Abstract:
PURPOSE: A medium for maintainig and determining west nile is provided to reduce the exposure by high risk virus and to shorten the use of harmful reagents. CONSTITUTION: A medium for maintaining and detecting west nile contains alpha-MEM or DMEM containing 1-10 g/L of glucose and pH indicator. The pH indicator is phenol red. A potency assay method for detecting west nile comprises: a step of culturing west nile host cells and a sample in the medium; and a step of measuring the pH concentration of the medium to detecting west nile virus and tittering west nile in a sample. The host cells are monkey kidney cells.
Abstract:
PURPOSE: A recombinant adenovirus expressing siRNA for suppressing and preventing foot-and-mouth virus is provided to generate siRNA targeting foot-and-mouth virus 2B and 3C genes and to ensure antiviral effect. CONSTITUTION: A recombinant adenovirus comprises a nucleotide of sequence number 3 or 5 and complementary nucleotide thereof(sequence number 4 or 6). An siRNA which specifically binds to the foot-and-mouth virus is synthesized from a DNA having a nucleotide of sequence number 1 or 2, or complementary nucleotide thereof. An antiviral formulation for foot-and-mouth contains the recombinant adenovirus expressing siRNA as an active ingredient.
Abstract:
본 발명은 구제역바이러스를 구성하는 구조단백질을 코딩하는 유전자와 비구조단백질인 3C 유전자를 적어도 포함하는 재조합베큘로바이러스를 제공한다. 또한, 본 발명은 상기 재조합베큘로바이러스를 숙주세포에 형질전환시켜 구제역바이러스 백신용 항원 및 진단용 항원제시용 재조합단백질을 제조하는 방법을 제공한다. 베큘로바이러스, 백미드, P1, 3C, 항원
Abstract:
본 발명은 구제역바이러스를 구성하는 구조단백질을 코딩하는 유전자와 비구조단백질인 3C 유전자를 적어도 포함하는 재조합베큘로바이러스를 제공한다. 또한, 본 발명은 상기 재조합베큘로바이러스를 숙주세포에 형질전환시켜 구제역바이러스 백신용 항원 및 진단용 항원제시용 재조합단백질을 제조하는 방법을 제공한다. 베큘로바이러스, 백미드, P1, 3C, 항원
Abstract:
PURPOSE: Diagnostic methods of foot-and-mouth disease using recombinant 3ABC non-structural protein expressed in insect cells and a monoclonal antibody is provided, thereby more rapidly and accurately diagnosing the foot-and-mouth disease than the prior methods. CONSTITUTION: A gene encoding a recombinant 3ABC non-structural protein derived from Korean foot-and-mouth disease virus O/SKR/2000 has the nucleotide sequence of SEQ ID NO: 1. A recombinant baculovirus to be expressed in insect cells is prepared by co-transfection with a recombinant vector containing the recombinant 3ABC non-structural protein gene of SEQ ID NO: 1. The recombinant 3ABC non-structural protein is expressed by infection of the insect cells with the recombinant baculovirus. A hybridoma cell line 3F-11(KCTC 10138BP) is prepared by introducing the recombinant 3ABC non-structural protein expressed in E. coli into an animal, collecting an immunized cell from the animal and fusing the immunized cell with a cancer cell. A monoclonal antibody is produced from the hybridoma cell line 3F-11(KCTC 10138BP). A diagnostic method of foot-and-mouth disease comprises the steps of: (1) diluting the recombinant 3ABC non-structural protein in a coating buffer solution and pouring the diluate on a plate; (2) reacting the testing serum with the plate; (3) reacting a conjugate, which binds with an antibody for foot-and-mouth disease virus in the testing serum and has an enzyme, a radioactive material or a fluorescent material, with the testing serum; and (4) measuring intensity of the enzyme reaction, fluorescence reaction or radiation reaction with the conjugate.
Abstract translation:目的:提供使用在昆虫细胞中表达的重组3ABC非结构蛋白和单克隆抗体的口蹄疫诊断方法,从而比现有方法更快速,准确地诊断口蹄疫。 构成:编码来自韩国口蹄疫病毒O / SKR / 2000的重组体3ABC非结构蛋白的基因具有SEQ ID NO:1的核苷酸序列。在昆虫细胞中表达的重组杆状病毒通过 用含有SEQ ID NO:1的重组3ABC非结构蛋白基因的重组载体共转染。重组体3ABC非结构蛋白用重组杆状病毒感染昆虫细胞表达。 通过将在大肠杆菌中表达的重组3ABC非结构蛋白质导入动物中,从动物中收集免疫的细胞并将免疫的细胞与癌细胞融合来制备杂交瘤细胞系3F-11(KCTC 10138BP)。 从杂交瘤细胞系3F-11(KCTC 10138BP)产生单克隆抗体。 口蹄疫的诊断方法包括以下步骤:(1)在包被缓冲溶液中稀释重组体3ABC非结构蛋白,将稀释液倒入平板上; (2)使测试血清与板反应; (3)使与测试血清中的口蹄疫病毒抗体结合并具有酶,放射性物质或荧光材料的缀合物与测试血清反应; 和(4)测量酶反应的强度,荧光反应或与缀合物的辐射反应。