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    TRANSFECTION IN MAGNETICALLY DRIVEN CONTINUOUS FLOW
    1.
    发明申请
    TRANSFECTION IN MAGNETICALLY DRIVEN CONTINUOUS FLOW 审中-公开
    磁力传递连续流量的转移

    公开(公告)号：WO2008100749A3

    公开(公告)日：2008-11-20

    申请号：PCT/US2008053135

    申请日：2008-02-06

    Applicant: BIO RAD LABORATORIES ,  RAGSDALE CHARLES W ,  RUBIO TERESA

    Inventor： RAGSDALE CHARLES W ,  RUBIO TERESA

    IPC: A61K48/00 ,  C07H21/04 ,  C12N15/87

    CPC classification number: C12N13/00 ,  C12M35/02 ,  C12M35/06 ,  C12N15/85 ,  C12N15/87

    Abstract: Biological cells and other membranous structures are transfected in a flow-through system by first rendering the structures magnetically active such that they respond to a magnetic field, suspending the structures in a solution of an exogenous species with which the structures are to be transfected, then placing the suspension in a channel and using a moving magnetization pattern along the channel wall to cause the structures to travel through the channel. Along their path of travel, the structures pass a transmitter that emits transfection energy sufficient to cause the exogenous species in the suspension to permeate the structure membranes and enter the interiors of the structures.

    Abstract translation: 生物细胞和其他膜结构在流通系统中转染，首先使结构具有磁性活性，使得它们响应于磁场，将结构悬浮在结构待转染的外源物质的溶液中，然后 将悬架放置在通道中并且沿着通道壁使用移动的磁化模式以使结构行进通过通道。 沿着他们的旅行路线，结构通过发射器，其发射足够的转染能量，使得悬浮液中的外源物质渗入结构膜并进入结构的内部。

    RESISTOR PULSE MODULATION
    2.
    发明申请
    RESISTOR PULSE MODULATION 审中-公开
    电阻器脉冲调制

    公开(公告)号：WO2008036800A3

    公开(公告)日：2008-11-13

    申请号：PCT/US2007078997

    申请日：2007-09-20

    Applicant: BIO RAD LABORATORIES ,  RAGSDALE CHARLES W

    Inventor： RAGSDALE CHARLES W

    IPC: H02J7/00

    CPC classification number: C12N13/00 ,  A61N1/327 ,  C12M35/02 ,  H03K3/53

    Abstract: Systems, methods, apparatus, and circuits for controlling an electrical signal transmitted to a sample load are provided. The electrical signal produced by a capacitor is controlled via a control signal sent to a variable resistance device that is connected in parallel with the sample load. The variable resistance device includes a resistance and a switch in series. The control signal opens and closes the switch, thus providing a variable resistance based on the amount of time the switch is closed.

    Abstract translation: 提供了用于控制传送到样本负载的电信号的系统，方法，装置和电路。 由电容器产生的电信号通过发送到与样品负载并联连接的可变电阻器件的控制信号来控制。 可变电阻器件包括串联的电阻和开关。 控制信号打开和​​关闭开关，从而根据开关闭合的时间量提供可变电阻。

    TRANSFECTION IN ELECTRONICALLY DRIVEN CONTINUOUS FLOW
    5.
    发明申请
    TRANSFECTION IN ELECTRONICALLY DRIVEN CONTINUOUS FLOW 审中-公开
    电子驱动连续流中的转移

    公开(公告)号：WO2008112030A3

    公开(公告)日：2008-11-06

    申请号：PCT/US2007084428

    申请日：2007-11-12

    Applicant: BIO RAD LABORATORIES ,  RAGSDALE CHARLES W

    Inventor： RAGSDALE CHARLES W

    IPC: C12N13/00 ,  C12N15/87

    CPC classification number: C12N15/87 ,  C12M35/02

    Abstract: Biological cells and other membranous structures are transfected in a flow-through system by using a moving charge pattern on a longitudinal wall of a channel to cause the cells to travel through the channel due to an electrostatic interaction between the cells and the moving charge pattern. As the cells travel through the channel, they pass a transmitter that emits transfection energy sufficient to make the cell membranes permeable such that exogenous species in the fluid in which the cells are suspended will enter the cell interiors.

    Abstract translation: 通过在通道的纵向壁上使用移动的电荷图案将生物细胞和其它膜结构转染到流通系统中，以使得细胞由于细胞与移动的电荷模式之间的静电相互作用而穿过通道。 当细胞穿过通道时，它们通过发射器，其发射足以使细胞膜渗透的转染能量，使得悬浮细胞的流体中的外源物质将进入细胞内部。

    APPARATUS FOR HIGH-THROUGHPUT ELECTROPORATION
    6.
    发明申请
    APPARATUS FOR HIGH-THROUGHPUT ELECTROPORATION 审中-公开
    高通电气设备

    公开(公告)号：WO2007094947A2

    公开(公告)日：2007-08-23

    申请号：PCT/US2007002327

    申请日：2007-01-26

    Applicant: BIO RAD LABORATORIES ,  RAGSDALE CHARLES W ,  FULLER JOHN M ,  STEPHENS NICHOLAS R

    Inventor： RAGSDALE CHARLES W ,  FULLER JOHN M ,  STEPHENS NICHOLAS R

    CPC classification number: C12M35/02 ,  C12M23/12

    Abstract: An electroporator for high-throughput electroporation is constructed with a well plate in which each well has internal electrodes that extend beyond the opening of the well to form contact areas, either as horizontal platforms extending laterally from the well rims or as extended heights of thin electrode plates. The electroporator also includes a lid that contains circuitry and electrical contacts that mate with the exposed contact areas in the well plate. The interchangeability of lids allows the wells to be shocked according to different protocols.

    Abstract translation: 用于高通量电穿孔的电穿孔机构造有井板，其中每个井具有延伸超过井的开口的内部电极以形成接触区域，或者作为从井壁横向延伸的水平平台或者作为薄电极的扩展高度 板。 电动机还包括盖子，其包含与孔板中暴露的接触区域相配合的电路和电触点。 盖子的互换性允许孔根据不同的方案而受到冲击。

    ELECTROPORATION CUVETTE INSERT FOR FACILITATING MEMBRANE-BASED FUSION
    7.
    发明专利
    ELECTROPORATION CUVETTE INSERT FOR FACILITATING MEMBRANE-BASED FUSION 未知

    公开(公告)号：CA2437062C

    公开(公告)日：2009-04-21

    申请号：CA2437062

    申请日：2002-12-06

    Applicant: BIO RAD LABORATORIES

    Inventor： RAGSDALE CHARLES W

    IPC: C12M1/00 ,  C12M1/42 ,  C12M3/00 ,  C12N13/00 ,  C12N15/02

    Abstract: Cuvette inserts adapted and configured to fit within an electroporation cuvette. The inserts each include a support structure that holds a porous membrane. When positioned within the cuvette, the membrane is positioned proximal the cuvette electrodes to facilitate membrane-based fusion of cells . In certain aspects, a tube extends through the support structure to allow fo r application of negative pressure in a convenient location away from electrod e contacts and other components of the cuvette or cuvette holder.

    TRANSFECTION IN MAGNETICALLY DRIVEN CONTINUOUS FLOW
    8.
    发明专利
    TRANSFECTION IN MAGNETICALLY DRIVEN CONTINUOUS FLOW 未知

    公开(公告)号：CA2677171A1

    公开(公告)日：2008-08-21

    申请号：CA2677171

    申请日：2008-02-06

    Applicant: BIO RAD LABORATORIES

    Inventor： RUBIO TERESA ,  RAGSDALE CHARLES W

    IPC: A61K48/00 ,  C07H21/04 ,  C12N15/87

    Abstract: Biological cells and other membranous structures are transfected in a flo w-through system by first rendering the structures magnetically active such that they respond to a magnetic field, suspending the structures in a soluti on of an exogenous species with which the structures are to be transfected, then placing the suspension in a channel and using a moving magnetization pa ttern along the channel wall to cause the structures to travel through the c hannel. Along their path of travel, the structures pass a transmitter that e mits transfection energy sufficient to cause the exogenous species in the su spension to permeate the structure membranes and enter the interiors of the structures.

    ELECTROPORATION CELL WITH ARC PREVENTION OR REDUCTION
    9.
    发明专利
    ELECTROPORATION CELL WITH ARC PREVENTION OR REDUCTION 未知

    公开(公告)号：CA2375362C

    公开(公告)日：2006-10-31

    申请号：CA2375362

    申请日：2000-05-09

    Applicant: BIO RAD LABORATORIES

    Inventor： RAGSDALE CHARLES W ,  TAGLIAMONTE JOHN

    IPC: C12M1/00 ,  C12N13/00 ,  A61N1/08 ,  A61N1/30 ,  C12M1/42 ,  C12N15/64 ,  G01N37/00

    Abstract: An electroporation system and method for directing high-voltage currents to a suspension of cells contained in a cuvette. A high-voltage switch controls t he coupling of a charge control to a high-voltage capacitor and, additionally, controls the coupling of the high-voltage capacitor to the cuvette. A curren t diverter diverts current away from the sample whenever an arc condition commences or a low sample resistance is detected across the cuvette. The current diverter includes a current diverter switch, which is triggered when a sense resistor measures a second predetermined voltage indicative of an arc- over event (or a low sample resistance condition).

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