Abstract:
A filter module (30) suitable for an optical instrument (A) for monitoring polymerase chain reaction replication of DNA in a reaction apparatus (B) that includes a thermal cycler block for holding a plurality of vials containing a suspension of ingredients for the reaction, the optical instrument including a housing suitable for receiving the filter module, the filter module comprising: a beam splitter (6) an excitation filter (7); an emission filter (8); and a support frame (34, 36, 40, 44); wherein: the beam splitter (6), the excitation filter (7) and the emission filter (8) are affixed to the support frame; the filter module (30) is suitable for being received in the housing of the optical instrument; the beam splitter (6), the excitation filter (7) and the emission filter (8), and thereby the filter module (30), are associated with a selected dye for the suspension; and the filter module (3) is removable from the housing of the optical instrument for replacement with another filter module associated with another selected dye.
Abstract:
An instrument includes a plurality of spaced-apart reaction regions (40) and a light source (10) comprising more than one individual light source. The light source is capable of illuminating more than one of the plurality of reaction regions with excitation beams (25). The individual light sources are arranged in groups and different groups of individual light sources are configured to emit different excitation frequencies. Each group of individual light sources is configured to provide excitation beams that illuminate one or more of the plurality of reaction regions simultaneously.
Abstract:
The compact microspectrometer for fluid media has, in a fixed spatial assignment in a housing (20b), a light source, a fluid passage (28b), a reflective optical grating (38b) and a detector (40b). The optical measurement path starting from the light source passes through the fluid passage and strikes the optical grating. The spectral light components which are reflected by the optical grating strike the detector.