公开(公告)号：CA2976681A1

公开(公告)日：2016-09-01

申请号：CA2976681

申请日：2016-02-26

Applicant: FLUIDIGM CORP

Inventor： CONANT CAROLYN G ,  CHARN TZE HOWE ,  WEST JASON A A ,  WANG XIAOHUI

IPC: C40B20/00 ,  C12P19/34 ,  C12Q1/68 ,  C40B30/04 ,  C40B40/06 ,  C40B50/06

Abstract: Described herein are cell-based analytic methods, including a method of incorporating nucleic acid sequences into reaction products from a cell population, wherein the nucleic acid sequences are incorporated into the reaction products of each cell individually or in small groups of cells individually. Also described herein is a matrix-type microfluidic device that permits at least two reagents to be delivered separately to each cell or group of cells, as well as primer combinations useful in the method and device.

公开(公告)号：SG10201605049QA

公开(公告)日：2016-07-28

申请号：SG10201605049Q

申请日：2012-05-21

Applicant: FLUIDIGM CORP

Inventor： ANDERSON MEGAN ,  CHEN PEILIN ,  FOWLER BRIAN ,  JONES ROBERT C ,  KAPER FIONA ,  LEBOFSKY RONALD ,  ANDREW MAY

Abstract: Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.

公开(公告)号：CA2949952A1

公开(公告)日：2015-11-26

申请号：CA2949952

申请日：2015-05-21

Applicant: FLUIDIGM CORP

Inventor： XI LEI ,  WANG XIAOHUI ,  UNGER MARC ,  RUFF DAVID

IPC: C12Q1/68 ,  C12N15/09 ,  C12N15/10 ,  C12N15/63 ,  C12P19/34

Abstract: In certain embodiments, the present invention provides a way of "digitally" marking different the alleles of different chromosomes by using a transposase to insert differently barcoded transposons into genomic DNA before further analysis. According to this method, each allele becomes marked with a unique pattern of transposon barcodes. Because each unique pattern of transposon barcodes identifies a particular allele, the method facilitates determinations of ploidy and copy number variation, improves the ability to discriminate among homozygotes, heterozygotes, and patterns arising from sequencing errors, and allows loci separated by uninformative stretches of DNA to be identified as linked loci, thereby facilitating haplotype determinations. Also provided is a novel artificial transposon end that includes a barcode sequence in two or more positions that are not essential for transposition.

公开(公告)号：SG11201504876TA

公开(公告)日：2015-07-30

申请号：SG11201504876T

申请日：2014-03-14

Applicant: FLUIDIGM CORP

Inventor： WEST JASON A A ,  FOWLER BRIAN

IPC: C12M1/22

Abstract: Methods for cell analysis are provided, comprising cell capturing, characterization, transport, and culture. In an exemplary method individual cells (and/or cellular units) are flowed into a microfluidic channel, the channel is partitioned into a plurality of contiguous segments, capturing at least one cell in at least one segment. A characteristic of one or more captured cells is determined and the cell(s) and combinations of cells are transported to specified cell holding chamber(s) based on the determined characteristic(s). Also provided are devices and systems for cell analysis.

公开(公告)号：SG10201404683TA

公开(公告)日：2014-10-30

申请号：SG10201404683T

申请日：2009-12-07

Applicant: FLUIDIGM CORP

Inventor： FOWLER BRIAN

公开(公告)号：CA2895638A1

公开(公告)日：2014-09-18

申请号：CA2895638

申请日：2014-03-14

Applicant: FLUIDIGM CORP

Inventor： WEST JASON A A ,  FOWLER BRIAN

IPC: C12Q1/02 ,  C12M1/12 ,  C12M1/34 ,  C12N15/10 ,  C12Q1/00 ,  C12Q1/68

Abstract: Methods for cell analysis are provided, comprising cell capturing, characterization, transport, and culture. In an exemplary method individual cells (and/or cellular units) are flowed into a microfluidic channel, the channel is partitioned into a plurality of contiguous segments, capturing at least one cell in at least one segment, A characteristic of one or more captured cells is determined and the cell(s) and combinations of cells are transported to specified cell holding chamber(s) based on the determined characteristic(s). Also provided are devices and systems for cell analysis.

公开(公告)号：CA2698545C

公开(公告)日：2014-07-08

申请号：CA2698545

申请日：2008-09-08

Applicant: FLUIDIGM CORP

Inventor： RAMAKRISHNAN RAMESH

IPC: C12Q1/68

Abstract: The present invention methods and systems for determining copy number variation of a target polynucleotide in a genome of a subject including amplification based techniques. Methods can include pre-amplification of the sample followed by distribution of sample and a plurality of reaction volumes, quantitative detection of a target polynucleotide and a reference polynucleotide, and analysis so as to determine the relative copy number of the target polynucleotide sequence in the genome of the subject.

公开(公告)号：AU2008295992B2

公开(公告)日：2014-04-17

申请号：AU2008295992

申请日：2008-09-08

Applicant: FLUIDIGM CORP

Inventor： RAMAKRISHNAN RAMESH

IPC: G01N33/48

Abstract: The present invention methods and systems for determining copy number variation of a target polynucleotide in a genome of a subject including amplification based techniques. Methods can include pre-amplification of the sample followed by distribution of sample and a plurality of reaction volumes, quantitative detection of a target polynucleotide and a reference polynucleotide, and analysis so as to determine the relative copy number of the target polynucleotide sequence in the genome of the subject.

公开(公告)号：ES2380844T3

公开(公告)日：2012-05-18

申请号：ES08829141

申请日：2008-09-08

Applicant: FLUIDIGM CORP

Inventor： RAMAKRISHNAN RAMESH

IPC: C12Q1/68

Abstract: Un método para determinar el número de copias relativo de una secuencia polinucleotídica diana en el genoma de un sujeto, que comprende: preamplificar una secuencia polinucleotídica diana y una secuencia polinucleotídica de referencia en una muestra que contiene ADN genómico del sujeto; ensayar la secuencia polinucleotídica diana y la secuencia polinucleotídica de referencia de la muestra preamplificada mediante una PCR digital; determinar (a) el número de moléculas polinucleotídicas amplificadas que contienen la secuencia polinucleotídica diana, y (b) el número de moléculas polinucleotídicas amplificadas que contienen la secuencia polinucleotídica de referencia, y determinar la proporción de (a) a (b).

公开(公告)号：IL214034D0

公开(公告)日：2011-08-31

申请号：IL21403411

申请日：2011-07-12

Applicant: FLUIDIGM CORP

IPC: C12Q20060101