METHOD FOR PRODUCING CELLULASE AND APPARATUS FOR SAID METHOD

    公开(公告)号:IN3792CHN2014A

    公开(公告)日:2015-10-16

    申请号:IN3792CHN2014

    申请日:2014-05-20

    Abstract: A method for producing a cellulase comprising steps (1) to (3) mentioned below wherein the resultant cellulase can be used effectively in washing agents and for the hydrolysis of starches the hydrolysis of celluloses and so on: (1) filtrating an aqueous solution of a cellulase originated from a filamentous fungus through an ultrafiltration membrane having a cut off molecular weight of 100 000 to 200 000 to produce a filtrate and a concentrated enzyme solution that is a non filtrate; (2) filtering the filtrate produced in step (1) through a second ultrafiltration membrane having a cut off molecular weight of 5 000 to 50 000 to produce a second concentrated enzyme solution that is a non filtrate; and (3) mixing the concentrated enzyme solutions produced in steps (1) and (2) together to produce a cellulase originated from the filamentous fungus.

    Procedimiento para producir glucosidasa, composición enzimática y procedimiento de hidrólisis de biomasa

    公开(公告)号:ES2544710T3

    公开(公告)日:2015-09-03

    申请号:ES11750425

    申请日:2011-01-26

    Abstract: Procedimiento para producir una β-glucosidasa mutante derivada de un termófilo que tiene fijada selectivamente a ella una cadena de azúcares y también tiene una actividad glucosidasa, que comprende: (i) preparar ADN que codifica una β-glucosidasa mutante derivada de un termófilo introduciendo una secuencia de ADN que codifica Asn-X-Ser o Asn-X-Thr (en las que, X es cualquier aminoácido excepto prolina) en ADN que codifica una β-glucosidasa derivada de un termófilo que está originalmente desprovista de una secuencia de glucosilación y, además, añadiendo una secuencia de ADN que codifica una secuencia señal de secreción al ADN que codifica una β-glucosidasa mutante, (ii) introducir el ADN que codifica una β-glucosidasa mutante al que se le ha añadido la secuencia de ADN que codifica la secuencia señal de secreción en un microorganismo eucariota, de modo que una β-glucosidasa mutante codificada por el ADN de la β-glucosidasa mutante se exprese como una proteína secretora, y (iii) aislar y purificar la β-glucosidasa mutante expresada de este modo como una proteína secretora.

    METHOD FOR PRODUCING LACTIC ACID AND METHOD FOR PRODUCING POLYLACTIC ACID

    公开(公告)号:SG2013095930A

    公开(公告)日:2015-07-30

    申请号:SG2013095930

    申请日:2009-12-25

    Abstract: Lactic acid obtained by a method for producing lactic acid, the method comprising: (A) a step of continuous fermentation wherein a fermentation culture medium of a microorganism having an ability of lactic acid fermentation is filtered through a porous membrane having an average pore size of not less than 0.01 µm and less than 1 µm with a transmembrane pressure difference within the range of 0.1 to 20 kPa, and the permeate is collected, while retaining the non-permeated liquid in or returning the non-permeated liquid to the culture, and adding a fermentation feedstock to the culture; (B) a step of filtering the permeate obtained in Step (A) through a nanofiltration membrane; and (C) a step of distilling the permeate obtained in Step (B) under a pressure of not less than 1 Pa and not more than atmospheric pressure, at 25°C to 200°C to recover lactic acid; comprises only small amounts of impurities, and a polylactic acid obtained using the lactic acid as a raw material has excellent thermal stability, mechanical strength and hue.

    Method for producing chemical and continuous culture system

    公开(公告)号:AU2009299152B2

    公开(公告)日:2015-07-16

    申请号:AU2009299152

    申请日:2009-09-16

    Abstract: To control the flow rate of a liquid culture medium in a membrane separation tank without affecting the culture conditions in a culture tank and to increase the production efficiency of a chemical by inhibiting the precipitation of a microorganism or culture cells, provided is a method for producing a chemical which comprises culturing a microorganism or culture cells in a culture tank, feeding the liquid culture medium from the culture tank to a membrane separation tank and filtering the same through a separation membrane, collecting a fermentation product as the chemical from the permeate and, at the same time, returning the unfiltered liquid culture medium not having been filtered in such a manner as combining the same with the liquid culture medium in the upstream side of the membrane separation tank, wherein a portion of the liquid culture medium fed from the culture tank is passed outside the membrane separation tank depending on the inflow side pressure of the membrane separation tank.

    Butanol manufacturing method
    107.
    发明专利

    公开(公告)号:AU2011228079B2

    公开(公告)日:2015-06-25

    申请号:AU2011228079

    申请日:2011-03-16

    Abstract: High purity butanol can be manufactured by means of the disclosed butanol manufacturing method which comprises: a manufacturing step (A) wherein a butanol-containing solution is filtered by being passed through a nano-filtration membrane, and the butanol-containing solution is collected from the permeation side; a manufacturing step (B) wherein the butanol-containing solution obtained in step (A) is concentrated by being passed through a reverse osmosis membrane, and separated into two phases, namely a butanol phase and a water phase; and a manufacturing step (C) wherein butanol is collected from the butanol phase obtained in step (B).

    METHOD OF PRODUCING A SUGAR LIQUID
    108.
    发明专利

    公开(公告)号:CA2921891A1

    公开(公告)日:2015-02-26

    申请号:CA2921891

    申请日:2014-08-21

    Abstract: The present invention relates to a method for manufacturing a sugar solution comprising the following steps (1) to (3): step (1), a step for pretreating a first cellulose-containing biomass; step (2), a step for adding a second cellulose-containing biomass in an amount in the range of 1 to 50% with respect to the solid weight of the first cellulose-containing biomass pretreated substance of step (1), and performing hydrolyzation using a mold-derived cellulase; and step (3), a step for subjecting the hydrolysate to solid-liquid separation to obtain a solution component and a hydrolysis residue, and filtering the solution component through an ultrafiltration membrane to recover the mold-derived cellulase as a non-permeate and recover the sugar solution as a permeate.

    Process for producing sugar solution

    公开(公告)号:AU2013272652A1

    公开(公告)日:2014-12-04

    申请号:AU2013272652

    申请日:2013-06-04

    Abstract: The present invention provides a process for producing a sugar solution having improved fermentability from cellulosic biomass as a raw material. This process for producing a sugar solution comprises: a step in which an alkali is added to a cellulosic-biomass-derived concentrated sugar solution to regulate the pH thereof to 7 or higher, thereby precipitating insoluble substances containing at least magnesium; and a step in which the resulting mixture is filtered with a microfiltration membrane to remove the insoluble substances and obtain a sugar solution as the penetrant.

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