公开(公告)号：KR1019920009516B1

公开(公告)日：1992-10-17

申请号：KR1019900011101

申请日：1990-07-21

Applicant: 대한민국(농촌진흥청장)

Inventor： 황영수 ,  배신철 ,  최용문 ,  정태영 ,  김동수

IPC: C12Q1/68

Abstract: A DNA probe for early diagnosing black line dwarf disease virus (I) is prepd. by (a) separating the (I) from a green leafhopper larva infected with the (I), (b) preparing cDNA library of the (I), (c) ligating the obtd. cDNA with rambda gtll vector to obtain the recombinant rambda gtll phage DNA (II), (d) inserting the obtd. (II) into pUC19 and transforming into E. coli JM101, and (e) collecting the recombinant clone. The obtd. DNA probe is useful for the early diagnosis of a green leafhopper larba infected with the (I).

Abstract translation: 用于早期诊断黑线矮病病毒（I）的DNA探针是制备的。 通过（a）将（I）与（I）感染的绿叶蝉幼虫分离，（b）制备（I）的cDNA文库，（c）连接该物质。 具有λgtll载体的cDNA以获得重组噬菌体DNA（II），（d）插入该载体。 （II）转化成pUC19并转化到大肠杆菌JM101中，（e）收集重组克隆。 有限公司 DNA探针可用于早期诊断感染（I）的绿叶蝉幼虫。

公开(公告)号：KR100311094B1

公开(公告)日：2001-11-03

申请号：KR1019990031153

申请日：1999-07-29

Applicant: 대한민국(농촌진흥청장)

Inventor： 정미정 ,  박수철 ,  변명옥 ,  류진창 ,  정태영

IPC: C12N15/10

Abstract: 본발명은느타리버섯으로부터분리된 Osmotic stress 관련유전자인신규한글리세롤-3-포스페이트디하이드로지나제(이하 GPD라고약칭함)유전자와상기유전자를함유한재조합벡타를이용하여형질전환시킨감자및 그형질전환방법에관한것이다. 좀더 구체적으로는상기의 GPD 유전자를느타리버섯() ASI 2070 균주로부터분리하여이를식물형질전환용벡터인 pBI에클로닝한재조합벡터를이용하여감자를형질전환하는것으로서, 본발명에의하면기존의감자에비하여염, 저온, 고온및 건조등의외부환경적인스트레스에대한저항력이탁월하게증진되었고, 본발명에의한형질전환감자는특히내염성면에서바닷물의농도와비슷한 58dSm에서도생육하여염류집적이문제가되는간척지나비닐하우스에서도재배가가능한장점을지닌다. 한편상기유전자는식용버섯으로부터유래하였기때문에식품으로서유해성이없어산업상매우유용할것으로생각된다.

公开(公告)号：KR1019970007377B1

公开(公告)日：1997-05-08

申请号：KR1019930030237

申请日：1993-12-28

Applicant: 대한민국(농촌진흥청장)

Inventor： 박용환 ,  류진창 ,  정태영 ,  유영복 ,  정미정 ,  김범기 ,  이창수

IPC: C12Q1/68 ,  C12N15/00

Abstract: This invention is described about the method for discriminating of variety and quality of korean native cattle by the analysis of the difference of characteristic of DNA pleomorphism. Thus, DNA fragment which contains all or any one of base sequance AAGGTCCTAGGGTCCTGGG or eqivalent to this sequance by PCR reaction consists of DNA denaturing process, primer attaching process, DNA synthetic process contacts DNA of cattle at below 41 deg.C for 30-100 sec by using primer or probe, electrophoresis and detects to discriminate korean native cattle between holstein, hairford and aengus.

Abstract translation: 本发明描述了通过分析DNA多形性特征差异来区分韩国本地牛的品种和品质的方法。 因此，含有全部或任何一个碱基序列AAGGTCCTAGGGTCCTGGG或通过PCR反应等效于该序列的DNA片段由DNA变性过程，引物连接过程，DNA合成过程接触，在41摄氏度以下的牛的DNA接触30-100秒 通过使用引物或探针，进行电泳和检测，以区分霍尔斯坦，毛发和白菜之间的韩国本地牛。