-
公开(公告)号：KR101374190B1
公开(公告)日：2014-03-17
申请号：KR1020110036195
申请日：2011-04-19
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N5/0735 , C12N13/00
Abstract: 본 발명은 돼지배아줄기세포주, 및 단위생식(parthenogenesis)법을 이용하여 돼지 미성숙 난자로부터 배반포(blastocyst)를 생산하고, 생산된 배반포를 부화시켜 돼지배아줄기세포를 제조하는 방법에 관한 것으로서, 본 발명의 방법으로 돼지배아줄기세포를 보다 효율적으로 제조할 수 있다.
-
12.发明公开돼지배아줄기세포 및 돼지 미성숙 난자로부터 돼지배아줄기세포를 제조하는 방법 有权PORCINE EMBRYONIC STEM CELL及其制备PORCINE EMBRYONIC STEM细胞的方法由含有其的PORCINE IMMATOR OOCYTES
公开(公告)号：KR1020120118676A
公开(公告)日：2012-10-29
申请号：KR1020110036195
申请日：2011-04-19
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N5/0735 , C12N13/00
Abstract: PURPOSE: A manufacturing method of pig embryonic stem cells from the pig embryonic stem cells and pig immature ovum is provided to have pluripotency which is similar to pig embryonic stem cells originated from blastodermic vesicle. CONSTITUTION: A manufacturing method of pig embryonic stem cells from the pig embryonic stem cells and pig immature ovum comprises the following steps: maturing ovum by cultivating immature ovum of the pig; parthenogenesizing the matured ovum by activating by electrostimulation; cultivating and incubating the activated ovum with blastocyst; and cultivating inner cell mass originated from the incubated blastodermic vesicle and producing the ovum originated embryonic stem cell. The ovum in the second step is the first or the second ovum. The blastocyst cultivation in the third step is completed in a culture medium in which the fetal bovine serum (FBS) is included. The inner cell mass cultivation in the fourth step is completed in the culture medium including LIF, and BFGF or SCF.
Abstract translation: 目的：提供来自猪胚胎干细胞和未成熟猪的猪胚胎干细胞的制造方法，其具有与源自胚细胞胚泡的猪胚胎干细胞相似的多能性。 构成：猪胚胎干细胞和未成熟猪的胚胎干细胞的制造方法包括以下步骤：通过培养猪的未成熟卵来成熟卵; 通过电刺激使成熟的卵子成孤雌; 用胚泡培养和孵育活化的卵子; 并培养源自培养的胚泡囊泡的内细胞团，并产生卵子来源的胚胎干细胞。 第二步中的卵子是第一个或第二个卵子。 在第三步骤中的胚泡培养在包含胎牛血清（FBS）的培养基中完成。 在包含LIF和BFGF或SCF的培养基中完成第四步的内细胞培养。
-
公开(公告)号：KR1020110009626A
公开(公告)日：2011-01-28
申请号：KR1020100070014
申请日：2010-07-20
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: A23K10/18 , A23K20/195 , C12N1/20 , C12R1/225
Abstract: PURPOSE: A feed additive composition including lactobacillus reuteri is provided to improve the intestinal regulation effect and the enteropathogenic bacteria inhibition effect of animals. CONSTITUTION: A feed additive composition contains lactobacillus reuteri with the 16s rDNA including a base sequence of the sequence number 1. The lactobacillus reuteri has the acid resistance, the bile tolerance, and the intestine adhesion capacity. The feed additive is capable of inhibiting the intestinal proliferation of pathogenic bacteria.
Abstract translation: 目的：提供包括乳酸杆菌的饲料添加剂组合物，以改善肠道调节作用和动物肠道致病菌的抑菌作用。 构成：饲料添加剂组合物含有含有16s rDNA的乳酸杆菌，其含有序列号1的碱基序列。罗伊酸乳酸杆菌具有耐酸性，胆汁耐受性和肠粘连能力。 饲料添加剂能够抑制病原菌的肠道增殖。
-
公开(公告)号：KR102061896B1
公开(公告)日：2020-01-09
申请号：KR1020180143325
申请日：2018-11-20
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12Q1/70 , C12Q1/6818
-
-
Patent Agency Ranking
公开(公告)号：KR1020160091602A
公开(公告)日：2016-08-03
申请号：KR1020150011902
申请日：2015-01-26
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: 본발명은 (a) 베타-락탐계항생제에내성인균주및 상기항생제에감수성인균주를배양한후, 상기배양된균주를베타-락탐계항생제에노출시킨다음, 여과하는단계를포함하는전처리단계, 및 (b) 액체크로마토그래피(LC)에두 개의매스스펙트로미터(MS)가연결되어되어있는 LC-MS/MS를이용하여상기전처리된균주의베타-락탐계항생제에대한내성을측정하는단계를포함하는베타-락탐계항생제내성의분석방법에관한것이다.
Abstract translation: 本发明涉及一种分析β-内酰胺抗生素抗性的方法，包括：（a）预处理步骤，包括培养对β-内酰胺抗生素和对抗生素敏感的菌株的抗性菌株的步骤，将培养菌株 到β-内酰胺抗生素，以及过滤所得产物的步骤; 和（b）使用其中两个质谱仪（MS）连接到液相色谱（LC）的LC-MS / MS测量预处理菌株的β-内酰胺抗生素的抗性。 根据本发明，用于分析β-内酰胺抗生素抗性的方法可以量化抗生素耐药性，并且使用简单的预处理方法在2小时内测量抗性。
-
公开(公告)号：KR1020150051122A
公开(公告)日：2015-05-11
申请号：KR1020140024179
申请日：2014-02-28
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N5/0797 , C12N5/0735
CPC classification number: C12N5/0618
Abstract: 본발명은배아줄기세포로부터신경교전구세포, 성상세포및 희소돌기아교세포로의분화방법에관한것이다. 본발명에따른배아줄기세포로부터신경교전구세포, 성상세포및 희소돌기아교세포로의분화방법을이용할경우, 수득된신경교전구세포의지속적인배양이가능하며, 상기신경교전구세포는성상세포및 희소돌기아교세포로의분화가가능하여이와관련된독성물질의스크리닝및 독성평가에유용하게이용될수 있다.
Abstract translation: 本发明涉及诱导胚胎干细胞将其分化成胶质祖细胞，星形胶质细胞和少突胶质细胞的方法。 根据本发明的一个实施方案，该方法包括以下步骤：1）培养胚胎干细胞以形成胚状体; 2）将胚状体分离成单个细胞并培养分离的单细胞以诱导神经干细胞的产生; 3）另外培养神经干细胞，将培养的神经干细胞分离成单细胞，将分离的神经干细胞传代培养5〜40次。 当使用根据本发明的方法时，可以连续培养获得的胶质祖细胞并诱导胶质祖细胞分化为星形胶质细胞和少突胶质细胞，从而有效地用于筛选与细胞有关的毒性物质并评估毒性 。
-
公开(公告)号：KR1020130016790A
公开(公告)日：2013-02-19
申请号：KR1020110078907
申请日：2011-08-09
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N5/0775 , C12Q1/02
Abstract: PURPOSE: A method for preparing pluripotent stem cells from canine umbilical cord matrix is provided to express one or more differentiation markers(Oct-3/4, Sox-2, SSEA-4, Nanog). CONSTITUTION: A method for preparing umbilical cord matrix-derived mesenchymal stem cells comprises: a step of collecting an umbilical cord matrix and removing amnion from the umbilical cord matrix; a step of treating the umbilical cord matrix with trypsin/EDTA at 36-38°C for 30 minutes to one hour; a step of removing umbilical artery and umbilical vein from the umbilical cord matrix; a step of treating the umbilical cord matrix with collagenase at 36-38°C for 30 minutes to 2 hours and collecting single cells; and a step of culturing and collecting the pluripotent mesenchymal stem cells in a medium. The medium contains bFGF(Basic Fibroblast Growth Factor).
Abstract translation: 目的：提供从犬脐带基质制备多能干细胞的方法，以表达一种或多种分化标志物（Oct-3/4，Sox-2，SSEA-4，Nanog）。 构成：制备脐带基质的间充质干细胞的方法包括：收集脐带基质并从脐带基质中除去羊膜的步骤; 用胰蛋白酶/ EDTA在36-38℃处理脐带基质30分钟至1小时的步骤; 从脐带基质中去除脐动脉和脐静脉的步骤; 用胶原酶在36-38℃处理脐带基质30分钟至2小时并收集单细胞的步骤; 以及在培养基中培养和收集多能间充质干细胞的步骤。 培养基含有bFGF（碱性成纤维细胞生长因子）。
-
公开(公告)号：KR101201338B1
公开(公告)日：2012-11-15
申请号：KR1020100070014
申请日：2010-07-20
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: 본 발명은 내산성, 내담즙성, 장내부착능, 전분가수분해능이 우수하고, 항생제에 대한 감수성이 있으며, 동물이 섭취했을 때 증체량, 병원성 세균 방어효과 등의 우수한 효과를 나타내는 신규한 락토바실러스 루테리에 관한 것이다.
본 발명의 신규한 락토바실러스 루테리를 동물용 사료 또는 의약품으로 사용할 경우, 동물의 장내 병원성 세균 억제, 소화증진 및 정장효과를 지속적으로 나타낼 수 있어 축산 농가의 가축질병으로 인한 경제적 피해를 크게 감소시킬 수 있고, 뿐만 아니라 이를 활용하여 수입 동물용 의약품을 대체할 수 있다.-
20.发明授权아플라톡신 Ｂ1에 대한 결합 특이성 및 친화성이 향상된 단클론 항체 및 이를 포함하는 아플라톡신 Ｂ1 검출용 키트 有权单克隆抗体增强了与黄曲霉毒素B1的结合的特异性和亲和力，以及用于检测黄曲霉毒素B1的黄曲霉毒素B1的试剂盒
公开(公告)号：KR101149874B1
公开(公告)日：2012-06-27
申请号：KR1020090047325
申请日：2009-05-29
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: PURPOSE: A monoclonal antibody with improved binding specificity and affinity and a kit for detecting aflatoxin B1 are provided to enhance purification efficiency and detection intensity. CONSTITUTION: A conjugate comprises aflatoxin B1, a linker, and a carrier protein. The mole ratio of aflatoxin B1 and carrier protein is 1:5.5-1:6.5, respectively. The carrier protein is Bovine Serum Albumin(BSA), KLH(keyhole limpet hemocyanin), or ovalbumin. A kit for detecting aflatoxin B1 contains the monoclonal antibody.
-
-
-
-
-
-
-
-
-
Search Results
22
records
(took 0.02 seconds)
