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11.发明公开느타리 버섯으로부터 분리된 신규한 ＧＰＤ유전자를 형질전환시킨 오스모틱 스트레스 내성 효모 有权由包含从PLEUROTUS SAJOR-CAJU分离的GPD基因的重组载体转化的耐摩擦重组酵母
公开(公告)号：KR1020010011673A
公开(公告)日：2001-02-15
申请号：KR1019990031152
申请日：1999-07-29
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/81
Abstract: PURPOSE: An osmotic stress resistant yeast transformed by a recombinant vector containing the GPD gene that isolated from Pleurotus sajor-caju is provided for improving the osmotic stress resistance of yeast. CONSTITUTION: The osmotic stress resistant yeast transformed by a recombinant vector containing glycerol-3-phosphate dehydrogenase(GPD) gene as an osmotic stress related gene is obtained by the following steps of: incubating Pleurotus sajor-caju ASI 2070 in an MCM medium containing 20g of peptone, 2g of yeast extract, 2g of K2HPO4, 1.0g of KH2PO4, 0.46g of MgSO47H2O, 0.5g of DW1L at 25 deg. C for 15 days; filtering with mira cloth; separating total RNA and DNA of Pleurotus sajor-caju ASI 2070 by using a Graham method and a Sambrook method, respectively; preparing the pYES2 vector containing the GPD gene by using restriction enzymes; and transforming Saccharomyces cerevisiae INVSC2 with the pYES2 vector.
Abstract translation: 目的：提供通过含有从侧耳属sajor-caju分离的GPD基因的重组载体转化的渗透胁迫酵母，用于改善酵母的渗透胁迫性。 构成：通过含有甘油-3-磷酸脱氢酶（GPD）基因的重组载体转化的渗透压胁迫酵母作为渗透胁迫相关基因，通过以下步骤获得：将Pleurotus sajor-caju ASI 2070在含有20g的MCM培养基中培养 的蛋白胨，2g酵母提取物，2g K 2 HPO 4，1.0g KH 2 PO 4，0.46g MgSO 4·7H 2 O，0.5g DW1L，25℃。 C 15天; 用米拉布过滤; 分别使用Graham方法和Sambrook方法分离了侧耳菇sajor-caju ASI 2070的总RNA和DNA; 通过使用限制酶制备含有GPD基因的pYES2载体; 并用pYES2载体转化酿酒酵母INVSC2。
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公开(公告)号：KR101791563B1
公开(公告)日：2017-11-02
申请号：KR1020150065251
申请日：2015-05-11
Applicant: 대한민국(농촌진흥청장)
Abstract: 본발명은특정음역대의음파처리에의한과실의성숙을지연시키는방법에관한것으로, 더욱구체적으로는음파처리한식물의과실은에틸렌생합성유전자의발현이억제되어과실의성숙이지연되었으므로, 수확후 농산물의선도유지및 장기저장에활용이가능한효과가있다.
Abstract translation: 本发明涉及通过某些eumyeokdae的声波治疗延缓果实成熟的方法，所述声波处理厂的更具体euroneun故障是因为果实的成熟yijiyeon，乙烯生物合成基因的表达被抑制，导致收获后的农产品 它可以用于维护和长期存储。
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公开(公告)号：KR1020160029979A
公开(公告)日：2016-03-16
申请号：KR1020140119270
申请日：2014-09-05
Applicant: 가천대학교 산학협력단 , 대한민국(농촌진흥청장)
CPC classification number: A61K31/065 , A23L29/035 , A23V2200/318 , A61K8/347 , A61K8/97 , A61K9/14 , A61K36/899 , A61K41/00 , A61K2121/00 , A61Q19/02
Abstract: 본발명은레스베라트롤을생산하는벼 유래캘러스로부터나노입자를제조하는방법, 상기방법으로제조된레스베라트롤생합성캘러스나노입자, 상기레스베라트롤생합성캘러스나노입자를포함하는, 피부색소침착예방또는치료용약학조성물, 피부색소침착예방또는개선용식품조성물, 피부색소침착예방또는개선용의약외품조성물및 피부미백용화장료조성물에관한것이다. 본발명에서제공하는레스베라트롤생합성캘러스나노입자는표피조직에대한투과성이우수할뿐만아니라, 향상된피부미백효과를나타내므로, 색소가침착된피부를회복시킬수 있는다양한제품의개발에널리활용될수 있을것이다.
Abstract translation: 本发明涉及一种由能够生产白藜芦醇的水稻衍生愈伤组织获得的纳米颗粒的制备方法以及通过该方法生产的白藜芦醇 - 生物合成愈伤组织纳米颗粒。 此外，本发明还涉及用于预防或治疗皮肤色素沉着的药物组合物，用于预防或改善皮肤色素沉着的食品组合物，用于预防或改善皮肤色素沉着的准药物组合物和美白化妆品组合物，其中全部 组合物含有白藜芦醇 - 生物合成愈伤组织纳米颗粒。 在本发明中提供的白藜芦醇 - 生物合成愈伤组织纳米颗粒对表皮组织具有优异的渗透性，并且表现出改善的皮肤美白效果。 因此，本发明的组合物可广泛用于开发能够修复着色皮肤的各种产品。
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公开(公告)号：KR101190272B1
公开(公告)日：2012-10-17
申请号：KR1020090049390
申请日：2009-06-04
Applicant: 대한민국(농촌진흥청장)
IPC: C07K14/425 , C12N15/29 , A01H1/00
Abstract: 본 발명은 벼 유래의 OsZIP1 단백질 및 이를 코딩하는 핵산 분자에 관한 것이다. 본 발명의 OsZIP1 핵산 분자를 이용하여 식물체를 형질전환시킴으로써, 병 저항성을 가지며, 야생형에 비하여 식물 개체의 크기 감소, 수술 길이의 단축, 꽃의 크기 축소 또는 화분 미형성의 생육 억제 양상 및 진한 화색의 표현형을 유도할 수 있으며, 이를 통해 식물(특히, 화훼작물)의 생산성 및 상품성(미니 꽃 생산)의 증대를 도모할 수 있다.
병 저항성, 벼, 담배, OsZIP1, 생육 억제-
公开(公告)号：KR1020100007772A
公开(公告)日：2010-01-22
申请号：KR1020090062798
申请日：2009-07-10
Applicant: 대한민국(농촌진흥청장)
Inventor： 김범기 , 변명옥 , 정미정 , 박수철 , 이성곤 , 권택륜 , 조우석 , 윤혜진 , 박상렬 , 이혜은 , 김둘이 , 신동진 , 이정원 , 한세연 , 권혁빈 , 문석준
Abstract: PURPOSE: An StMyb gene having salt-resistance or drought-resistance of plant is provided to use in developing plant species. CONSTITUTION: An StMyb(Solanum tuberosum Myb) polypeptide comprises an amino acid of sequence number 1. An StMyb polynucleotide has a gene sequence of sequence number 2. The intracellular increase of StMyb polypeptide is performed by transforming a plant with a vector containing the polynucleotide and a promoter. A method for producing the plant enhancing salt tolerance or drought tolerance comprises: a step of overexpressing StMyb polypeptide having amino acid of sequence number 1; and a step of transforming the plant with the expression vector.
Abstract translation: 目的：提供植物耐盐性或抗旱性的StMyb基因，用于开发植物物种。 构成：StMyb（Solanum tuberosum Myb）多肽包含序列号1的氨基酸.StMyb多核苷酸具有序列号2的基因序列。通过用含有多核苷酸的载体转化植物，进行StMyb多肽的细胞内增加， 一个启动子 用于产生植物增强耐盐性或耐旱性的方法包括：过表达具有序列号1的氨基酸的StMyb多肽的步骤; 和用表达载体转化植物的步骤。
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Patent Agency Ranking
公开(公告)号：KR100795421B1
公开(公告)日：2008-01-21
申请号：KR1020070031126
申请日：2007-03-29
Applicant: 대한민국(농촌진흥청장)
Abstract: A method for control of gray mold caused by Botrytis cinerea is provided to inhibit growth of Botrytis cinerea without specific equipments and technique by treating a plant with a single frequency sound, so that the gray mold is suppressed in an environment-friendly manner. The gray mold caused by Botrytis cinerea is controlled by treating a subject plant with a single frequency sound selected from 250-500 Hz generated by a sound generator, wherein the plant is vegetable including tomato, cucumber, strawberry, lettuce, eggplant and red pepper, or flower including lily, gladiolus, rose and carnation.
Abstract translation: 提供了一种灰葡萄孢灰霉菌控制方法，通过对具有单频声音的植物进行处理，无特殊设备和技术抑制灰葡萄孢的生长，从而以环保的方式抑制灰霉病。 灰葡萄孢造成的灰霉病是通过用声发生器产生的250-500Hz的单频声处理受试植物来控制的，其中植物是包括番茄，黄瓜，草莓，莴苣，茄子和红辣椒在内的蔬菜， 或花，包括百合，唐菖蒲，玫瑰和康乃馨。
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公开(公告)号：KR101528740B1
公开(公告)日：2015-06-15
申请号：KR1020130090470
申请日：2013-07-30
Applicant: 대한민국(농촌진흥청장) , 가천대학교 산학협력단
IPC: A61K36/899 , A61K8/97 , A61P17/00 , A61Q19/02
Abstract: 본발명은레스베라트롤생합성발아현미를유효성분으로하는조성물에관한것이다. 특히, 상기본 발명은멜라닌생성에관여하는효소인티로시나아제발현의억제활성이있어, 멜라닌생성을억제하는효과를가지는레스베라트롤생합성발아현미를유효성분으로포함하는피부색소침착예방또는치료용약학적조성물, 식품조성물, 의약외품조성물및 피부미백용화장료조성물에관한것이다. 본발명에따른레스베라트롤생합성발아현미를포함하는조성물은멜라닌생성에관여하는효소인티로시나아제발현의억제활성이있어, 멜라닌생성을억제함으로피부색소침착예방또는치료용약학적조성물, 식품조성물, 의약외품조성물및 피부미백용화장료조성물에유용하게사용될수 있다.
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公开(公告)号：KR101508745B1
公开(公告)日：2015-04-08
申请号：KR1020130072352
申请日：2013-06-24
Applicant: 대한민국(농촌진흥청장)
Abstract: 본발명은특정음역대의음파처리에의한식물의염 스트레스저항성증진방법에관한것으로, 더욱구체적으로는염 스트레스조건하에서, 음파처리한식물의수분및 양분흡수정도가양호하고및 광합성기구의손상이적으며, 음파처리한종자의염 스트레스조건하에서의발아율또한높았으므로염 스트레스에저항성을가지는작물및 종자제작에이용가능한효과가있다.
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公开(公告)号：KR1020120072477A
公开(公告)日：2012-07-04
申请号：KR1020100134242
申请日：2010-12-24
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A wound stress-induced promoter is provided to enhance the harvest amount of various crops and to produce commercial crops without environmental harm. CONSTITUTION: A wound stress-induced promoter derived from Oryza sativa has a base sequence of sequence number 1. The promoter contains a recombinant plant expression vector. The recombinant plant expression vector is prepared by operatively linking a target gene encoding a target protein at downstream of the promoter. The target protein is a plant disease resistant protein, a high functional protein, a cell regulatory protein, an immunomodulatory protein, an inhibitory protein, or an enzyme. A method for producing a transgenic plant comprises: a step of transforming plant cells with the recombinant plant expression vector; and a step of redifferentiating the transgenic plant.
Abstract translation: 目的：提供伤口应激诱导的促进剂，以增加各种作物的收获量，并生产无环境危害的农作物。 构成：来自水稻的伤口应激诱导的启动子具有序列号1的碱基序列。启动子含有重组植物表达载体。 通过在启动子的下游可操作地连接编码靶蛋白的靶基因来制备重组植物表达载体。 靶蛋白是植物病害抗性蛋白，高功能蛋白，细胞调节蛋白，免疫调节蛋白，抑制蛋白或酶。 一种生产转基因植物的方法包括：用重组植物表达载体转化植物细胞的步骤; 以及重新分化转基因植物的步骤。
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公开(公告)号：KR1020090121131A
公开(公告)日：2009-11-25
申请号：KR1020080047271
申请日：2008-05-21
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A broad host range disease resistance gene OsRBI1 is provided to promote expression of genes the enhance plant disease-resistance. CONSTITUTION: A plant has enhanced disease-resistance by improving intracellular level of polypeptide with an amino acid sequence of the sequence number 1. A method for producing a plant with enhanced disease-resistance comprises: a step of preparing an expression vector which overexpresses a polypeptide with the amino acid of the sequence number 1; and a step of transforming the plant with the expression vector. The expression vector comprises a promoter and a polynucleotide which is operably linked to the promoter and encodes the polypeptide of the sequence number 1.
Abstract translation: 目的：提供广泛的宿主范围抗病基因OsRBI1，以促进基因的表达，增强植物的抗病性。 构成：植物通过用序列号1的氨基酸序列改善多肽的细胞内水平来增强抗病性。一种具有增强的抗病性的植物的制备方法包括：制备过表达多肽的表达载体的步骤 与序列号1的氨基酸; 和用表达载体转化植物的步骤。 表达载体包含启动子和可操作地连接到启动子并编码序列号1的多肽的多核苷酸。
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