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    2.
    发明专利
    未知

    公开(公告)号：DE10315760A1

    公开(公告)日：2004-10-21

    申请号：DE10315760

    申请日：2003-04-07

    Applicant: BASF AG

    Inventor： ALTHOEFER HENNING ,  KESELER MARIA

    IPC: C12N9/04 ,  C12N15/53 ,  C12P7/04 ,  C12P13/00 ,  C12P17/00 ,  C12P41/00 ,  C12P7/02

    Abstract: The present invention relates to proteins having an enzymatic activity of reducing substituted alkanones such as 3-methylamino-1-(2-thienyl)-propan-1-one. The invention furthermore relates to nucleic acids coding for said proteins, nucleic acid constructs, vectors, genetically modified microorganisms and to methods for preparing substituted (S)-alkanols, such as, for example, (S)-3-methylamino-1-(2-thienyl)-(S)-propanol.

    Preparation of optically active alkanol compound, useful for the treatment of depression or incontinence, comprises incubating an enzyme with a polypeptide sequence in a medium containing alkanone compound and isolating
    3.
    发明专利
    Preparation of optically active alkanol compound, useful for the treatment of depression or incontinence, comprises incubating an enzyme with a polypeptide sequence in a medium containing alkanone compound and isolating 未知

    公开(公告)号：DE102005062662A1

    公开(公告)日：2007-06-28

    申请号：DE102005062662

    申请日：2005-12-23

    Applicant: BASF AG

    Inventor： STUERMER RAINER ,  KESELER MARIA ,  HAUER BERNHARD ,  FRIEDRICH THOMAS ,  BREUER MICHAEL ,  SCHROEDER HARTWIG

    IPC: C12N9/04 ,  C12P7/02

    Abstract: Preparation of optically active alkanol compound (I) comprises incubating an enzyme with a polypeptide sequence of SEQ ID NO:1 (Not defined) or the SEQ ID NO:1 with up to 25% of amino acids modified by deletion, insertion and/or substitution and at least 50% of which possess enzymatic activity, in a medium containing alkanone compound (II) and isolating the product, where (II) is enzymatically reduced to (I). Preparation of optically active alkanol compound of formula (I) comprises incubating an enzyme with a polypeptide sequence of SEQ ID NO:1 (Not defined) or the SEQ ID NO:1 with up to 25% of amino acids modified by deletion, insertion and/or substitution and at least 50% of which possess enzymatic activity, in a medium containing alkanone compound of formula Cyc-(C=O)-(CH 2) n-CH 2-R 1>(II) and isolating the product, where (II) is enzymatically reduced to (I). n : 0-5; Cyc : optionally substituted, mono- or polynuclear, (un)saturated, carbocylic or heterocyclic ring; R 1>halo, SH, OH, NO 2, NR 2>R 3> or NR 2>R 3>R 4+>X ->; R 2>-R 4>H, lower alkyl or lower alkoxy; and X ->counter ion. An independent claim is also included for the enzyme with dehydrogenase activity. [Image] ACTIVITY : Antidepressant; Uropathic. No biological data given. MECHANISM OF ACTION : None given.

    6.
    发明专利
    未知

    公开(公告)号：DE102005044736A1

    公开(公告)日：2007-03-22

    申请号：DE102005044736

    申请日：2005-09-19

    Applicant: BASF AG

    Inventor： BREUER MICHAEL ,  FRIEDRICH THOMAS ,  KESELER MARIA

    IPC: C12N9/04 ,  C12N15/53 ,  C12N15/63 ,  C12P7/02 ,  C12P7/22 ,  C12P17/00

    Abstract: The present invention relates to proteins having an enzymatic activity of reducing substituted alkanones such as 3-methylamino-1-(2-thienyl)-propan-1-one. The invention furthermore relates to nucleic acids coding for said proteins, nucleic acid constructs, vectors, genetically modified microorganisms and to methods for preparing optically active substituted alkanols, such as, for example, (S)-3-methylamino-1-(2-thienyl)-(S)-propanol.

    8.
    发明专利
    未知

    公开(公告)号：DE102004022686A1

    公开(公告)日：2005-11-24

    申请号：DE102004022686

    申请日：2004-05-05

    Applicant: BASF AG

    Inventor： STUERMER RAINER ,  KESELER MARIA ,  HAUER BERNHARD ,  FRIEDRICH THOMAS ,  BREUER MICHAEL ,  SCHROEDER HARTWIG

    IPC: C07D333/16 ,  C12N9/04 ,  C12P20060101 ,  C12P7/02 ,  C12P13/00 ,  C12P17/00

    Abstract: The invention relates to a method for producing the optically active alkanols of formula (I), wherein n is an integer of from 0 to 5; Cyc represents an optionally substituted, mononuclear or polynuclear, saturated or unsaturated, carbocylic or heterocyclic ring, and R1 represents halogen, SH, OH, NO2, NR2R3 or NR2R3R4+X−, wherein R2, R3 and R4 independently represent H or a lower alkyl or lower alkoxy group and X− represents a counterion. According to the invention, an enzyme (E) selected from the groups of dehydrogenases, aldehyde reductases and carbonyl reductases is incubated in a medium containing the alkanone of formula (II), wherein n, Cyc and R1 are defined as above, in the presence of reduction equivalents. The compound of formula (II) is enzymatically reduced to the compound of formula (I) and the reduction equivalents consumed during reaction are regenerated by reacting a sacrificial alcohol to the corresponding sacrificial ketone using enzyme (E) and at least partially removing the sacrificial ketone from the reaction medium, and then isolating the product (I) so produced.

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