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公开(公告)号：KR101442682B1
公开(公告)日：2014-09-19
申请号：KR1020120038484
申请日：2012-04-13
Applicant: 서강대학교산학협력단
Abstract: 본 발명은 (a) 미생물의 바이오매스를 냉장 메탄올(cold methanol)에 현탁한 다음 동결시키는 단계; (b) 상기 동결된 바이오매스를 해동시키는 단계; (c) 상기 단계 (a) 및 (b)를 반복하여 상기 바이오매스로부터 누출된 대사물의 현탁액을 얻는 단계; 및 (d) 상기 대사물의 현탁액에 대하여 매스 스펙트로미트리(mass spectrometry) 분석을 실시하여 상기 미생물의 세포 내 대사물을 동정하는 단계를 포함하는 미생물의 세포 내 대사 프로파일(intracellular metabolic profile)을 얻는 방법을 제공한다. 본 발명의 방법에 따르면, 본 발명은 고생산 및 고수율의 대사 프로파일을 제공할 수 있다.
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公开(公告)号：KR1020140071293A
公开(公告)日：2014-06-11
申请号：KR1020140046079
申请日：2014-04-17
Applicant: 서강대학교산학협력단
Abstract: The present invention relates to transformed yeast for improving the content of fatty acids and a preparation method thereof and provides the transformed yeast for producing fatty acids which is transformed with a recombination vector comprising; (i) a nucleotide sequence which codes an oxoacyl carrier protein synthetase II (fabF) derived from Saccharomyces cerevisiae; (ii) a nucleotide sequence which codes oxoacyl carrier protein reductase (fabG) derived from Streptococcus pyogenes; (iii) a nucleotide sequence which codes a hydroxyl myristoyl carrier protein dehydratase (fabZ) derived from Streptococci pyogenes; (iv) a nucleotide sequence which codes ynoyl carrier protein reductase II (fabI) derived from Streptococcus pyogenes; or (v) a nucleotide sequence which codes oleyl carrier protein hydrolase (EC 3.1.2.14) derived from Streptococcus pyogenes. The present invention increases the content of the fatty acids itself by over-expressing an extension step of a fatty acid biosynthesis path by Saccharomyces cerevisiae and mass-produces fatty acid biosynthesis through a metabolism flow change due to the over-expression of the acid biosynthesis path from glucose and the insertion of other species.
Abstract translation: 本发明涉及用于改善脂肪酸含量的转化酵母及其制备方法，并提供用于制备用重组载体转化的脂肪酸的转化酵母，其包含： （i）编码源自酿酒酵母的氧代酰基载体蛋白质合成酶II（fabF）的核苷酸序列; （ii）编码源自化脓性链球菌的氧代酰基载体蛋白还原酶（fabG）的核苷酸序列; （iii）编码源自链球菌化脓链球菌的羟基肉豆蔻酰载体蛋白脱水酶（fabZ）的核苷酸序列; （iv）编码源自化脓性链球菌的月桂酰载体蛋白还原酶II（fabI）的核苷酸序列; 或（v）编码源自化脓链球菌的油基载体蛋白水解酶（EC 3.1.2.14）的核苷酸序列。 本发明通过过表达酿酒酵母的脂肪酸生物合成途径的延伸步骤而增加脂肪酸本身的含量，并且由于酸生物合成途径的过度表达而通过代谢流动变化大规模生产脂肪酸生物合成 从葡萄糖和其他物种的插入。
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Patent Agency Ranking
公开(公告)号：KR1020130115815A
公开(公告)日：2013-10-22
申请号：KR1020120038484
申请日：2012-04-13
Applicant: 서강대학교산학협력단
Abstract: PURPOSE: An extraction and analysis method of microorganisms for acquiring an intracellular metabolic profile is provided to obtain a metabolic profile of high productivity and high yield and to construct an optimal method for preparing intracellular metabolites. CONSTITUTION: A method for acquiring an intracellular metabolic profile in microorganisms comprises the steps of: suspending microorganism biomass in cold methanol and freezing the biomass; thawing the biomass and repeating the steps; collecting a suspension of the metabolites released from the biomass; and performing mass spectrometry of the suspension and identifying the intracellular metabolites.
Abstract translation: 目的：提供获取细胞内代谢特征的微生物提取和分析方法，以获得高生产率和高产量的代谢特征，并构建细胞内代谢产物的最佳方法。 构成：在微生物中获得细胞内代谢分布的方法包括以下步骤：将微生物生物质悬浮在冷甲醇中并冷冻生物质; 解冻生物质并重复步骤; 收集从生物质释放的代谢物的悬浮液; 并进行悬浮液的质谱分析和鉴定细胞内代谢物。
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公开(公告)号：KR1020130058228A
公开(公告)日：2013-06-04
申请号：KR1020110124125
申请日：2011-11-25
Applicant: 서강대학교산학협력단
Abstract: PURPOSE: A method for preparing fatty acid-overexpressing transformed E.coli is provided to enhance conversion of a precursor of fatty acid biosynthesis pathway and to produce a large amount of the fatty acid. CONSTITUTION: A method for preparing fatty acid-overexpressing transformed E.coli comprises: a step of inserting one or more nucleotide sequenced selected from a group consisting of nucleotide sequences encoding fabF(3-oxoacyl-[ACP] synthase II), fabG(3-oxoacyl-[ACP] reductase), fabZ(3R-hydroxymyristol acyl carrier protein dehydratase), and fabI(enoyl-[ACP] reductase) into a vector and preparing a recombinant vector; and a step of transforming the recombinant vector into E.coli. A method for biosynthesis of fatty acids comprises a step of culturing the transformed E.coli.
Abstract translation: 目的：提供一种制备脂肪酸过表达转化大肠杆菌的方法，以增强脂肪酸生物合成途径前体的转化并产生大量的脂肪酸。 构成：制备脂肪酸过表达转化大肠杆菌的方法包括：插入一个或多个选自编码fabF（3-氧代酰基 - [ACP]合酶II）的核苷酸序列，fabG（3） - 氧酰基 - [ACP]还原酶），fabZ（3R-羟基肉豆蔻酰基载体蛋白脱水酶）和fabI（烯酰基 - [ACP]还原酶）），并制备重组载体; 以及将重组载体转化到大肠杆菌中的步骤。 脂肪酸的生物合成方法包括培养转化的大肠杆菌的步骤。
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公开(公告)号：KR1020120128776A
公开(公告)日：2012-11-28
申请号：KR1020110046626
申请日：2011-05-18
Applicant: 서강대학교산학협력단
Abstract: PURPOSE: A Klebsiella pneumoniae strain for overexpression of 2,3-butandiol is provided to enhance 2,3-butandiol production. CONSTITUTION: A Klebsiella pneumoniae strain for overexpression of 2,3-butandiol is prepared by transformation or cotransformation with an expression vector containing a nucleotide sequence. The expression vector contains: a nucleotide encoding acetolactate decarboxylase; a nucleotide sequence encoding acetolactate synthase; and a nucleotide sequence encoding acetoin reductase.
Abstract translation: 目的：提供用于过度表达2,3-丁二醇的肺炎克雷伯杆菌菌株以增强2,3-丁二醇生产。 构成：通过用含有核苷酸序列的表达载体进行转化或共转化制备用于过表达2,3-丁二醇的肺炎克雷伯杆菌菌株。 表达载体含有：编码乙酰乳酸脱羧酶的核苷酸; 编码乙酰乳酸合酶的核苷酸序列; 和编码乙偶姻还原酶的核苷酸序列。
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116.发明公开2, 3-부탄다이올 생산량 향상을 위한 락테이트 디하이드로겐에이즈가 제거 된 돌연변이 엔테로박터 에어로진스 无效LACTATE DEHYDROGENASE去除了用于增强2,3-丁二醇生产的肠内营养素
公开(公告)号：KR1020120104765A
公开(公告)日：2012-09-24
申请号：KR1020110022372
申请日：2011-03-14
Applicant: 서강대학교산학협력단 , 고려대학교 산학협력단
Abstract: PURPOSE: A lactate dehydrogenase gene-deleted Enterobacter aerogenes is provided to highly prepare 2,3-butandiol and to be used as alternative energy. CONSTITUTION: A lactate dehydrogenase gene-deleted Enterobacter aerogenes has improved 2,3-butandiol productivity. A vector for removing the gene contains flanking sequence of lactate dehydrogenase gene and FRT-kanamyucin-FRT cassette. The vector is applied to the Enterobacter aerogenes. A method for preparing the Enterobacter aerogenes comprises: a step of transforming the vector to Enterobacter aerogenes to remove the lactate dehydrogenase gene by electroporation; and a step of selecting lactate dehydrogenase gene-deleted Enterobacter aerogenes.
Abstract translation: 目的：提供乳酸脱氢酶基因缺失的产气肠杆菌高度制备2,3-丁二醇并用作替代能量。 构成：乳酸脱氢酶基因缺失的产气肠杆菌具有改善的2,3-丁二醇生产力。 用于除去该基因的载体含有乳酸脱氢酶基因和FRT-卡纳米霉素-FRT盒的侧翼序列。 将载体应用于产气肠杆菌。 制备产气肠杆菌的方法包括：将载体转化成产气肠杆菌以通过电穿孔除去乳酸脱氢酶基因的步骤; 以及选择乳酸脱氢酶基因缺失的产气肠杆菌的步骤。
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公开(公告)号：KR1020110088777A
公开(公告)日：2011-08-04
申请号：KR1020100008443
申请日：2010-01-29
Applicant: 서강대학교산학협력단 , 한국과학기술원
Abstract: PURPOSE: A method for culturing a strain which produces succinic acid is provided to obtain a large amount of succinic acid and to be used for a novel microorganism fermentation. CONSTITUTION: A method for determining amino acid composition of a culture medium for a strain which produces succinic acid comprises: a step of culturing the strain in a medium containing one or more amino acids among 20 kinds of amino acids; and a step of quantitating the concentration of NAD, NADH, NADP, or NADPH in the strain.
Abstract translation: 目的：提供一种培养产生琥珀酸的菌株的方法，以获得大量的琥珀酸并用于新的微生物发酵。 构成：用于测定产生琥珀酸的菌株的培养基的氨基酸组成的方法，包括：在20种氨基酸中含有一个或多个氨基酸的培养基中培养菌株的步骤; 以及定量菌株中NAD，NADH，NADP或NADPH浓度的步骤。
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