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公开(公告)号：WO2020198614A1
公开(公告)日：2020-10-01
申请号：PCT/US2020/025296
申请日：2020-03-27
Applicant: FLUIDIGM CANADA INC. , FLUIDIGM CORPORATION
Inventor： LI, Stephen K.H. , MAJONIS, Daniel , BARANOV, Vladimir , ORNATSKY, Olga , ALLO, Bedilu , BANDURA, Dmitry , THOM, Colin , STELZER, Greg , LOBODA, Alexander
Abstract: A lyophilized antibody panel is disclosed for interrogation using elemental analysis. The antibody panel includes multiple antibodies each element-tagged or element-labelled with one or more isotopes such that each different antibody is isotopically distinguishable from the other antibodies. Each element tag can include one or more unique isotopes or unique combinations of isotopes. The set of element-tagged antibodies can be lyophilized in admixture. Thus, the lyophilized element-tagged antibody panel can be easily and efficiently resuspended and mixed with a sample prior to interrogation with an elemental analyzer, such as a mass spectrometer. This lyophilized element-tagged antibody panel can provide the benefits of an element-tagged assay while also being easy to use and remaining stable for long durations.
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公开(公告)号：WO2020257258A1
公开(公告)日：2020-12-24
申请号：PCT/US2020/038097
申请日：2020-06-17
Applicant: FLUIDIGM CORPORATION , FLUIDIGM CANADA INC.
Inventor： CORKUM, Paul , LOBODA, Alexander
Abstract: Embodiments of the present invention relate to replacement of the previous ICP-based ionisation system with a new laser ionisation system, providing improved mass spectrometer-based apparatus and methods for using them to analyse samples, in particular the use of mass spectrometry mass cytometry, imaging mass spectrometry and imaging mass cytometry, for the analysis of biological samples. Accordingly, embodiments of the present invention provide an apparatus, for example a mass cytometer, comprising: 1 ) a sampler; 2) a laser ionisation system to receive material removed from the sample by the sampler, wherein the laser ionisation system comprises an ionisation system conduit and a pulsed laser adapted to ionise sample material passing through or exiting the ionisation system conduit; and 3) a mass spectrometer to receive elemental ions from said ionisation system and to analyse said elemental ions.
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公开(公告)号：WO2019246033A1
公开(公告)日：2019-12-26
申请号：PCT/US2019/037644
申请日：2019-06-18
Applicant: FLUIDIGM CANADA INC. , FLUIDIGM CORPORATION , CORKUM, Paul
Inventor： CORKUM, Paul , LOBODA, Alexander
Abstract: The present invention relates to the high resolution imaging of samples using imaging mass spectrometry (IMS) and to the imaging of biological samples by imaging mass cytometry (IMCTM) in which labelling atoms are detected by IMS. LA-ICP-MS (a form of IMS in which the sample is ablated by a laser, the ablated material is then ionised in an inductively coupled plasma before the ions are detected by mass spectrometry) has been used for analysis of various substances, such as mineral analysis of geological samples, analysis of archaeological samples, and imaging of biological substances. However, traditional LA-ICP-MS systems and methods may not provide high resolution. Described herein are methods and systems for high resolution IMS and IMC.
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公开(公告)号：WO2020150236A1
公开(公告)日：2020-07-23
申请号：PCT/US2020/013509
申请日：2020-01-14
Applicant: FLUIDIGM CANADA INC. , FLUIDIGM CORPORATION
Inventor： LOBODA, Alexander , SANDKUIJL, Daaf , CAREW, Adam
IPC: C12Q1/6872 , G01N27/62 , G01N33/48 , H01J49/00
Abstract: As described herein, one or more parameters of a direct ionization imaging mass spectrometer (IMS) may be set to obtain a desired plasma and deliver it to a mass detector. Depending on the application, certain parameters may be predetermined (e.g., a spot size given a desired resolution) and, as described herein, other parameters can be adjusted to obtain the desired plasma properties. Also included is sample preparation suitable for direct ionization IMS and/or other imaging modalities.
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公开(公告)号：WO2020092236A1
公开(公告)日：2020-05-07
申请号：PCT/US2019/058342
申请日：2019-10-28
Applicant: FLUIDIGM CANADA INC. , FLUIDIGM CORPORATION
Inventor： LOBODA, Alexander , CAREW, Adam
Abstract: We describe in this application the analysis of samples using elemental or mass spectrometry and the analysis of samples, such as biological samples by suspension mass cytometry or imaging mass cytometry and an inductively coupled plasma torch with reverse vortex flow for elemental analysis and a method of operating an ICP torch configured to interface with a spectrometer.
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Patent Agency Ranking
公开(公告)号：WO2020055813A1
公开(公告)日：2020-03-19
申请号：PCT/US2019/050333
申请日：2019-09-10
Applicant: FLUIDIGM CANADA INC. , FLUIDIGM CORPORATION
Inventor： SANDKUIJL, Daaf , LOBODA, Alexander , CAREW, Adam , LAKSHMAN, Minalini
Abstract: This disclosure relates to systems and methods for high speed modulation sample imaging. Disclosed herein are systems and methods for performing imaging mass cytometry, including analysis of labelling atoms by elemental (e.g., atomic) mass spectrometry. Aspects include a sampling system having, and method of using, a femtosecond (fs) laser and/or laser scanning. Alternatively or in addition, aspects include systems and methods for co-registering other imaging modalities with imaging mass cytometry.
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公开(公告)号：WO2020055810A1
公开(公告)日：2020-03-19
申请号：PCT/US2019/050330
申请日：2019-09-10
Applicant: FLUIDIGM CANADA INC. , FLUIDIGM CORPORATION
Inventor： SANDKUIJL, Daaf , LOBODA, Alexander , CAREW, Adam , ASKARPOUR, Khashayar
Abstract: The invention relates to systems and methods for autofocusing in imaging mass spectrometry. The present invention provides improvements over current IMS and IMG apparatus and methods through an autofocus component including plurality of apertures in the autofocus system, such as a plurality of apertures arranged in 2 dimensions. As a plurality of apertures is used the autofocus system provides redundancy in the event that measurement of focus on the sample from the illuminating radiation passed through one or more of the apertures fails so reducing the number of unsuccessful autofocus attempts.
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公开(公告)号：WO2019200307A1
公开(公告)日：2019-10-17
申请号：PCT/US2019/027306
申请日：2019-04-12
Applicant: FLUIDIGM CANADA INC. , FLUIDIGM CORPORATION
Inventor： BARANOV, Vladimir , LOBODA, Alexander , SULLIVAN, Michael
IPC: G01N33/50 , G01N33/532 , G01N33/543 , G01N33/544 , G01N33/566 , G01N33/58 , G01N30/72
Abstract: Analyzing samples injected into an inductively coupled plasma source can be improved by one or more of a stabilizing solution mixable with a sample prior to injection and a heated injector. The stabilizing solution can minimize the difference in osmotic pressure between the solution and the cells with a relatively low amount of dissolved solids (e.g., at or below about 0.2%). The stabilizing solution can contain a salt (e.g., ammonium nitrate) present in concentrations of at least 5 mM. The injector can be heated before and/or during injection. In some cases, heat from adjacent parts can be channeled into the injector to improve heating of the injector. An injector heated to sufficient temperatures can minimize solute buildup and can extend the usable time between cleanings. These improvements can be especially useful in elemental analysis, such as inductively coupled plasma mass spectrometry or inductively coupled plasma optical emission spectrometry.
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公开(公告)号：WO2022060624A1
公开(公告)日：2022-03-24
申请号：PCT/US2021/049667
申请日：2021-09-09
Applicant: FLUIDIGM CANADA INC.
Inventor： LOBODA, Alexander , JONG, Raymond , SULLIVAN, Michael , VOROBIEV, Serguei , ROTENBERG, Robert , STRATULATIV, Emil , VORONOV, Maxim , ARMSTRONG, Mark
Abstract: Inductively coupled plasma (ICP) analyzers use an ICP torch to generate a plasma in which a sample is atomized an ionized. Analysis of the atomic ions can be performed by atomic analysis, such as mass spectrometry (MS) or atomic emission spectrometry (AES). Particle based ICP analysis includes analysis of particles such as cells, beads, or laser ablation plumes, by atomizing and ionizing particles in an ICP torch followed by atomic analysis. In mass cytometry, mass tags of particles are analyzed by mass spectrometry, such as by ICP-MS. Systems and methods of the subject application include one or more of: a demountable ICP torch holder assembly, an external ignition device; an ICP load coil comprising an annular fin, particle suspension sample introduction fluidics, and ICP analyzers thereof.
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公开(公告)号：WO2014190429A1
公开(公告)日：2014-12-04
申请号：PCT/CA2014/050474
申请日：2014-05-22
Applicant: FLUIDIGM CANADA INC.
Inventor： BANDURA, Dmitry , BARANOV, Vladimir , LOBODA, Alexander , ORNATSKY, Olga , TANNER, Scott
IPC: G01N27/62 , G01N33/483
CPC classification number: G01N33/54366 , G01N15/1031 , G01N15/1404 , G01N15/1459 , G01N27/622 , G01N27/624 , G01N33/483 , G01N33/532 , G01N33/54313 , G01N2015/1006 , G01N2458/15
Abstract: The invention relates to methods and devices for molecular cytometry. The molecular cytometer is for use in the analysis of molecular tag labeled particles. In the molecular cytometer, volatile molecular tags attached to the particles, such as cells, are released as groups of molecular tags and ionized by the method of soft ionization to produce corresponding groups of molecular ions. The molecular cytometer has two detectors, one to detect the presence of the groups of molecular ions and the other to detect mobility separated molecular ions. The mobility separated molecular ions are synchronized to correspond with the groups of molecular ions.
Abstract translation: 本发明涉及用于分子细胞计数的方法和装置。 分子光谱仪用于分子标签标记颗粒的分析。 在分子细胞仪中，附着于粒子（如细胞）的挥发性分子标记作为分子标签的基团释放，并通过软电离方法离子化以产生相应的分子离子组。 分子细胞仪有两个检测器，一个用于检测分子离子组的存在，另一个用于检测迁移率分离的分子离子。 迁移率分离的分子离子被同步以对应于分子离子的组。
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