Abstract:
본발명은클라비박터미시가넨시스아종네브라스켄시스를특이적으로검출하기위한프라이머세트및 이의용도에관한것으로, 본발명에서는총 5개의프라이머세트를이용하여검역세균인클라비박터미시가넨시스아종네브라스켄시스(subsp.)를특이적으로검출할수 있는것을확인하였다. 따라서, 본발명을통해클라비박터미시가넨시스아종네브라스켄시스에대한특이적검출이쉽고빠르게수행될수 있으므로, 클라비박터미시가넨시스아종네브라스켄시스균주의국내로의도입을철저히방지할수 있어, 매우유용하다.
Abstract:
The present invention relates to an oligonucleotide probe set to specifically diagnose or detect Tobacco necrosis virus (TNV), genechips comprising the oligonucleotide probe set to specifically diagnose and detect the TNV, a method to specifically diagnose or detect the TNV using the oligonucleotide probe set, and a kit comprising the oligonucleotide probe set and a hybridization solution to specifically diagnose or detect the TNV. Since the TNV is specifically detectable using the probe set of the present invention, the probe set is expected to be useful in increasing the production of domestic agricultural products by minimizing the viral diseases of the domestic agricultural products.
Abstract:
The present invention relates to a method for detecting and distinguishing a host-specific strain of a Tomato yellow leaf curl virus (TYLCV) using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). More specifically, the present invention relates to a method for detecting and distinguishing Korea, Israel, Spain, USA, Jordan 1, Jordan 2, and Jordan 3 which are seven strains of the Tomato yellow leaf curl virus (TYLCV) from an amplified product, which is amplified using a primer set for PCR for detecting a host-specific strain of a TYLCV, using Dde I, Fau I, or Bss SI which is a restriction enzyme. By means of the method according to the present invention, the infection of the TYLCV and the strain can be effectively confirmed within a short period of time without high-priced technical equipment such as sequence analysis equipment. Accordingly, the spread of the TYLCV is domestically prevented, and the inflow of domestically non-occurred strains can be prevented in a plant quarantine step, so that a huge economical loss can be remarkably reduced.