公开(公告)号：SG194722A1

公开(公告)日：2013-12-30

申请号：SG2013080858

申请日：2012-05-09

Applicant: FLUIDIGM CORP

Inventor： LIVAK KENNETH J ,  MYERS STACEY ,  WANG XIAOHUI ,  WANG JUN

公开(公告)号：EP3262214A4

公开(公告)日：2018-07-25

申请号：EP16756523

申请日：2016-02-26

Applicant: FLUIDIGM CORP

Inventor： CONANT CAROLYN G ,  CHARN TZE HOWE ,  WEST JASON A A ,  WANG XIAOHUI

IPC: C40B20/00 ,  B01L3/00 ,  C12N15/10 ,  C12P19/34 ,  C12Q1/68 ,  C12Q1/6844 ,  C40B30/04 ,  C40B40/06 ,  C40B50/06

CPC classification number: C12Q1/6874 ,  B01L3/5027 ,  C12Q1/6844 ,  C12Q2525/179 ,  C12Q2563/179 ,  C12Q2565/629

Abstract: Described herein are cell-based analytic methods, including a method of incorporating nucleic acid sequences into reaction products from a cell population, wherein the nucleic acid sequences are incorporated into the reaction products of each cell individually or in small groups of cells individually. Also described herein is a matrix-type microfluidic device that permits at least two reagents to be delivered separately to each cell or group of cells, as well as primer combinations useful in the method and device.

公开(公告)号：EP2707507A4

公开(公告)日：2015-03-18

申请号：EP12782232

申请日：2012-05-09

Applicant: FLUIDIGM CORP

Inventor： LIVAK KENNETH J ,  MYERS STACEY ,  WANG XIAOHUI ,  WANG JUN

IPC: C12Q1/68

CPC classification number: C12Q1/6818 ,  C12Q2525/151

公开(公告)号：CA2976681A1

公开(公告)日：2016-09-01

申请号：CA2976681

申请日：2016-02-26

Applicant: FLUIDIGM CORP

Inventor： CONANT CAROLYN G ,  CHARN TZE HOWE ,  WEST JASON A A ,  WANG XIAOHUI

IPC: C40B20/00 ,  C12P19/34 ,  C12Q1/68 ,  C40B30/04 ,  C40B40/06 ,  C40B50/06

Abstract: Described herein are cell-based analytic methods, including a method of incorporating nucleic acid sequences into reaction products from a cell population, wherein the nucleic acid sequences are incorporated into the reaction products of each cell individually or in small groups of cells individually. Also described herein is a matrix-type microfluidic device that permits at least two reagents to be delivered separately to each cell or group of cells, as well as primer combinations useful in the method and device.

公开(公告)号：CA2949952A1

公开(公告)日：2015-11-26

申请号：CA2949952

申请日：2015-05-21

Applicant: FLUIDIGM CORP

Inventor： XI LEI ,  WANG XIAOHUI ,  UNGER MARC ,  RUFF DAVID

IPC: C12Q1/68 ,  C12N15/09 ,  C12N15/10 ,  C12N15/63 ,  C12P19/34

Abstract: In certain embodiments, the present invention provides a way of "digitally" marking different the alleles of different chromosomes by using a transposase to insert differently barcoded transposons into genomic DNA before further analysis. According to this method, each allele becomes marked with a unique pattern of transposon barcodes. Because each unique pattern of transposon barcodes identifies a particular allele, the method facilitates determinations of ploidy and copy number variation, improves the ability to discriminate among homozygotes, heterozygotes, and patterns arising from sequencing errors, and allows loci separated by uninformative stretches of DNA to be identified as linked loci, thereby facilitating haplotype determinations. Also provided is a novel artificial transposon end that includes a barcode sequence in two or more positions that are not essential for transposition.

公开(公告)号：CA2949952C

公开(公告)日：2021-03-23

申请号：CA2949952

申请日：2015-05-21

Applicant: FLUIDIGM CORP

Inventor： XI LEI ,  WANG XIAOHUI ,  UNGER MARC ,  RUFF DAVID

IPC: C12N15/10 ,  C12N15/09 ,  C12N15/63 ,  C12P19/34

Abstract: In certain embodiments, the present invention provides a way of "digitally" marking different the alleles of different chromosomes by using a transposase to insert differently barcoded transposons into genomic DNA before further analysis. According to this method, each allele becomes marked with a unique pattern of transposon barcodes. Because each unique pattern of transposon barcodes identifies a particular allele, the method facilitates determinations of ploidy and copy number variation, improves the ability to discriminate among homozygotes, heterozygotes, and patterns arising from sequencing errors, and allows loci separated by uninformative stretches of DNA to be identified as linked loci, thereby facilitating haplotype determinations. Also provided is a novel artificial transposon end that includes a barcode sequence in two or more positions that are not essential for transposition.

公开(公告)号：SG11201706636PA

公开(公告)日：2017-09-28

申请号：SG11201706636P

申请日：2016-02-26

Applicant: FLUIDIGM CORP

Inventor： CONANT CAROLYN G ,  CHARN TZE HOWE ,  WEST JASON A A ,  WANG XIAOHUI

IPC: C40B20/00 ,  C12P19/34 ,  C12Q1/68 ,  C40B30/04 ,  C40B40/06 ,  C40B50/06

Abstract: Described herein are cell-based analytic methods, including a method of incorporating nucleic acid sequences into reaction products from a cell population, wherein the nucleic acid sequences are incorporated into the reaction products of each cell individually or in small groups of cells individually. Also described herein is a matrix-type microfluidic device that permits at least two reagents to be delivered separately to each cell or group of cells, as well as primer combinations useful in the method and device.