公开(公告)号：KR1020100025655A

公开(公告)日：2010-03-10

申请号：KR1020080084291

申请日：2008-08-28

Applicant: 대한민국 (식품의약품안전처장)

Inventor： 엄소영 ,  정면우 ,  김규봉 ,  김선화 ,  최기환 ,  오혜영 ,  민홍기 ,  조명행

IPC: G01N33/50 ,  G01N33/15

Abstract: PURPOSE: A method for predicting side effects of non-steroidal anti-inflammation drug(NSAID) is provided to reduce time and cost without construction of separate database. CONSTITUTION: A method for predicting side effects of non-steroidal anti-inflammation drug(NSAID) comprises: a step of administering the NSAID to an experimental group of mammal except for human; a step of collecting urine 2 to 5 hours after administration and measuring the concentration of metabolite; and a step of comparing the concentration of the experimental group with a control group which a drug is not administered. The side effects are gastrointestinal function failure, peptic ulcer, heart or renal dysfunction. The metabolite is allantoin, taurine, acetate or benzoate.

Abstract translation: 目的：提供一种用于预测非甾体抗炎药（NSAID）的副作用的方法，以在不构建单独的数据库的情况下减少时间和成本。 构成：用于预测非甾体抗炎药物（NSAID）的副作用的方法包括：将除NSAID给予哺乳动物除了人之外的实验组的步骤; 给药后2〜5小时收集尿液并测定代谢物浓度的步骤; 以及将实验组的浓度与不施用药物的对照组进行比较的步骤。 副作用是胃肠功能衰竭，消化性溃疡，心脏或肾功能障碍。 代谢物是尿囊素，牛磺酸，乙酸盐或苯甲酸盐。

公开(公告)号：KR1020100060153A

公开(公告)日：2010-06-07

申请号：KR1020080118623

申请日：2008-11-27

Applicant: 대한민국 (식품의약품안전처장)

Inventor： 강태선 ,  우선욱 ,  박현주 ,  이윤숙 ,  민홍기

IPC: C12Q1/68

CPC classification number: C12Q1/6827 ,  C12Q2600/156 ,  C12Q2600/172

Abstract: PURPOSE: A method for analyzing the haplotype of CYP1A2 is provided to analyze genetic SNP of CYP1A2 and to develop a drug related to CYP1A2. CONSTITUTION: A method for analyzing the haplotype of CYP1A2 comprises: a step of obtaining a promoter, intron 4, intron 6, and exon 7 of a gene encoding CYP1A2 from a genome DNA; a step of detecting the presence of SNP(single nucleotide polymorphism); and a step of identifying the detected SNP to a haplotype selected from H1-H11.

Abstract translation: 目的：提供CYP1A2单倍型分析方法，分析CYP1A2基因SNP，开发CYP1A2相关药物。 构成：用于分析CYP1A2的单倍型的方法包括：从基因组DNA获得编码CYP1A2的基因的启动子，内含子4，内含子6和外显子7的步骤; 检测SNP（单核苷酸多态性）的存在的步骤; 以及将检测到的SNP鉴定为选自H1-H11的单倍型。

公开(公告)号：KR1020100060150A

公开(公告)日：2010-06-07

申请号：KR1020080118616

申请日：2008-11-27

Applicant: 대한민국 (식품의약품안전처장)

Inventor： 강태선 ,  우선욱 ,  박현주 ,  이윤숙 ,  민홍기

IPC: C12Q1/68

CPC classification number: C12Q1/6827 ,  C12Q2600/156 ,  C12Q2600/172

Abstract: PURPOSE: A method for analyzing the haplotype of a gene encoding CYP2E1 is provided to analyze SNP(single nucleotide polymorphism) and to develop drugs. CONSTITUTION: A method for analyzing CYP2E1 haplotype comprises: a step of obtaining a promoter, intron 5, intron 6, and exon 7 of a gene encoding CYP2E1 and determining nucleotide sequence thereof; a step of detecting presence of SNP; and a step of identifying the detected SNP. The SNP comprises a mutant in which promoter -1295 locus base G is replaced with base C, a mutant in which promoter -333 locus base T is replaced with base A, and a mutant a codon encoding phenylalanine base C is mutated with base T.

Abstract translation: 目的：提供一种分析编码CYP2E1基因单倍型的方法，分析SNP（单核苷酸多态性）和开发药物。 构成：分析CYP2E1单元型的方法，包括：获得编码CYP2E1的基因的启动子，内含子5，内含子6和外显子7的步骤，并确定其核苷酸序列; 检测SNP存在的步骤; 以及识别检测到的SNP的步骤。 SNP包括其中启动子-1295基因座G被碱基C替代的突变体，其中启动子-333基因座T被碱基A替代的突变体，并且编码苯丙氨酸碱基C的突变体编码苯丙氨酸碱基C用碱T突变

公开(公告)号：KR1020100041018A

公开(公告)日：2010-04-22

申请号：KR1020080099994

申请日：2008-10-13

Applicant: 대한민국 (식품의약품안전처장)

Inventor： 안준익 ,  최선옥 ,  정호상 ,  신희정 ,  고문정 ,  정혜주 ,  민홍기 ,  조명행

IPC: C12Q1/68

CPC classification number: G01N33/53 ,  C12Q1/6837 ,  C12Q2600/142

Abstract: PURPOSE: A method for determining food additive having hepatotoxicity is provided to effectively determine hepatotoxicity and develop safe food additive. CONSTITUTION: A method for determining food additive having hepatotoxicity comprises: a step of treating food additive to liver cell line and culturing; a step of isolating RNA from liver cell line and synthesizing cDNA; and a step of measuring hepatotoxicity-specific gene expression level and comparing with control liver cell line to determine presence of hepatotoxicity. The liver cell line is HepG2. The measurement is performed through Northern blot.

Abstract translation: 目的：提供一种确定具有肝毒性的食品添加剂的方法，以有效确定肝毒性并开发安全的食品添加剂。 构成：一种确定具有肝毒性的食品添加剂的方法，包括：对肝细胞系的食品添加剂进行处理和培养的步骤; 从肝细胞系中分离RNA并合成cDNA的步骤; 测定肝毒性特异性基因表达水平，与对照肝细胞系比较，测定肝毒性的存在。 肝细胞系为HepG2。 通过Northern印迹进行测量。

公开(公告)号：KR1020090083254A

公开(公告)日：2009-08-03

申请号：KR1020080009293

申请日：2008-01-29

Applicant: 대한민국 (식품의약품안전처장)

Inventor： 정이숙 ,  김미영 ,  서은지 ,  이동하 ,  조혜영 ,  정은용 ,  최기환 ,  김혜수 ,  민홍기

IPC: C12Q1/68 ,  C12N15/00

CPC classification number: G01N33/5008 ,  C12Q1/6844 ,  C12Q2600/136 ,  C12Q2600/158 ,  G01N2500/00

Abstract: A method for predicting the side effect of candidate drug on a cardiac myocyte is provided to develop a drug without side effect by easily verifying side effect of candidate drug. A method for predicting side effect of candidate drug on a heart comprises: a step of preparing an experimental group in which the candidate drug is treated to a cardiac myocyte and control group in which nothing is treated; a step of measuring expression of Gadd45beta gene in cardiac myocyte in experimental and control group; and a step of determining the possibility of damage.

Abstract translation: 提供了一种用于预测候选药物对心肌细胞的副作用的方法，通过容易地验证候选药物的副作用，开发出无副作用的药物。 用于预测候选药物对心脏的副作用的方法包括：制备将候选药物治疗至心肌细胞的实验组和不治疗任何物质的对照组的步骤; 在实验组和对照组中测量心肌细胞中Gadd45beta基因表达的步骤; 并确定损坏的可能性的步骤。