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91.发明公开
公开(公告)号：KR1020140081447A
公开(公告)日：2014-07-01
申请号：KR1020120151209
申请日：2012-12-21
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: The present invention relates to an expression vector comprising a gene encoding a prion protein of Capra hircus and a cell transduced by the vector. The present invention can be used for manufacturing a cell having polymorphism, which represents Capra hircus bred in Korea, and a transduced mouse; and can be effectively used for developing an animal experimental model for studies including diagnosis of prion diseases which include scrapie occurred in goat family animals, and for confirming if domestic TSE diseases interspecifically spread or not.
Abstract translation: 本发明涉及一种表达载体，其包含编码Capra hircus的朊病毒蛋白的基因和由载体转导的细胞。 本发明可用于制造具有多形性的细胞，其代表在韩国繁殖的Capra hircus和转导的小鼠; 可以有效地用于研究包括诊断朊病毒疾病的动物实验模型，包括山羊家族动物发生的瘙痒病，以及确认国内TSE疾病是否特异性传播。
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公开(公告)号：KR1020120086024A
公开(公告)日：2012-08-02
申请号：KR1020110007213
申请日：2011-01-25
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: C12Q1/045 , C12N7/00 , G01N31/221 , G01N33/52
Abstract: PURPOSE: A medium for maintainig and determining west nile is provided to reduce the exposure by high risk virus and to shorten the use of harmful reagents. CONSTITUTION: A medium for maintaining and detecting west nile contains alpha-MEM or DMEM containing 1-10 g/L of glucose and pH indicator. The pH indicator is phenol red. A potency assay method for detecting west nile comprises: a step of culturing west nile host cells and a sample in the medium; and a step of measuring the pH concentration of the medium to detecting west nile virus and tittering west nile in a sample. The host cells are monkey kidney cells.
Abstract translation: 目的：提供维护和确定西尼罗河的介质，以减少高风险病毒的暴露并缩短有害试剂的使用。 构成：用于维持和检测西尼罗河的培养基含有含有1-10克/升葡萄糖和pH指示剂的α-MEM或DMEM。 pH指示剂为酚红。 用于检测西尼罗河的效力测定方法包括：培养西尼罗河宿主细胞和培养基中的样品的步骤; 以及测定介质中检测西尼罗病毒并滴定样品中西尼罗河的pH浓度的步骤。 宿主细胞是猴肾细胞。
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93.发明公开
公开(公告)号：KR1020120075678A
公开(公告)日：2012-07-09
申请号：KR1020100137439
申请日：2010-12-29
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: A61K36/73 , A23V2200/322 , A61K2236/331 , A61K2236/333
Abstract: PURPOSE: A composition containing Rubi Fructus extract is provided to inhibit modified prion production and to prevent or treat BSE. CONSTITUTION: A veterinary composition for preventing or treating BSE contains Rubi Fructus extract. The extract is prepared using water, low alcohol of C1-C4, and a mixture solvent thereof. The concentration of the extract is 6.25ug/ml. The daily dose of the composition is 0.0001-100 mg/kg. A feed additive composition for preventing or treating BSE contains the extract. A feed contains the extract.
Abstract translation: 目的：提供含有Rubi Fructus提取物的组合物，以抑制修饰的朊病毒生成并预防或治疗BSE。 组成：用于预防或治疗疯牛病的兽用组合物含有Rubi Fructus提取物。 提取物是使用水，C1-C4的低级醇及其混合溶剂制备的。 提取物的浓度为6.25ug / ml。 组合物的日剂量为0.0001-100mg / kg。 用于预防或治疗BSE的饲料添加剂组合物含有提取物。 Feed包含提取物。
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Patent Agency Ranking94.发明授权소코로나바이러스, 소로타바이러스, 및 소바이러스성설사병바이러스를 동시에 검출할 수 있는 프라이머 및 이를 이용한 소 등 반추류의 설사유발 바이러스 동시 검출법 有权能够同时检测牛冠状病毒，牛轮状病毒和牛病毒性腹泻病毒的引物，以及反刍动物（包括牛）中的腹泻病毒的同时检测方法，使用该引物
公开(公告)号：KR101064866B1
公开(公告)日：2011-09-15
申请号：KR1020080116959
申请日：2008-11-24
Applicant: 대한민국(농림축산식품부 농림축산검역본부장) , 주식회사 인트론바이오테크놀로지
CPC classification number: Y02A50/451
Abstract: 본 발병은 소 및 반추류에서 설사를 유발하는 주요 바이러스인 소코로나바이러스, 소로타바이러스, 및 소바이러스성설사병바이러스를 동시에 감별 검출할 수 있는 검출 방법에 관한 것으로, 보다 상세하게는 역전사 반응 및 PCR로 구성된 방법을 통하여 상기 바이러스들을 동시에 검출 및 감별할 수 있는 데에 활용될 수 있는 특이 프라미어들과, 이들 프라이머들을 이용한 상기 소 설사 유발 바이러스들을 동시에 감별 검출할 수 있는 PCR 검출 방법에 관한 것이다. 본 발명은 소 및 반추류의 코로나바이러스, 로타바이러스, 및 소바이러스성설사병바이러스의 감별 검출이 가능하여 일차적으로 상기 바이러스들의 감염 진단 및 이들 바이러스들에 의해 유발되는 질환의 감별 진단에 효과적으로 활용될 수 있으며, 또한 상기 바이러스들의 감염 진단 키트로도 구현이 가능하다.
소, 반추류, 설사, 바이러스, 코로나바이러스, 로타바이러스, 소바이러스성설사병바이러스, 페스티바이러스, 검출, 감별, 질환, 동시, 진단, 프라이머, PCR-
公开(公告)号：KR1020110009976A
公开(公告)日：2011-01-31
申请号：KR1020090067453
申请日：2009-07-23
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: PURPOSE: A monoclonal antibody for specifically identifying and detecting abnormal prion of chronic wasting disease is provided. CONSTITUTION: A fusion cell line of deposit number KCLRF-BP-00214 produces a monoclonal antibody for detecting abnormal prion of chronic wasting disease. A method for preparing fusion cell line comprises: a step of inoculating a peptide of sequence number 1 to knock-out mouse; a step of isolating B cells from the mouse; and a step of fusing the B cells with mouse myeloma cells. The monoclonal antibody is used for detecting the abnormal prion through western blotting.
Abstract translation: 目的：提供用于特异性鉴定和检测慢性消耗性疾病异常朊病毒的单克隆抗体。 构成：融合细胞系KCLRF-BP-00214产生一种用于检测慢性消耗性疾病异常朊病毒的单克隆抗体。 制备融合细胞系的方法包括：将序列号1的肽接种到敲除小鼠的步骤; 从小鼠中分离出B细胞的步骤; 以及将B细胞与小鼠骨髓瘤细胞融合的步骤。 单克隆抗体用于通过Western印迹检测异常朊病毒。
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公开(公告)号：KR1020100121288A
公开(公告)日：2010-11-17
申请号：KR1020090040368
申请日：2009-05-08
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: PURPOSE: A vaccine virus for swine fever of gene recombination is provided to easily detect mutation of vaccine virus and to early remove infected pig. CONSTITUTION: A vaccine virus for swine fever, Flc-LOM-BErns virus(KFCC11442P) contains a nucleotide of sequence number 1. An Erns gene of CSFV(classical swine fever virus) is substituted with Erns gene of BVDV(bovine viral disease virus) in the vaccine virus. The vaccine virus is Erns gene marker, Erns serologic marker or END(Exaltaton of Newcastle Disease virus) bio marker. A method for manufacturing swine vaccine virus, Flc-LOM-BErns virus(KFCC11442P) comprises: a step of extracting genomic RNA from swine fever virus strain; a step of synthesizing full-length cDNA to total RNA; a step of removing Erns gene from the synthesized cDNA; and a step of inserting Erns gene of BVDV.
Abstract translation: 目的：提供用于猪瘟基因重组的疫苗病毒，以便于检测疫苗病毒的突变并及早清除感染猪。 构成：用于猪瘟的疫苗病毒Flc-LOM-BErns病毒（KFCC11442P）含有序列号1的核苷酸。将CSFV（经典猪瘟病毒）的Erns基因用BVDV（牛病毒病毒）的Erns基因取代， 在疫苗病毒中。 疫苗病毒是Erns基因标记，Erns血清学标记或END（新城疫病毒Exaltaton）生物标志物。 一种制造猪疫苗病毒Flc-LOM-BErns病毒（KFCC11442P）的方法包括：从猪瘟病毒株中提取基因组RNA的步骤; 将全长cDNA合成到总RNA中的步骤; 从合成的cDNA中除去Erns基因的步骤; 和插入BVDV的Erns基因的步骤。
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公开(公告)号：KR100948127B1
公开(公告)日：2010-03-18
申请号：KR1020070063532
申请日：2007-06-27
Applicant: 주식회사 중앙백신연구소 , 대한민국(농림축산식품부 농림축산검역본부장)
IPC: A61K39/145 , A61K39/12 , A61P11/00
Abstract: 본 발명은 돼지 복합호흡기 질환의 치료 및 예방을 위한 백신에 관한 것이다. 본 발명의 따르면, 복합호흡기 질환에 걸린 돼지의 감염 조직으로부터 항원을 추출하여 백신으로 이용한다. 이와 같이 제조한 본 발명의 백신은 안정성 및 효과에 있어 기존의 백신과 비교할 수 없는 현저히 우수한 효과를 갖는다.
감염조직백신, 이유후전신소모성증후군(PMWS), 돼지호흡기복합증후군(PRDC)-
98.发明公开
公开(公告)号：KR1020040095824A
公开(公告)日：2004-11-16
申请号：KR1020030026809
申请日：2003-04-28
Applicant: 대한민국(농림축산식품부 농림축산검역본부장) , 프린스톤바이오메디테크코포레이션 , (주)피비엠이스트
Inventor： 조인수 , 현방훈 , 이광녕 , 엄재구 , 계수정 , 고영준 , 구복경 , 주이석 , 안수환 , 김인중 , 김옥경 , 김희정 , 장기용 , 신남규 , 황서하 , 강제모 , 김창호 , 고송우
IPC: G01N33/543
CPC classification number: G01N33/56983 , G01N33/526 , G01N33/558 , G01N2333/09 , Y10S436/811
Abstract: PURPOSE: A method for diagnosis of foot and mouth disease and a diagnostic kit are provided, thereby rapidly and accurately diagnosing foot and mouth disease with a small amount of a sample, and easily and rapidly diagnosing foot and mouth disease of domestic animals in the field. CONSTITUTION: The method for diagnosis of foot and mouth disease comprises the steps of: (1) introducing a testing sample into a loading area of a strip; (2) combining a detecting reagent with a marker with a target material in the sample to prepare a complex; (3) developing the complex on a membrane; and (4) measuring the appearance change of a reaction portion having one or more fixing phase selected from antigen, antibody or hapten which is obtainable through the immune reaction from FMDV in a certain region of the membrane. The diagnostic kit comprises a reaction portion(13) having one or more fixing phase selected from antigen, antibody or hapten which is obtainable through the immune reaction from FMDV; a strip(1') having a control portion for determining normal operation on a certain region; a testing sample introducing portion; and a housing with a displaying window(4) for displaying reaction results in the reaction portion(13) and the control portion. on the strip(1').
Abstract translation: 目的：提供口蹄疫诊断方法和诊断试剂盒，用少量样品快速，准确地诊断口蹄疫，容易快速诊断现场家畜的口蹄疫 。 规定：口蹄疫诊断方法包括以下步骤：（1）将检测样品引入条带的装载区域; （2）将检测试剂与标记物与样品中的靶材料组合以制备复合物; （3）在膜上显影复合物; 和（4）测量具有一个或多个定影相的反应部分的外观变化，所述固定相选自抗原，抗体或半抗原，所述抗原，抗体或半抗原可通过膜的某一区域中的FMDV通过免疫反应获得。 诊断试剂盒包括具有选自抗原，抗体或半抗原的一个或多个固定相的反应部分（13），其可通过FMDV的免疫反应获得; 具有用于确定某一区域正常操作的控制部分的条带（1'）; 测试样品引入部分; 以及具有用于在反应部（13）和控制部显示反应结果的显示窗（4）的壳体。 在条带（1'）上。
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