monocytogenes 검출용 프라이머 세트, 이 프라이머 세트를 포함하는 Listeria
monocytogenes 검출용 조성물, 이를 포함하는 Listeria
monocytogenes 검출용 키트 및 이 조성물을 이용하여 Listeria
monocytogenes 를 정량적으로 분석하는 방법에 관한 것이다. 본 발명에서 제공하는 프라이머 세트를 이용하면 종래의 24~48시간의 오랜 배양 시간 후 동정하는 것에 비하여 매우 빠른 시간에 오염여부를 진단 할 수 있어 신속한 진단을 효과적으로 수행 할 수 있다. 또한 다양한 식품에 대하여 각각 정량용 표준곡선을 작성하여 이들 식품군에 L. monocytogene 가 검출 되었을 때 검체의 양이 어느정도 인지 계산 할 수 있는 정량적 검사가 가능하다. 또한, 본 발명에서 제공하는 프라이머 세트는 다른 균종 뿐만 아니라 동일한 리스테리아균으로부터 L. monocytogenes 를 감별 할 수 있는바 특이적인 검출 및 진단이 가능하다.
Abstract:
PURPOSE: A medium for maintainig and determining west nile is provided to reduce the exposure by high risk virus and to shorten the use of harmful reagents. CONSTITUTION: A medium for maintaining and detecting west nile contains alpha-MEM or DMEM containing 1-10 g/L of glucose and pH indicator. The pH indicator is phenol red. A potency assay method for detecting west nile comprises: a step of culturing west nile host cells and a sample in the medium; and a step of measuring the pH concentration of the medium to detecting west nile virus and tittering west nile in a sample. The host cells are monkey kidney cells.
Abstract:
본 발명은 우역 바이러스 (RPV), 가성우역 바이러스 (PPRV), 블루텅 바이러스 (BTV), 리프트 계곡열 바이러스 (RVFV)에 특이적인 서열번호 1내지 19의 다중 PCR 프라이머 세트, 이를 이용하여 상기 바이러스들을 동시에 검출할 수 있는 방법 및 키트를 제공하는 것이다. 본 발명의 방법은 한 번의 반응으로 상기 4종 질병을 동시에 진단할 수 있어, 노동력 및 진단 시간 절감 등 질병의 조기 진단이 가능하다. 리프트 계곡열 바이러스, 블루텅 바이러스, 우역 바이러스, 가성 우역 바이러스, 프라이머, 다중 RT-PCR법, 단일 튜브-다중 RT-PCR
Abstract:
본발명은리프트계곡열바이러스의재조합 N 단백질에대한단클론항체및 이를이용한경합적효소결합면역측정법에관한것이다. 보다자세하게는리프트계곡열바이러스의재조합 N 단백질에대한단클론항체, 이를생산하는하이브리도마및 재조합 N 단백질을항원으로사용하고혈청항체와 N 단백질에대한단일클론항체를동시에반응시키는경합적효소결합면역측정법을적용하여리프트계곡열바이러스항체를신속하게검출할수 있는진단방법에관한것이다.
Abstract:
PURPOSE: A method and kit for detecting rinderpest virus(RPV), peste-des-petits-ruminants virus(PPRV), blutongue virus(BTV), and rift valley fever virus(RVFV) is provided to diagnose four diseases with less labor. CONSTITUTION: A primer set for amplifying F gene of rinderpest virus(RPV) has a primer having base sequences of sequence numbers 1 and 2. A primer set for amplifying F gene of Peste-des-petits-ruminants virus(PPRV) has a primer having sequences of sequence numbrs 3-6. A primer set for amplifying VP3 gene of bluetongue virus(BTV) has a primer having sequences of sequence numbers 7-15. A primer set for amplifying NS gene of rift valley fever virus(VFV) has a primer having sequences of sequence numbers 16-19. A method for detecting the presence of virus comprise: a step of extracting RNA from a sample having virus; and a step of performing multiple RT-PCR.
Abstract:
PURPOSE: A competitive ELISA using a monoclonal antibody to a recombinant N protein is provided to shorten diagnosis time. CONSTITUTION: A competitive ELISA using recombinant N protein and monoclonal antibody 1-59 is used for diagnosing rift valley fever. A method for diagnosing rift valley fever comprises: a step of attaching a recombinant N protein antigen of rift valley fever virus on an ELISA plate; a step of removing the recombinant N protein antigen which is not attached on the plate; a step of competing 1-59 monoclonal antibodies to the recombinant N protein; and a step of reacting the secondary antibody to the monoclonal antibody conjugated with a target material.
Abstract:
본 발명은 바실러스 에스피 87 (KACC 91098) 및 이의 용도에 관한 것으로서, 상기 균주는 내산성, 내담즙성 및 생체 안전성이 인정되고, 병원성 세균 억제활성을 가지며, 장내에서 독립된 균총(Niche)으로서 미생물의 길항 작용을 통한 병원성 균총의 증식을 감소시켜 질병을 예방하는 효과가 있다.
Abstract:
The present invention relates to a method for identifying meat (beef, pork, chicken and duck) and a kit for the same and, more specifically, to a method for identifying meat (beef, pork, chicken and duck) capable of amplifying an area specifically present in genomic DNA of each meat species and quickly and precisely identifying meat by using a primer, which does not react to genomic DNA of meat species except for relevant species, and a kit for the same. Processed meat product pulverized to a point, where meat texture or the like cannot be distinguished, can be quickly and precisely identified as the meat is for beef, pork, chicken and duct by using the method for identifying meat of the present invention. The method for identifying meat of the present invention can be easily carried out by using the kit for the same of the present invention, so even nonprofessionals can quickly and precisely perform the work such as checking the raw material of meat product and the present invention is expected to be a great help to eradicate cheating raw material of the meat product or the like.
Abstract:
PURPOSE: A method for distinguishing and diagnosing west nile virus and Japanese encephalitis virus is provided to simultaneously test two kinds of Togaviridae and flaviviridae viruses. CONSTITUTION: A primer set for detecting west nile virus and Japanese encephalitis virus contains: a west nile-specific primer set having a sense primer of sequence numbers 1, 2, 3, and 4 and an antisense primer of sequence numbers 5, 6, 7, 8, and 9; and a Japanese encephalitis virus-specific primer set having a sense primer of sequence numbers 10 and 11 and an antisense primer of sequence numbers 12 and 13. A composition for detecting the west nile virus and Japanese encephalitis virus contains the primer sets. The composition is an RT-PCR kit.
Abstract:
PURPOSE: A single tube-multiplex RT-PCR for simultaneously detecting eastern equine encephalitis, western equine encephalitis, and venezuelan equine encephalitis viruses is provided to construct quick and efficient virus diagnosis system. CONSTITUTION: A single tube-multiplex RT-PCR for simultaneously detecting eastern equine encephalitis, western equine encephalitis, and venezuelan equine encephalitis viruses contains RT-PCR primer set, reaction buffer. DNA polymerase, and reverse transcriptase. A RT-PCR primer set contains primers of sequence numbers 1 and 2, sequence numbers 3 and 4, or sequence number 5 and 6.