml的密度培养微藻; 使用中空纤维膜过滤器浓缩培养的微藻以维持活细胞; 冷冻浓缩微藻; 并将所得的不饱和脂肪酸微胶囊化。 中空纤维膜过滤器包括尺寸小于0.01微米的孔。
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公开(公告)号：KR1020090013045A
公开(公告)日：2009-02-04
申请号：KR1020080071371
申请日：2008-07-22
Applicant: 전남대학교산학협력단 , 김재민
Abstract: A nutrition enriched medium for the marine microalgae is provided to reduce the culture period of microalgae and improve the productivity by adding the material useful for growth acceleration and immunologic function reinforcement of the microalgae in the mass culture of the marine microalgae. The nutrition enriched medium for the marine microalgae is prepared by mixing the seawater with the agricultural fertilizer which contains 16.47-24.71 mg of nitrogen and 1.73-2.60 mg of phosphorus based on 1 liter of seawater and cytokinin which promotes the cell division of the marine microalgae.
Abstract translation: 提供海洋微藻营养富集培养基，通过在海洋微藻大规模培养中添加有益于微藻生长加速和免疫功能增强的材料，减少微藻的培养期，提高生产率。 通过将海水与含有16.47-24.71mg氮和1.73-2.60mg磷的农用肥料混合，制成海洋微藻的营养富集培养基，其中1升海水和细胞分裂素促进海洋微藻的细胞分裂 。
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公开(公告)号：KR1020110011022A
公开(公告)日：2011-02-08
申请号：KR1020090068453
申请日：2009-07-27
Applicant: 전남대학교산학협력단 , 김재민
Abstract: PURPOSE: An abalone feed using vegetable protein including attached diatoms and spirulina is provided to promote the growth of abalones, and to maintain body components of the abalones similar to natural abalones. CONSTITUTION: An abalone feed is produced by the following steps: cultivating attached diatoms and spirulina; drying the cultivated attached diatoms and spirulina; crushing the attached diatoms, the spirulina, the tangle weed, and brown-seaweed; and mixing the obtained ingredients. The attached diatoms, the spirulina, the tangle weed, and the brown-seaweed are mixed in a ratio of 20:10:35:35.
Abstract translation: 目的：提供使用植物蛋白（包括连接的硅藻和螺旋藻）的鲍鱼饲料，以促进鲍鱼的生长，并使鲍鱼的身体成分与天然的鲍鱼类似。 构成：通过以下步骤制备鲍鱼饲料：培养附着的硅藻和螺旋藻; 干燥栽培的硅藻和螺旋藻; 粉碎附着的硅藻，螺旋藻，杂草和褐藻; 并混合所得成分。 附着的硅藻，螺旋藻，杂草和褐藻都以20:10:35:35的比例混合。
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公开(公告)号：KR100461229B1
公开(公告)日：2004-12-14
申请号：KR1020020023881
申请日：2002-05-01
IPC: A01K61/00
CPC classification number: Y02A40/81
Abstract: 본 발명은 개불 성체가 들어있는 수조의 온도를 평소보다 3~5℃ 올리고 동시에 식물성 플랑크톤의 농도를 평소보다 높여서 산란을 유발하는 단계와, 상기 수정된 알과 정자를 세란하는 단계와, 상기 수정란을 분조하여 지수상태에서 부화시키는 단계와, 상기 부화된 유생을 15~20℃의 사육수조에 넣고 30일정도 사육하여 치충을 얻는 단계와, 상기 치충을 저질이 충진된 수조에 넣어 성체가 될 때까지 사육하는 단계로 구성된 개불의 사육방법을 제공하기 위한 것으로, 본 발명은 인공으로 개불을 대량 생산가능하도록 하므로써 고부가가치 양식생물 생산에 의한 어민소득 증대를 꾀할 수 있고, 수산자원의 효율적인 이용은 물론 양식품종의 다변화에 의한 국제 경쟁력 향상을 기할 수 있으며, 또한 개불 치충을 오염된 연안 저질에 방류함으로써 오염된 연� � 간석지의 환경을 정화 및 개선하고 연안 생태계의 친환경적 복원이 가능하게 되는 매우 유용한 발명인 것이다.
Abstract translation: 目的：提供一种育种方法，通过满足单线草的栖息地和繁殖生态条件，能够大量生产单子叶植物人工种子。 组成：该方法包括以下步骤：通过将含有Uicchis unicinctus的水族箱的温度升高比通常高3至5℃并且使植物浮游生物的浓度比平常高来诱导产卵; 清洁受精卵和精子; 划分受精卵以在截断状态孵化; 将孵化的幼虫放入15-20摄氏度的饲养水族箱中并饲养相同的饲养约30天以获得幼嫩尿道线虫; 并将年轻的urechis unicinctus放入充满底部材料的水族箱中并饲养，直到年轻的urechis unicinctus成为成年。
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Patent Agency Ranking
公开(公告)号：KR1020030085682A
公开(公告)日：2003-11-07
申请号：KR1020020023881
申请日：2002-05-01
IPC: A01K61/00
CPC classification number: Y02A40/81 , A01K61/00 , A01K67/033
Abstract: PURPOSE: A method for rearing urechis unicinctus is provided to enable a mass production of artificial seeds of urechis unicinctus by satisfying the conditions of habitat and reproduction ecologies of urechis unicinctus. CONSTITUTION: The method comprises the steps of: inducing spawning by increasing the temperature of an aquarium containing urechis unicinctus by 3 to 5deg.C higher than usual and increasing the concentration of phyto plankton higher than usual; cleaning fertilized spawns and sperms; dividing the fertilized spawns to hatch the same in a cutoff state; putting the hatched larvae in a rearing aquarium of 15 to 20deg.C and rearing the same for about 30 days to obtain young urechis unicinctus; and putting the young urechis unicinctus in an aquarium filled with bottom materials and rearing the same until the young urechis unicinctus become adults.
Abstract translation: 目的：提供一种养殖ureus unicinctus的方法，通过满足urechis unicinctus的栖息地和繁殖生态条件，大规模生产ureus unicinctus的人造种子。 构成方法：该方法包括以下步骤：通过将含有urechis unicinctus的水族箱的温度提高3倍至5度以上，诱导产卵，并增加植物浮游生物的浓度高于平常水平; 清洁受精的产卵和精子; 将受精的产卵分为孵化状态，处于截止状态; 将孵出的幼虫置于15至20度的饲养水族箱中，并饲养大约30天以获得年轻的urechis unicinctus; 并将年轻的urechis unicinctus放在一个充满底部材料的水族箱中，养育一下，直到年轻的urechis unicinctus成为大人。
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公开(公告)号：KR101477899B1
公开(公告)日：2015-01-08
申请号：KR1020130031960
申请日：2013-03-26
Applicant: 전남대학교산학협력단
Abstract: 본 발명은 연안 갯벌에서 유기물 분해능이 매우 우수한 것으로 알려진 갯지렁이(
Perinereis aibuhitensis )에 내생하고 있는 미생물을 MA 배지에서 생장하여 나타난 집락(colony)의 색깔과 모양에 따라 선별한 31개의 집락을 별도로 선별하여 순수배양한 후 16S rRNA 염기서열에 기초하여 동정한 결과,
Bacillus 속에 포함되는 균주를 분리하였다. 갯지렁이로부터 분리하여 선별한 두
Bacillus 균주 CBW4와 EBW4는 단백질, 탄수화물, 지질 분해능이 뛰어나고 염분에 내성을 갖고 있으므로 유기물로 오염된 양식장 폐수 환경을 정화하는데 유용하게 쓰일 수 있을 것이다.Abstract translation: 本发明涉及一种泻湖（泻湖）
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公开(公告)号：KR1020140117747A
公开(公告)日：2014-10-08
申请号：KR1020130032144
申请日：2013-03-26
Applicant: 전남대학교산학협력단
IPC: C02F3/34
Abstract: According to the present invention, microbes which live in Perinereis aibuhitensis in coastal mud flats have been grown in an MA culture medium and colonies have appeared, wherein the Perinereis aibuhitensis is known to have excellent degradability of organic matter. Thirty-one colonies have been separately selected according to the colors and shapes of the colonies which have appeared in the MA culture medium. Strains belonging to Bacillus genus have been isolated as a result of identification on the basis of 16S rRNA sequences after a pure culture process of the 31 colonies. Bacillus strains EBW4 and CBW4 isolated from the Perinereis aibuhitensis have excellent degradabilities of protein, carbohydrate and lipid and have resistance to salt. Accordingly, the EBW4 and CBW4 can be practically used to purify the wastewater environment of a farm contaminated with organic matter.
Abstract translation: 根据本发明，在沿海泥浆平面上生活在围Per中的微生物已经在MA培养基中生长，出现菌落，其中已知百日蓟马具有优异的有机物降解性。 根据出现在MA培养基中的菌落的颜色和形状分别选择了三十一个菌落。 在31个菌落的纯培养过程之后，基于16S rRNA序列的鉴定结果已经分离了属于芽孢杆菌属的菌株。 从百日蓟马分离的芽孢杆菌菌株EBW4和CBW4具有优异的蛋白质，碳水化合物和脂质的降解性，具有耐盐性。 因此，EBW4和CBW4实际上可用于净化有机物污染的农场的废水环境。
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公开(公告)号：KR1020140117732A
公开(公告)日：2014-10-08
申请号：KR1020130031984
申请日：2013-03-26
Applicant: 전남대학교산학협력단
CPC classification number: C02F3/341 , C02F2101/30 , C12N1/20
Abstract: The present invention separated a strain included in Bacillus sp. from the result of identification based on base sequence 16S rRNA after pure cultivating, by separately selecting thirty one colonies selected according to colors and shapes of colonies, generated by growing microorganism, arbuscular, in perinereis aibuhitensis known to have excellent ability of decomposing organic matters in mud flat along the shore, in MA medium. A strain CBW4 selected by being separated from perinereis aibuhitensis can be usefully used in purifying the environment filled with waste water in a farm contaminated due to the organic matters, by having salinity tolerance and excellent ability of decomposing protein, carbohydrate and lipid.
Abstract translation: 本发明分离芽孢杆菌属菌株中包含的菌株。 从纯培养后的基因序列16S rRNA的鉴定结果，通过分离选择根据生长的微生物生长的菌落的颜色和形状选择的三十一个菌落，已知具有优异的分解有机物质的能力的围is 沿岸平坦，MA中。 通过从围is中分离出来的菌株CBW4可以有效地用于净化由于有机物污染的农场中充满废水的环境，具有耐盐性和分解蛋白质，碳水化合物和脂质的优异能力。
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公开(公告)号：KR101414361B1
公开(公告)日：2014-07-01
申请号：KR1020120129282
申请日：2012-11-15
Applicant: 전남대학교산학협력단
Abstract: 본 발명은 연안 갯벌에서 유기물 분해능이 매우 우수한 것으로 알려진 갯지렁이(
Perinereis aibuhitensis )에 내생하고 있는 미생물을 MA 배지에서 생장하여 나타난 집락(colony)의 색깔과 모양에 따라 선별한 31개의 집락을 별도로 선별하여 순수배양한 후 16S rRNA 염기서열에 기초하여 동정한 결과,
Bacillus 속에 포함되는 11 균주를 분리하였다.
Bacills 속(genus)에 포함되는 11 균주의 다양성을 분석한 결과
Bacills algicola, Bacills anthrasis, Bacills aquimaris, Bacills barbaricus, Bacills hwajinpoensis, Bacills nanhiensis, Bacills vietnamensis 등 모두 7 종 (species)의
Bacillus spp.가 갯지렁이(
Perinereis aibuhitensis )에 내생하는 것으로 조사되었다. 본 발명에서 갯지렁이로부터 분리하여 선별한 두
Bacillus 균주들은 단백질, 탄수화물, 지질 분해능이 뛰어나고 염분에 내성을 갖고 있으므로 유기물로 오염된 갯벌 환경을 정화하는데 유용하게 쓰일 수 있을 것이다.Abstract translation: 本发明涉及一种泻湖（泻湖）
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