公开(公告)号：KR1020080037805A

公开(公告)日：2008-05-02

申请号：KR1020060104909

申请日：2006-10-27

Applicant: 강원대학교산학협력단

Inventor： 이현용 ,  김정화 ,  김철희 ,  김효성 ,  권민철

IPC: A23L2/04 ,  A23L2/02 ,  A23L2/38

CPC classification number: A23L2/04 ,  A23L2/38 ,  A23V2002/00 ,  A23V2200/324 ,  A23V2250/21

Abstract: Rubus fruit extract having increased immune activity through ultrasonic extraction at low temperature and a fractionation stage is provided to exhibit higher immune activity and increased cell differentiation degree by an increase of differentiation-inducing material through multi-step differentiation. Rubus fruit is extracted in distilled water at 60deg.C for 24hr, ultrasonically extracted at 40KHz for 30min and fractionated to chloroform, ethyl acetate and butanol in order. For an example, Rubus fruit is washed, dried at 25 to 30deg.C in a shade and ground to 150meshes. The Rubus fruit powder is extracted in water at 100deg.C for 12hr, ultrasonically extracted at 40 KHz for 30min, filtered and concentrated under reduced pressure. The Rubus fruit concentrates are fractionated in steps containing chloroform, ethyl acetate and butanol in order, concentrated under reduced pressure and vacuum-dried.

Abstract translation: 通过在低温和分级分离阶段通过超声提取具有增加的免疫活性的鲁氏果实提取物通过多步分化增加分化诱导材料以显示更高的免疫活性和增加的细胞分化程度。 悬浮果实在60℃的蒸馏水中提取24小时，在40KHz下超声提取30分钟，分级分为氯仿，乙酸乙酯和丁醇。 例如，将胡子水果洗涤，在阴凉处25至30℃干燥并研磨至150目。 将芦荟果粉在100℃水中提取12小时，以40KHz超声提取30分钟，过滤并减压浓缩。 将Rubus果实浓缩物按步骤分批分级，依次加入氯仿，乙酸乙酯和丁醇，减压浓缩并真空干燥。

公开(公告)号：KR100981347B1

公开(公告)日：2010-09-10

申请号：KR1020070119968

申请日：2007-11-23

Applicant: 강원대학교산학협력단 ,  (주)스카이 007 ,  농업회사법인 주식회사 생명의나무

Inventor： 이현용 ,  권민철 ,  김철희 ,  곽형근 ,  나천수

IPC: B01D11/00

Abstract: 본 발명은 초음파 병행 기술을 이용한 효율적인 초고압 추출방법으로, 보다 상세하게는 통상적으로 100,000psi 이상에서 이루어지고 있는 초고압을 이용한 추출이 가진 위험하고, 비용이 많이 소요되며, 용매도 제한되는 단점을 개선하기 위하여 초고압추출에 선행하여 40~60kHz의 저에너지 초음파로 30~60분간 전처리함으로써 종래 초고압추출에 비하여 효율이 증진된 추출방법에 관한 것이다.
본 발명은 종래 초고압추출에 비하여 낮은 압력인 4,000~6,000atm에서 진행되어, 보다 낮은 압력 및 온도 조건에서보다 높은 수율을 가지는 추출방법으로, 에너지 효율이 높고, 다양한 용매의 사용이 가능하며, 높은 안정성을 가지고, 비용면에서도 경제적인, 초고압추출을 시행하는데 그 특징을 가진다.
본 발명의 초음파 병행을 통한 효율적 초고압 추출 기술을 이용하여 얻어진 추출물은 종래 통상적인 초고압추출의 추출물에 비해 작물의 유용 생리활성 물질에 대하여 높은 수율을 가짐으로써 우수한 효능을 가진다.

公开(公告)号：KR1020100033643A

公开(公告)日：2010-03-31

申请号：KR1020080092606

申请日：2008-09-22

Applicant: 조남현 ,  강원대학교산학협력단

Inventor： 이현용 ,  김철희 ,  김효성 ,  권민철 ,  한재건 ,  안주희 ,  조남현

IPC: C07K14/78 ,  C07K14/00 ,  A61K8/98

Abstract: PURPOSE: A method for whitened cuttlefish collagen-derived peptide is provided to optimize and simplify complex collagen isolation process and to improve skin absorption of the collagen. CONSTITUTION: A method for preparing whitened cuttlefish collagen-derived peptide comprises: a step of shaking a cuttlefish in sodium hydroxide solution to obtain cuttlefish collagen; a step of whitening collagen by shaking for 12 to 15 hours adding 1% of hydrogen peroxide; and a step of performing sonication of the whitened collage at 20, 60, 90 and 130KHz. The method further comprises: a step of putting 20 weight% of cuttlefish to 3% of hydrogen peroxide and 0.1N sodium hydroxide and shaking for 12 hours to remove non-collagen component, and a step of adding 0.5% of citric acid and vitamin C.

Abstract translation: 目的：提供一种美白的墨鱼胶原衍生肽的方法，以优化和简化复合胶原分离过程，并改善胶原蛋白的皮肤吸收。 构成：一种制备白蛋白鱼胶原衍生肽的方法，包括：在氢氧化钠溶液中摇动乌贼以获得墨鱼胶原蛋白的步骤; 振荡12〜15小时使胶原蛋白增白1％，加入1％过氧化氢的步骤; 并且在20,60,90和130KHz处进行白化拼贴的超声处理的步骤。 该方法还包括：将20重量％的墨鱼放入3％过氧化氢和0.1N氢氧化钠中，摇动12小时除去非胶原成分的步骤，加入0.5％柠檬酸和维生素C 。

公开(公告)号：KR1020090112444A

公开(公告)日：2009-10-28

申请号：KR1020080038346

申请日：2008-04-24

Applicant: 최영범 ,  강원대학교산학협력단

Inventor： 권민철 ,  이현용 ,  김철희 ,  한재건 ,  최영범 ,  고정림

IPC: B82B3/00 ,  B82Y5/00

Abstract: PURPOSE: A method for producing broccoli nanoparticle, and eatable composition containing the same are provided to prevent fast reaction after the destruction of tonoplast. CONSTITUTION: A method for producing broccoli nanoparticle comprises the following steps of: mixing broccoli, and water or ethanol 10 times amount of the broccoli and extracting at 50-60°C for 10-14 hours; irradiating ultrasonic wave of 40~120 kHz at 50-60°C for 0.5-3 hours to obtain broccoli ultrasonic wave extract; concentrating and freeze-drying the broccoli ultrasonic wave extract to obtain broccoli powder; dissolving the broccoli powder in purified water or water-soluble solvent to prepare a water-soluble broccoli extract; dissolving eatable oil phase selected among lecithin, gelatin, and chitosan in CH2Cl2 or chloroform solvent; evaporating the whole solvent to obtain liposome-like oil phase; pouring the water-soluble broccoli extract over the dried liposome-like oil phase; and dispersing the mixture in an ultrasonic disperser at 30-40°C for 50-60 minutes.

Abstract translation: 目的：提供一种生产西兰花纳米颗粒的方法，以及含有它们的可食用组合物，以防止在塑料破坏之后的快速反应。 构成：制备西兰花纳米颗粒的方法包括以下步骤：将西兰花和水或乙醇混合10倍量的西兰花，并在50-60℃下提取10-14小时; 在50-60℃下照射40〜120kHz的超声波0.5-3小时，得到西兰花超声波提取物; 浓缩冷冻干燥西兰花超声波提取物，得到西兰花粉; 将花椰菜粉末溶解在纯水或水溶性溶剂中以制备水溶性西兰花提取物; 将可溶性油相溶解在卵磷脂，明胶和壳聚糖中，在CH 2 Cl 2或氯仿溶剂中; 蒸发溶剂，得到脂质体样油相; 将水溶性西兰花提取物倒在干燥的脂质体样油相上; 并将混合物在超声波分散机中在30-40℃下分散50-60分钟。

公开(公告)号：KR1020090042349A

公开(公告)日：2009-04-30

申请号：KR1020070108056

申请日：2007-10-26

Applicant: 임태일 ,  양순복 ,  이명식 ,  강원대학교산학협력단

Inventor： 이현용 ,  권민철 ,  김철희 ,  임태일 ,  양순복 ,  이명식 ,  최영범

IPC: A23L1/16

CPC classification number: A23L7/109 ,  A21D2/36 ,  A23L27/10 ,  A23L33/105

Abstract: A method for manufacturing noodles containing an Opuntia ficus-indica is provided to improve quality for nutrition, texture, color sense of the noodles, and sensuousness using the Opuntia ficus-indica extract as water for dough. A method for manufacturing noodles containing an Opuntia ficus-indica comprises the following steps of: kneading mixed powder consisting of 72-95% of flour, 4-25 weight% of starch, 1-3 weight% of salt powder adding 30-40%(v/w) of the Opuntia ficus-indica extract on the basis of starch ingredients; aging dough at 5-10°C for 6-24 hours; and manufacturing the noodles using a noodle making machine. The Opuntia ficus-indica extract is manufactured by extracting the Opuntia ficus-indica with 10-15%(w/v) of water at 60-100°C for 12-24 hours.

Abstract translation: 提供了含有仙人掌猕猴桃的面条的制造方法，以提高营养，质地，面条的色泽，以及使用仙人掌榕提取物作为面团的水的感觉。 一种含有芸苔的面条的制造方法，包括以下步骤：将由72-95％的面粉，4-25重量％的淀粉，1-3重量％的盐粉组成的混合粉末混合，加入30-40％ （v / w）基于淀粉成分的仙人掌提取物; 在5-10℃老化面团6-24小时; 并使用面条机制造面条。 仙人掌榕提取物通过在60-100℃下用10-15％（w / v）的水提取12-24小时的仙人掌榕果制造。

公开(公告)号：KR100670612B1

公开(公告)日：2007-01-17

申请号：KR1020050083777

申请日：2005-09-08

Applicant: 강원대학교산학협력단

Inventor： 이현용 ,  김정화 ,  김철희 ,  유진현

IPC: A61K36/254 ,  A61K36/232 ,  A61K36/73

Abstract: A method for extracting herbs is provided to be able to obtain an herb extract containing many useful ingredients, and improve the extraction yield compared to other extraction method by performing a hot-water extraction, an ultrasonic wave extraction and a high hydraulic extraction in complex. The method comprises the steps of: (a) after adding solvent, the amount of which is 10 times that of herb, into the herb, hot-water extracting it at a temperature of 40-60 deg.C for 24 hours; (b) after extracting it obtained from the step(a) for 30 minutes using ultrasonic wave having a frequency of 20-40 kHz, freezing the extract under a temperature of -20 deg.C and crushing it; and (c) after extracting it obtained from the step(b) for 1-5 minutes under the pressure of 200-300 mPa using a high hydraulic extractor, filtering under reduced pressure, concentrating and freeze-drying it.

Abstract translation: 提供了一种提取草药的方法，通过进行热水提取，超声波提取和复合物中的高液相萃取，可以获得含有许多有用成分的草本提取物，并提高其提取率。 该方法包括以下步骤：（a）将加入量为草药10倍的溶剂加入草药中，将热水在40-60℃的温度下提取24小时; （b）使用频率为20〜40kHz的超声波从步骤（a）中提取30分钟后，在-20℃的温度下冷冻该提取液并进行粉碎; 和（c）使用高液压萃取器在200-300mPa的压力下将其从步骤（b）中提取得到1-5分钟后，在减压下过滤，浓缩并冷冻干燥。