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公开(公告)号：WO2011142836A9
公开(公告)日：2012-01-05
申请号：PCT/US2011000887
申请日：2011-05-16
Applicant: FLUIDIGM CORP , JONES ROBERT C , LIVAK KENNETH J , MAY ANDREW , MIR ALAIN , PIEPRZYK MARTIN , QIN JIAN , RAMAKRISHNAN RAMESH , SPURGEON SANDRA , WANG JUN , ZIMMERMANN BERNHARD G
Inventor： JONES ROBERT C , LIVAK KENNETH J , MAY ANDREW , MIR ALAIN , PIEPRZYK MARTIN , QIN JIAN , RAMAKRISHNAN RAMESH , SPURGEON SANDRA , WANG JUN , ZIMMERMANN BERNHARD G
CPC classification number: C12Q1/6851 , C12Q1/6855 , C12Q1/6858 , G01N33/542 , G01N33/582 , G01N33/6893 , G01N2800/368 , C12Q2521/301 , C12Q2521/501 , C12Q2525/155 , C12Q2535/131 , C12Q2537/143 , C12Q2537/16 , C12Q2563/173
Abstract: The present invention provides amplification-based methods for detection of genotype, mutations, and/or aneuploidy. These methods have broad applicability, but are particularly well-suited to detecting and quantifying target nucleic acids in free fetal DNA present in a maternal bodily fluid sample.
Abstract translation: 本发明提供了用于检测基因型，突变和/或非整倍性的基于扩增的方法。 这些方法具有广泛的适用性，但特别适合于检测和定量存在于母体液样品中的游离胎儿DNA中的靶核酸。
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公开(公告)号：WO2010088288A2
公开(公告)日：2010-08-05
申请号：PCT/US2010022258
申请日：2010-01-27
Applicant: FLUIDIGM CORP , DUBE SIMANT , MIR ALAIN , RAMAKRISHNAN RAMESH , WEAVER LESLEY SUZANNE , ZIMMERMANN BERNHARD G
Inventor： DUBE SIMANT , MIR ALAIN , RAMAKRISHNAN RAMESH , WEAVER LESLEY SUZANNE , ZIMMERMANN BERNHARD G
CPC classification number: C12Q1/6853 , C12Q1/6809 , C12Q1/6886 , C12Q2600/16 , C12Q2561/113 , C12Q2545/10 , C12Q2527/107
Abstract: The present invention provides for determining relative copy number difference for one or more target nucleic acid sequences between a test sample and a reference sample or reference value derived therefrom. The methods facilitate the detection of copy number differences less than 1.5-fold.
Abstract translation: 本发明提供了确定测试样品和参考样品之间的一个或多个目标核酸序列的相对拷贝数差异或其衍生的参考值。 该方法有助于检测小于1.5倍的拷贝数差异。
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公开(公告)号：WO2011142836A3
公开(公告)日：2012-07-05
申请号：PCT/US2011000887
申请日：2011-05-16
Applicant: FLUIDIGM CORP , JONES ROBERT C , LIVAK KENNETH J , MAY ANDREW , MIR ALAIN , PIEPRZYK MARTIN , QIN JIAN , RAMAKRISHNAN RAMESH , SPURGEON SANDRA , WANG JUN , ZIMMERMANN BERNHARD G
Inventor： JONES ROBERT C , LIVAK KENNETH J , MAY ANDREW , MIR ALAIN , PIEPRZYK MARTIN , QIN JIAN , RAMAKRISHNAN RAMESH , SPURGEON SANDRA , WANG JUN , ZIMMERMANN BERNHARD G
CPC classification number: C12Q1/6851 , C12Q1/6855 , C12Q1/6858 , G01N33/542 , G01N33/582 , G01N33/6893 , G01N2800/368 , C12Q2521/301 , C12Q2521/501 , C12Q2525/155 , C12Q2535/131 , C12Q2537/143 , C12Q2537/16 , C12Q2563/173
Abstract: The present invention provides amplification-based methods for detection of genotype, mutations, and/or aneuploidy. These methods have broad applicability, but are particularly well-suited to detecting and quantifying target nucleic acids in free fetal DNA present in a maternal bodily fluid sample.
Abstract translation: 本发明提供用于检测基因型，突变和/或非整倍体的基于扩增的方法。 这些方法具有广泛的适用性，但是特别适合于检测和定量存在于母体体液样品中的游离胎儿DNA中的靶核酸。
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公开(公告)号：WO2010027870A3
公开(公告)日：2010-07-01
申请号：PCT/US2009055083
申请日：2009-08-26
Applicant: FLUIDIGM CORP , MIR ALAIN , RAMAKRISHNAN RAMESH , UNGER MARC , ZIMMERMANN BERNHARD G
Inventor： MIR ALAIN , RAMAKRISHNAN RAMESH , UNGER MARC , ZIMMERMANN BERNHARD G
CPC classification number: C12Q1/686 , C12N15/1065 , C12Q1/6853 , Y10T436/143333 , C12Q2537/143 , C12Q2525/161
Abstract: The present invention provides assay methods that increase the number of samples and/or target nucleic acids that can be analyzed in a single assay.
Abstract translation: 本发明提供了增加可以在单次测定中分析的样品和/或靶核酸数量的测定方法。
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公开(公告)号：WO2011143659A3
公开(公告)日：2012-07-12
申请号：PCT/US2011036670
申请日：2011-05-16
Applicant: FLUIDIGM CORP , MAY ANDREW , MIR ALAIN , RAMAKRISHNAN RAMESH , ZIMMERMANN BERNHARD
Inventor： MAY ANDREW , MIR ALAIN , RAMAKRISHNAN RAMESH , ZIMMERMANN BERNHARD
CPC classification number: C12P19/34 , C07H21/00 , C12Q1/6806 , C12Q1/6869 , C12Q2600/118 , C12Q2565/113 , C12Q2527/125 , C12Q2525/204 , C12Q2525/307 , C12Q2525/15 , C12Q2521/507
Abstract: The present invention provides methods for selectively enriching a biological sample for short nucleic acids, such as fetal DNA in a maternal sample or apoptic DNA in a biological sample from a cancer patient and for subsequently analyzing the short nucleic acids for genotype, mutation, and/or aneuploidy.
Abstract translation: 本发明提供了用于选择性富集来自癌症患者的生物样品中的母体样品中的短核酸例如胎儿DNA的生物样品或随机分析用于基因型，突变和/ 或非整倍体。
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Patent Agency Ranking
公开(公告)号：WO2008067552A3
公开(公告)日：2008-11-27
申请号：PCT/US2007086146
申请日：2007-11-30
Applicant: FLUIDIGM CORP , SUN GANG , RAMAKRISHNAN RAMESH , JONES ROBERT C
Inventor： SUN GANG , RAMAKRISHNAN RAMESH , JONES ROBERT C
CPC classification number: C12Q1/686 , C12Q2565/629 , C12Q2565/501 , C12Q2545/114
Abstract: A method of adjusting amplification curves in a PCR experiment includes receiving a plurality of amplification curves for a sample and computing a first parameter for each of the plurality of amplification curves. The method also includes computing a second parameter for each of the plurality of amplification curves and computing a third parameter using at least a portion of the first or second parameters. The method further includes computing an offset for each of the plurality of amplification curves. The offset is a function of the first parameter and the third parameter. Moreover, the method includes adjusting at least one of the plurality of amplification curves by subtracting the offset.
Abstract translation: 在PCR实验中调整扩增曲线的方法包括接收样品的多个扩增曲线并且计算多个扩增曲线中的每一个的第一参数。 该方法还包括为多个放大曲线中的每一个计算第二参数并且使用第一或第二参数的至少一部分来计算第三参数。 该方法还包括计算多个扩增曲线中的每一个的偏移。 偏移量是第一个参数和第三个参数的函数。 此外，该方法包括通过减去偏移来调整多个放大曲线中的至少一个。
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公开(公告)号：CA2734868A1
公开(公告)日：2010-03-11
申请号：CA2734868
申请日：2009-08-26
Applicant: FLUIDIGM CORP
Inventor： MIR ALAIN , RAMAKRISHNAN RAMESH , UNGER MARC , ZIMMERMANN BERNHARD G
Abstract: The present invention provides assay methods that increase the number of samples and/or target nucleic acids that can be analyzed in a single assay.
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公开(公告)号：EP2569453A4
公开(公告)日：2013-11-20
申请号：EP11781412
申请日：2011-05-16
Applicant: FLUIDIGM CORP
Inventor： MAY ANDREW , MIR ALAIN , RAMAKRISHNAN RAMESH , ZIMMERMANN BERNHARD
CPC classification number: C12P19/34 , C07H21/00 , C12Q1/6806 , C12Q1/6869 , C12Q2600/118 , C12Q2565/113 , C12Q2527/125 , C12Q2525/204 , C12Q2525/307 , C12Q2525/15 , C12Q2521/507
Abstract: The present invention provides methods for selectively enriching a biological sample for short nucleic acids, such as fetal DNA in a maternal sample or apoptic DNA in a biological sample from a cancer patient and for subsequently analyzing the short nucleic acids for genotype, mutation, and/or aneuploidy.
Abstract translation: 本发明提供了选择性富集生物样品的方法，所述生物样品用于短核酸，例如母体样品中的胎儿DNA或来自癌症患者的生物样品中的凋亡DNA，并随后分析短核酸的基因型，突变和/ 或非整倍体。
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公开(公告)号：EA018555B1
公开(公告)日：2013-08-30
申请号：EA201070349
申请日：2008-09-08
Applicant: FLUIDIGM CORP
Inventor： RAMAKRISHNAN RAMESH
IPC: G01N33/48
Abstract: Изобретениеотноситсяк способами системамдляопределениявариацииколичествакопийполинуклеотида-мишенив геномеиндивидуума, включающимоснованныенаамплификацииспособы. Способымогутвключатьпреамплификациюобразцас последующимраспределениемобразцавомножествореакционныхобъемов, количественноеопределениеполинуклеотида-мишении эталонногополинуклеотидаи анализдляопределенияотносительногоколичествакопийполинуклеотиднойпоследовательности-мишенив геномеиндивидуума.
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公开(公告)号：AT541946T
公开(公告)日：2012-02-15
申请号：AT08829141
申请日：2008-09-08
Applicant: FLUIDIGM CORP
Inventor： RAMAKRISHNAN RAMESH
IPC: C12Q1/68
Abstract: The present invention methods and systems for determining copy number variation of a target polynucleotide in a genome of a subject including amplification based techniques. Methods can include pre-amplification of the sample followed by distribution of sample and a plurality of reaction volumes, quantitative detection of a target polynucleotide and a reference polynucleotide, and analysis so as to determine the relative copy number of the target polynucleotide sequence in the genome of the subject.
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