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    USE OF DIMETHYL DISULFIDE FOR METHIONINE PRODUCTION IN MICROORGANISMS
    4.
    发明申请
    USE OF DIMETHYL DISULFIDE FOR METHIONINE PRODUCTION IN MICROORGANISMS 审中-公开
    二甲基磺脲在微生物中的生产中的应用

    公开(公告)号：WO2007011939A3

    公开(公告)日：2007-07-05

    申请号：PCT/US2006027855

    申请日：2006-07-18

    Applicant: BASF AG ,  ZELDER OSKAR ,  HAEFNER STEFAN ,  HEROLD ANDREA ,  KLOPPROGGE CORINNA ,  SCHRODER HARTWIG ,  YOCUM R ROGERS ,  WILLIAMS MARK K

    Inventor： ZELDER OSKAR ,  HAEFNER STEFAN ,  HEROLD ANDREA ,  KLOPPROGGE CORINNA ,  SCHRODER HARTWIG ,  YOCUM R ROGERS ,  WILLIAMS MARK K

    IPC: C12P13/12

    CPC classification number: C12P13/12

    Abstract: The present invention features improved processes and organisms for the production of methionine. The invention demonstrates that a ?metF organism or a ?metE AmetH organism, for example, mutants of C. glutamicum or E. coli, can use a methyl capped sulfide source, e.g., dimethyl disulfide (DMDS), as a source of both sulfur and a methyl group, bypassing the need for MetH/Met? and MetF activity and the need to reduce sulfate, for the synthesis of methionine. Also described in this patent are data implicating MetY (also called MetZ) as an enzyme that incorporates a methyl capped sulfide source, e.g., DMDS, into methionine. A ?metF ?metB strain of C. glutamicum can use a methyl capped sulfide source, e.g., DMDS, as a source of both sulfide and a methyl group. Furthermore, methionine production by engineered prototrophic organisms that overproduce O-acetyl-homoserine was improved by the addition of a methyl capped sulfide source, e.g., DMDS.

    Abstract translation: 本发明的特征在于用于生产甲硫氨酸的改进方法和生物体。 本发明证明，metF生物体或αmetE AmetH生物体，例如谷氨酸棒状杆菌或大肠杆菌的突变体可以使用甲基封端的硫化物源，例如二甲基二硫（DMDS）作为硫的来源 和甲基，绕过MetH / Met？ 和MetF活性以及减少硫酸盐的需要，用于合成甲硫氨酸。 该专利中还描述了涉及MetY（也称为MetZ）的数据，其作为将甲基封端的硫化物源（例如DMDS）并入甲硫氨酸中的酶。 谷氨酸棒状芽孢杆菌的ΔFIFFmetB菌株可以使用甲基封端的硫化物源，例如DMDS，作为硫化物和甲基两者的来源。 此外，通过加入甲基封端的硫化物源（例如DMDS），改善过度产生O-乙酰高丝氨酸的工程化原养殖生物体的甲硫氨酸生产。

    6.
    发明专利
    未知

    公开(公告)号：BRPI0519165A2

    公开(公告)日：2008-12-30

    申请号：BRPI0519165

    申请日：2005-12-21

    Applicant: BASF AG

    Inventor： HAFNER STEFAN ,  KLOPPROGGE CORINNA ,  HEROLD ANDREA ,  SCHROEDER HARTWIG ,  ZELDER OSKAR

    IPC: C12N15/77 ,  C07K14/34

    Abstract: An expression unit (A) that contains several promoters is new. A new expression unit (A) comprises several promoters and includes, in the 5' to 3' direction the sequence module (I). 5'-P 1-(A x-P x) n-A y-P y-3' (I) n = integer 0-10; A x and A y = same or different chemical bonds or nucleic acid linker sequences; P 1, P x and P y = same or different promoter sequences, containing at least one RNA-polymerase binding region, and at least P y also includes a ribosome-binding, 3'-terminal segment. Independent claims are also included for: (1) an expression cassette (EC) that contains, in the 5' to 3' direction, the sequence module (II); (2) vector that contains at least one EC; (3) a genetically modified microorganism (GMO) transfected with the vector of (2) or containing EC; and (4) method for preparing biosynthetic products (X) by culturing GMO. 5'-P 1-(A x-P x) n-A y-P y-G-3' (II) G : at least one nucleic acid coding sequence functionally linked to the 5'-upstream regulatory sequence.

    USE OF DIMETHYL DISULFIDE FOR METHIONINE PRODUCTION IN MICROORGANISMS
    8.
    发明专利
    USE OF DIMETHYL DISULFIDE FOR METHIONINE PRODUCTION IN MICROORGANISMS 未知

    公开(公告)号：CA2615315A1

    公开(公告)日：2007-01-25

    申请号：CA2615315

    申请日：2006-07-18

    Applicant: BASF AG

    Inventor： YOCUM R ROGERS ,  SCHRODER HARTWIG ,  KLOPPROGGE CORINNA ,  HAEFNER STEFAN ,  HEROLD ANDREA ,  WILLIAMS MARK K ,  ZELDER OSKAR

    IPC: C12P13/12

    Abstract: The present invention features improved processes and organisms for the production of methionine. The invention demonstrates that a &Dgr;metF organism or a &Dgr;metE AmetH organism, for example, mutants of C. glutamicum or E. coli, can use a methyl capped sulfide source, e.g., dimethyl disulfide (DMDS), as a source of both sulfur and a methyl group, bypassing the need for MetH/MetE and MetF activity and the need to reduce sulfate, for the synthesis of methionine. Also described in this patent are data implicating MetY (also called MetZ) as an enzyme that incorporates a methyl capped sulfide source, e.g., DMDS, into methionine. A &Dgr;metF &Dgr;metB strain of C. glutamicum can use a methyl capped sulfide source, e.g., DMDS, as a source of both sulfide and a methyl group. Furthermore, methionine production by engineered prototrophic organisms that overproduce O-acetyl-homoserine was improved by the addition of a methyl capped sulfide source, e.g., DMDS.

    MULTIPLE PROMOTERS AND THE USE THEREOF FOR GENE EXPRESSION
    9.
    发明专利
    MULTIPLE PROMOTERS AND THE USE THEREOF FOR GENE EXPRESSION 未知

    公开(公告)号：CA2591311A1

    公开(公告)日：2006-07-06

    申请号：CA2591311

    申请日：2005-12-21

    Applicant: BASF AG

    Inventor： HAEFNER STEFAN ,  HEROLD ANDREA ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG

    IPC: C12N15/77 ,  C07K14/34

    Abstract: An expression unit (A) that contains several promoters is new. A new expression unit (A) comprises several promoters and includes, in the 5' to 3' direction the sequence module (I). 5'-P 1-(A x-P x) n-A y-P y-3' (I) n = integer 0-10; A x and A y = same or different chemical bonds or nucleic acid linker sequences; P 1, P x and P y = same or different promoter sequences, containing at least one RNA-polymerase binding region, and at least P y also includes a ribosome-binding, 3'-terminal segment. Independent claims are also included for: (1) an expression cassette (EC) that contains, in the 5' to 3' direction, the sequence module (II); (2) vector that contains at least one EC; (3) a genetically modified microorganism (GMO) transfected with the vector of (2) or containing EC; and (4) method for preparing biosynthetic products (X) by culturing GMO. 5'-P 1-(A x-P x) n-A y-P y-G-3' (II) G : at least one nucleic acid coding sequence functionally linked to the 5'-upstream regulatory sequence.

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