公开(公告)号：SG10201808165YA

公开(公告)日：2018-10-30

申请号：SG10201808165Y

申请日：2014-04-17

Applicant: FLUIDIGM CANADA INC

Inventor： LOBODA ALEXANDRE

Abstract: SAMPLE ANALYSIS FOR MASS CYTOMETRY The invention relates to methods and devices for analysis of samples using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The invention provides methods and devices in which individual ablation plumes are distinctively captured and transferred to the ICP, followed by analysis by mass cytometry. FIG. 1

公开(公告)号：CA2972500A1

公开(公告)日：2016-07-07

申请号：CA2972500

申请日：2015-12-31

Applicant: FLUIDIGM CANADA INC

Inventor： LOBODA ALEXANDER V

IPC: C12M1/34 ,  G01N35/10 ,  H01J49/04

Abstract: Apparatus and methods for delivering biological samples to an ICP source of a mass cytometer are disclosed. Biological material is disposed on a plurality of discrete sites on a carrier. The plurality of discrete sites are configured to retain biological material and to release the biological material upon application of energy. The carrier is positioned in proximity to a gas conduit and upon release from the discrete sites, the biological material becomes entrained in a gas flow, which delivers discrete portions of biological material through the conduit to the ICP source for analysis by mass cytometry. The apparatus and methods can provide a continuous stream of discrete portions of biological material to a mass cytometer.

公开(公告)号：CA2913806A1

公开(公告)日：2014-12-04

申请号：CA2913806

申请日：2014-05-22

Applicant: FLUIDIGM CANADA INC

Inventor： BANDURA DMITRY ,  BARANOV VLADIMIR ,  LOBODA ALEXANDER ,  ORNATSKY OLGA ,  TANNER SCOTT D

IPC: G01N27/62 ,  G01N33/483

Abstract: The invention relates to methods and devices for molecular cytometry. The molecular cytometer is for use in the analysis of molecular tag labeled particles. In the molecular cytometer, volatile molecular tags attached to the particles, such as cells, are released as groups of molecular tags and ionized by the method of soft ionization to produce corresponding groups of molecular ions. The molecular cytometer has two detectors, one to detect the presence of the groups of molecular ions and the other to detect mobility separated molecular ions. The mobility separated molecular ions are synchronized to correspond with the groups of molecular ions.

公开(公告)号：CA2895360A1

公开(公告)日：2014-10-23

申请号：CA2895360

申请日：2014-04-17

Applicant: FLUIDIGM CANADA INC

Inventor： LOBODA ALEXANDRE

IPC: H01J49/04 ,  H01J49/26

Abstract: The invention relates to methods and devices for analysis of samples using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The invention provides methods and devices in which individual ablation plumes are distinctively captured and transferred to the ICP, followed by analysis by mass cytometry.

公开(公告)号：CA2888304A1

公开(公告)日：2014-05-01

申请号：CA2888304

申请日：2013-10-22

Applicant: FLUIDIGM CANADA INC

Inventor： TANNER SCOTT D

IPC: G01N27/00 ,  C12M1/34 ,  C40B20/08 ,  C40B70/00 ,  G01N33/483 ,  G01N33/58 ,  H01J49/40

Abstract: In a mass cytometer system, a tissue sample labeled with multiple metal tags is supported on an encoded substrate for distribution profile mapping by laser ablation. Groups of elemental ions from each plume generated by each laser pulse are detected by the mass cytometer and the data is mapped according to the encoded substrate. This configuration allows for the production of a 3-dimentional distribution profile of the multiple metal tags in the tissue sample.

公开(公告)号：CA2852043A1

公开(公告)日：2013-05-02

申请号：CA2852043

申请日：2012-10-26

Applicant: FLUIDIGM CANADA INC

Inventor： JONG RAYMOND

IPC: G01N15/10 ,  C12M1/34 ,  H01J49/04

Abstract: In a mass cytometer or mass spectrometer, a sample of elemental tagged particles is transferred from a dispersion to a gas flow through a carrier aerosol spray for atomization and ionization by inductively coupled plasma (ICP) source. The configuration of the sample transfer apparatus allow for total consumption of the sample by passing the sample spray through a deceleration stage to decelerate the spray of particles from its high velocity expansion. Following the deceleration stage, the decelerated sample of particles can be accelerated and focused through an acceleration stage for transferring into the ICP. This effectively improves the particle transfer between the sample spray and the ICP.

公开(公告)号：WO2020150236A1

公开(公告)日：2020-07-23

申请号：PCT/US2020/013509

申请日：2020-01-14

Applicant: FLUIDIGM CANADA INC. ,  FLUIDIGM CORPORATION

Inventor： LOBODA, Alexander ,  SANDKUIJL, Daaf ,  CAREW, Adam

IPC: C12Q1/6872 ,  G01N27/62 ,  G01N33/48 ,  H01J49/00

Abstract: As described herein, one or more parameters of a direct ionization imaging mass spectrometer (IMS) may be set to obtain a desired plasma and deliver it to a mass detector. Depending on the application, certain parameters may be predetermined (e.g., a spot size given a desired resolution) and, as described herein, other parameters can be adjusted to obtain the desired plasma properties. Also included is sample preparation suitable for direct ionization IMS and/or other imaging modalities.

公开(公告)号：WO2020092236A1

公开(公告)日：2020-05-07

申请号：PCT/US2019/058342

申请日：2019-10-28

Applicant: FLUIDIGM CANADA INC. ,  FLUIDIGM CORPORATION

Inventor： LOBODA, Alexander ,  CAREW, Adam

IPC: H01J49/04 ,  G01N1/28 ,  G01N15/10 ,  H01J49/10 ,  G01N21/68 ,  G01N21/73 ,  H01J49/105 ,  H05H1/30

Abstract: We describe in this application the analysis of samples using elemental or mass spectrometry and the analysis of samples, such as biological samples by suspension mass cytometry or imaging mass cytometry and an inductively coupled plasma torch with reverse vortex flow for elemental analysis and a method of operating an ICP torch configured to interface with a spectrometer.

公开(公告)号：WO2020055813A1

公开(公告)日：2020-03-19

申请号：PCT/US2019/050333

申请日：2019-09-10

Applicant: FLUIDIGM CANADA INC. ,  FLUIDIGM CORPORATION

Inventor： SANDKUIJL, Daaf ,  LOBODA, Alexander ,  CAREW, Adam ,  LAKSHMAN, Minalini

IPC: H01J49/04 ,  G01N1/28 ,  H01J49/02 ,  H01J49/06 ,  H01J49/26

Abstract: This disclosure relates to systems and methods for high speed modulation sample imaging. Disclosed herein are systems and methods for performing imaging mass cytometry, including analysis of labelling atoms by elemental (e.g., atomic) mass spectrometry. Aspects include a sampling system having, and method of using, a femtosecond (fs) laser and/or laser scanning. Alternatively or in addition, aspects include systems and methods for co-registering other imaging modalities with imaging mass cytometry.

公开(公告)号：WO2020055810A1

公开(公告)日：2020-03-19

申请号：PCT/US2019/050330

申请日：2019-09-10

Applicant: FLUIDIGM CANADA INC. ,  FLUIDIGM CORPORATION

Inventor： SANDKUIJL, Daaf ,  LOBODA, Alexander ,  CAREW, Adam ,  ASKARPOUR, Khashayar

IPC: G01N1/04 ,  G02B21/00 ,  H01J49/04 ,  H01J49/10

Abstract: The invention relates to systems and methods for autofocusing in imaging mass spectrometry. The present invention provides improvements over current IMS and IMG apparatus and methods through an autofocus component including plurality of apertures in the autofocus system, such as a plurality of apertures arranged in 2 dimensions. As a plurality of apertures is used the autofocus system provides redundancy in the event that measurement of focus on the sample from the illuminating radiation passed through one or more of the apertures fails so reducing the number of unsuccessful autofocus attempts.