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    SYSTEMS, METHODS AND COMPOSITIONS FOR SIMULTANEOUS DETECTION OF RNA AND PROTEIN BY MASS SPECTROMETRY
    125.
    发明专利
    SYSTEMS, METHODS AND COMPOSITIONS FOR SIMULTANEOUS DETECTION OF RNA AND PROTEIN BY MASS SPECTROMETRY 未知

    公开(公告)号：CA3029183A1

    公开(公告)日：2017-01-05

    申请号：CA3029183

    申请日：2016-06-29

    Applicant: FLUIDIGM CANADA INC

    Inventor： ORNATSKY OLGA

    IPC: C07H19/00 ,  C07H21/00 ,  C07H21/02

    Abstract: In certain embodiments, a method of detection includes providing cells labeled with a first element tag bound to a target RNA sequence and a second element tag bound to a target protein. The method may further include ionizing a cell, or a portion thereof, to produce an ionized first and second element tag, and simultaneously detecting the ionized first and second element tag. The ionization may be performed by an inductively coupled plasma (ICP) torch. The method may further comprise nebulizing the cells in suspension prior to ionizing. In certain embodiments, the ionized first and second element tag may be detected by time-of-flight mass spectrometry. Additional methods, and systems and reagents for simultaneous detection of RNA and protein by mass spectrometry are also described.

    Marcadores elementales de cadena principal polimérica
    127.
    发明专利
    Marcadores elementales de cadena principal polimérica 未知

    公开(公告)号：ES2553141T3

    公开(公告)日：2015-12-04

    申请号：ES11008035

    申请日：2007-05-28

    Applicant: FLUIDIGM CANADA INC

    Inventor： WINNIK MITCHELL ,  NITZ MARK ,  BARANOV VLADIMIR ,  LOU XUDONG

    IPC: G01N33/53 ,  C08F8/42 ,  C08F220/36

    Abstract: Un método para el análisis de un analito, que comprende: (i) incubar un analito con un marcador elemental o un reactivo de afinidad con marcaje elemental, en donde el marcador elemental o el reactivo de afinidad se une con el analito, en donde, el marcador elemental comprende un polímero, o el reactivo de afinidad con marcaje elemental es marcado con un marcador elemental que comprende un polímero, en donde, el polímero comprende al menos un grupo colgante de unión a metal, que comprende al menos un átomo de metal o es capaz de unirse a al menos un átomo de metal; (ii) separar el reactivo de afinidad marcado no unido del reactivo de afinidad marcado unido o elementos de marcador no unidos de los elementos de marcador unidos; y (iii) analizar el elemento unido al reactivo de afinidad unido al analito o elementos de marcador unidos mediante análisis elemental.

    SAMPLE ANALYSIS BY MASS CYTOMETRY
    129.
    发明专利
    SAMPLE ANALYSIS BY MASS CYTOMETRY 未知

    公开(公告)号：SG11201503036SA

    公开(公告)日：2015-05-28

    申请号：SG11201503036S

    申请日：2013-10-22

    Applicant: FLUIDIGM CANADA INC

    Inventor： TANNER SCOTT

    IPC: C12M1/34 ,  G01N27/00 ,  C40B20/08 ,  C40B70/00 ,  G01N33/483 ,  G01N33/58 ,  H01J49/40

    Abstract: In a mass cytometer system, a tissue sample labeled with multiple metal tags is supported on an encoded substrate for distribution profile mapping by laser ablation. Groups of elemental ions from each plume generated by each laser pulse are detected by the mass cytometer and the data is mapped according to the encoded substrate. This configuration allows for the production of a 3-dimentional distribution profile of the multiple metal tags in the tissue sample.

    IMPROVED MASS CYTOMETRY
    130.
    发明专利
    IMPROVED MASS CYTOMETRY 未知

    公开(公告)号：CA3140663A1

    公开(公告)日：2020-12-24

    申请号：CA3140663

    申请日：2020-06-17

    Applicant: FLUIDIGM CANADA INC

    Inventor： CORKUM PAUL ,  LOBODA ALEXANDER ,  RAYNER DAVID M

    IPC: G01N27/62 ,  G01N15/10 ,  G01N21/71 ,  H01J49/04 ,  H01J49/26 ,  H01J49/40

    Abstract: Embodiments of the present invention relate to replacement of the previous ICP-based ionisation system with a new laser ionisation system, providing improved mass spectrometer-based apparatus and methods for using them to analyse samples, in particular the use of mass spectrometry mass cytometry, imaging mass spectrometry and imaging mass cytometry, for the analysis of biological samples. Accordingly, embodiments of the present invention provide an apparatus, for example a mass cytometer, comprising: 1 ) a sampler; 2) a laser ionisation system to receive material removed from the sample by the sampler, wherein the laser ionisation system comprises an ionisation system conduit and a pulsed laser adapted to ionise sample material passing through or exiting the ionisation system conduit; and 3) a mass spectrometer to receive elemental ions from said ionisation system and to analyse said elemental ions.

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