公开(公告)号：AU2011323141B2

公开(公告)日：2016-04-07

申请号：AU2011323141

申请日：2011-11-04

Applicant: ACADEMIA SINICA

Inventor： WONG CHI-HUEY ,  MA CHE ,  TSENG YUNG-CHIEH

IPC: C12P21/06 ,  C12N15/09

Abstract: Methods for production of virus particles with simplified glycosylation on structural or surface proteins are provided. When used as targets for vaccine production, the conserved nature of such sites generates vaccines that are less sensitive to viral mutations. Use of glycosylation inhibitors for production of viruses with simplified glycosylation profiles are disclosed. An exemplary disclosure of influenza viruses and methods for production of mono-glycosylated influenza virus particles is provided. Methods for production of mono-glycosylated forms of influenza A virus, NIBRG-14 (H5N1) are provided.

公开(公告)号：CA2959030A1

公开(公告)日：2016-02-25

申请号：CA2959030

申请日：2015-08-21

Applicant: ACADEMIA SINICA

Inventor： WONG CHI-HUEY ,  WU CHUNG-YI ,  LEE HSIN-YU

IPC: C07K14/33 ,  C07K14/34

Abstract: This disclosure includes an immunogenic composition containing (a) a glycan conjugate including a carrier and one or more glycans, wherein each of the one or more glycans is conjugated with the carrier through a linker, and optionally (b) an adjuvant. The one or more glycan is each a Globo H derivative.

公开(公告)号：HK1207668A1

公开(公告)日：2016-02-05

申请号：HK15108043

申请日：2015-08-19

Applicant: ACADEMIA SINICA

Inventor： WONG CHI-HUEY ,  WU CHUNG-YI ,  TSAI TSUNG-I

IPC: C12P20060101 ,  C12N20060101

Abstract: A novel UDP-Gal regeneration process and its combined use with a galactosyltransferase to add galactose to a suitable acceptor substrate. Also described herein are synthetic methods for generating Globo-series oligosaccharides in large scale, wherein the methods may involve the combination of a glycosyltransferase reaction and a nucleotide sugar regeneration process.

公开(公告)号：CA2950440A1

公开(公告)日：2015-12-03

申请号：CA2950440

申请日：2015-05-27

Applicant: ACADEMIA SINICA

Inventor： WONG CHI-HUEY ,  WU CHUNG-YI

IPC: C07K16/00

Abstract: The present disclosure relates to a novel class of anti-HER2 monoclonal antibodies comprising a homogeneous population of anti-HER2 IgG molecules having the same N-glycan on each of Fc. The antibodies of the invention can be produced from anti-HER2 monoclonal antibodies by Fc glycoengineering. Importantly, the antibodies of the invention have improved therapeutic values with increased ADCC activity and increased Fc receptor binding affinity compared to the corresponding monoclonal antibodies that have not been glycoengineered.

公开(公告)号：AU2013305827A1

公开(公告)日：2015-03-05

申请号：AU2013305827

申请日：2013-08-21

Applicant: SHIE JIUN-JIE ,  ACADEMIA SINICA ,  WONG CHI-HUEY ,  FANG JIM-MIN

Inventor： WONG CHI-HUEY ,  FANG JIM-MIN ,  SHIE JIUN-JIE

IPC: C07D311/06 ,  A61K31/37 ,  A61P29/00 ,  C07D491/04 ,  C07D493/04

Abstract: Provided are benzocyclooctyne compounds of formula (I). These compounds undergo strain-promoted azide-alkyne cyclo additions (SPAAC) without presence of toxic metal catalysts. The provided compounds are useful for diagnosis and imaging of azide-containing molecules. Methods for detection and imaging biomolecules using compounds of the present disclosure are disclosed.

公开(公告)号：CL2014002415A1

公开(公告)日：2015-01-02

申请号：CL2014002415

申请日：2014-09-12

Applicant: ACADEMIA SINICA

Inventor： WONG CHI-HUEY ,  MA CHE ,  WANG CHENG-CHI ,  CHEN JUINE-RUEY

IPC: A61K39/12 ,  A61K39/145 ,  A61K39/155 ,  A61K39/21 ,  A61K39/29 ,  C07K14/135 ,  C07K14/155 ,  C07K14/18

公开(公告)号：CA2882294A1

公开(公告)日：2014-02-27

申请号：CA2882294

申请日：2013-08-20

Applicant: ACADEMIA SINICA

Inventor： WONG CHI-HUEY ,  WU CHUNG-YI ,  TSAI TSUNG-I

IPC: C12P19/04 ,  C12N9/10

Abstract: A novel UDP-Gal regeneration process and its combined use with a galactosyltransferease to add galactose to a suitable acceptor substrate. Also described herein are synthetic methods for generating Globo-series oligosaccharides in large scale, wherein the methods may involve the combination of a glycosyltransferase reaction and a nucleotide sugar regeneration process.

公开(公告)号：DK2318832T3

公开(公告)日：2014-01-20

申请号：DK09798721

申请日：2009-07-15

Applicant: ACADEMIA SINICA

Inventor： WU CHUNG-YI ,  TSENG SUSAN Y ,  WONG CHI-HUEY

IPC: C40B40/12 ,  C40B50/14

公开(公告)号：AU2011323141A1

公开(公告)日：2013-06-27

申请号：AU2011323141

申请日：2011-11-04

Applicant: ACADEMIA SINICA

Inventor： WONG CHI-HUEY ,  MA CHE ,  TSENG YUNG-CHIEH

IPC: C12P21/06 ,  C12N15/09

Abstract: Methods for production of virus particles with simplified glycosylation on structural or surface proteins are provided. When used as targets for vaccine production, the conserved nature of such sites generates vaccines that are less sensitive to viral mutations. Use of glycosylation inhibitors for production of viruses with simplified glycosylation profiles are disclosed. An exemplary disclosure of influenza viruses and methods for production of mono-glycosylated influenza virus particles is provided. Methods for production of mono-glycosylated forms of influenza A virus, NIBRG-14 (H5N1) are provided.

公开(公告)号：MX2010013932A

公开(公告)日：2013-03-01

申请号：MX2010013932

申请日：2009-08-06

Applicant: ACADEMIA SINICA

Inventor： WONG CHI-HUEY ,  WU CHUNG-YI ,  YU JOHN ,  YU ALICE

IPC: A61K39/00 ,  A61K39/385

Abstract: Se proporcionan composiciones inmunogénicas, vacunas de cáncer y métodos para tratar cáncer. Se proporcionan composiciones que comprenden: (a) un glicano tal como Globo H o un fragmento inmunogénico del mismo, en donde el glicano está conjugado con una proteína portadora mediante un enlazador tal como para-nitrofenilo; y (b) un adyuvante que comprende glucolípido capaz de enlazar CDId sobre una célula dendrítica, tal como un derivado de a-galactosilceramida, en donde la composición inmunogénica induce una respuesta inmune que induce un nivel relativo más alto de anticuerpos de isotipo IgG como es comparado con los anticuerpos de isotipo IgM. Se proporcionan composiciones inmunogénicas que comprenden el material de reacción cruzada de toxina de difteria de proteína portadora 197 (DT-CRM 197) y el adyuvante C34. Los anticuerpos generados por las composiciones inmunogénicas divulgadas en la presente además neutralizan por lo menos uno de los antígenos Globo H, antígeno embriónico específico de etapa-3 (SSEA-3) y antígeno embriónico específico de etapa-4 (SSEA-4). Terapias contra células madre de cáncer de seno que comprenden composiciones inmunogénicas que comprenden Globo H, SSEA-3 o SSEA-4 conjugado con DT-CRM 197.