公开(公告)号：KR1020140119850A

公开(公告)日：2014-10-13

申请号：KR1020130032868

申请日：2013-03-27

Applicant: 전남대학교산학협력단

Inventor： 강경호 ,  강형일 ,  최석진 ,  허준욱 ,  정지섭 ,  박용빈 ,  오준영 ,  차준성 ,  홍혜란

IPC: C02F3/34 ,  C02F3/32

CPC classification number: C02F3/34 ,  C02F3/327 ,  Y02W10/15

Abstract: According to the present invention, microbes which live in Perinereis aibuhitensis in coastal mud flats have been grown in a MA culture medium, thus leading to the appearance of colonies, wherein the Perinereis aibuhitensis is known to have excellent degradability of organic matter. Thirty-one colonies have been separately selected according to the colors and shapes of the colonies which have appeared in the MA culture medium. Strains belonging to Bacillus genus have been isolated as a result of identification on the basis of 16S rRNA sequences after a pure culture process of the 31 colonies. Two Bacillus strains, CBW4 and EBW4, isolated from the Perinereis aibuhitensis have excellent degradabilities of protein, carbohydrate and lipid, and have resistance to salt. Accordingly, the CBW4 and EBW4 can be practically used to purify the water environment of a biofloc culture system contaminated with organic matter.

Abstract translation: 根据本发明，在沿海泥浆平面上生活在围Per中的微生物已经在MA培养基中生长，从而导致菌落的出现，其中已知百日蓟马具有优异的有机物的降解性。 根据出现在MA培养基中的菌落的颜色和形状分别选择了三十一个菌落。 在31个菌落的纯培养过程之后，基于16S rRNA序列的鉴定结果已经分离了属于芽孢杆菌属的菌株。 从百日蔷薇中分离出的两种芽孢杆菌属菌株CBW4和EBW4具有优异的蛋白质，碳水化合物和脂质的降解性，具有耐盐性。 因此，CBW4和EBW4可以实际应用于净化有机物污染的生物絮凝培养体系的水环境。

公开(公告)号：KR1020140119847A

公开(公告)日：2014-10-13

申请号：KR1020130032749

申请日：2013-03-27

Applicant: 전남대학교산학협력단

Inventor： 강경호 ,  강형일 ,  최석진 ,  허준욱 ,  정지섭 ,  김지건

IPC: C02F3/34

CPC classification number: C02F3/34 ,  Y02W10/15

Abstract: According to the present invention, microbes which live in Perinereis aibuhitensis in coastal mud flats have been grown in a MA culture medium, thus leading to the appearance of colonies, wherein the Perinereis aibuhitensis is knownto have excellent degradability of organic matter. Thirty-one colonies have been separately selected according to the colors and shapes of the colonies which have appeared in the MA culture medium. Strains belonging to Bacillus genus have been isolated as a result of identification on the basis of 16S rRNA sequences after a pure culture process of the 31 colonies. Two Bacillus strains, CBW4 and EBW4, isolated from the Perinereis aibuhitensis have excellent degradabilities of protein, carbohydrate and lipid, and have resistance to salt. Accordingly, the CBW4 and EBW4 can be practically used to purify the water environment of a biofloc culture system contaminated with organic matter.

Abstract translation: 根据本发明，在沿海泥浆平面上生活在围Per中的微生物已经在MA培养基中生长，从而导致菌落的出现，其中已知百日蓟马具有优异的有机物降解性。 根据出现在MA培养基中的菌落的颜色和形状分别选择了三十一个菌落。 在31个菌落的纯培养过程之后，基于16S rRNA序列的鉴定结果已经分离了属于芽孢杆菌属的菌株。 从百日蔷薇中分离出的两种芽孢杆菌属菌株CBW4和EBW4具有优异的蛋白质，碳水化合物和脂质的降解性，具有耐盐性。 因此，CBW4和EBW4可以实际应用于净化有机物污染的生物絮凝培养体系的水环境。

公开(公告)号：KR1020140119843A

公开(公告)日：2014-10-13

申请号：KR1020130032561

申请日：2013-03-27

Applicant: 전남대학교산학협력단

Inventor： 강경호 ,  강형일 ,  최석진 ,  허준욱 ,  정지섭 ,  김중근 ,  김지건 ,  김단오 ,  이준용

IPC: C02F3/34

CPC classification number: C02F3/34 ,  C02F2201/002 ,  Y02W10/15

Abstract: In the present invention, an enrichment culture is used to separate an EDB2 microorganism from mud, which excellently decomposes endosulfan and organic materials. A result of an analysis of the physical and biochemistry properties and genetic properties thereof shows the separated microorganism from the mud has a 99% similarity to Rhodococcus pvridinivorans.The separated microorganism is aerobic, positive to gram, a bar type and pink. The growth temperature range of the microorganism is relatively large, the growth temperature being 20-45°C, and the optimal temperature is 20-42°C. The microorganism can not hydrolyze casein and cellulose but can hydrolyze DNA, agar, starch and tween, which means the ability to decompose lipid is excellent. Accordingly the present invention can be used for purifying marine waste water contaminated by organic materials.

Abstract translation: 在本发明中，使用富集培养物将EDB2微生物与泥浆分离，其极好地分解硫丹和有机物质。 分析物理和生物化学性质及其遗传性质的结果表明，分离的微生物与泥土具有99％的相似性，与红球菌（Rhodococcus pvridinivorans）具有99％的相似性。分离的微生物是有氧的，对克，棒状和粉红色是正的。 微生物的生长温度范围较大，生长温度为20-45℃，最适温度为20-42℃。 微生物不能水解酪蛋白和纤维素，但可以水解DNA，琼脂，淀粉和吐温，这意味着分解脂质的能力非常好。 因此，本发明可用于净化被有机材料污染的海洋废水。

公开(公告)号：KR1020140073228A

公开(公告)日：2014-06-16

申请号：KR1020120141224

申请日：2012-12-06

Applicant: 전남대학교산학협력단

Inventor： 강형일 ,  강경호 ,  이상석

IPC: C12N1/20 ,  C12N9/16 ,  B09C1/10

Abstract: With respect to the present invention, microorganisms which are endogeneous in Perinereis aibuhitensis known to have excellent capacity to decompose organic matters in the coastal mud flat were grown in an MA medium, and 31 colonies were selected according to the color and shape of the colony and then purely cultured. As a result of identification based on the 16S rRNA nucleotide sequence, 11 strains included in the genus Bacillus were isolated. As a result of analysis of diversity of 11 strains included in the genus Bacills, it was investigated that a total of seven species of Bacills algicola, Bacills anthrasis, Bacills aquimaris, Bacills barbaricus, Bacills hwajinpoensis, Bacills nanhiensis, and Bacills vietnamensis are endogenous in Perinereis aibuhitensis. In the present invention, two Bacillus strains isolated and selected from Perinereis aibuhitensis have excellent capacity to decompose protein, hydrocarbon, and lipid, and have resistance to salt, and thus can be useful to purify a mud flat contaminated with organic matters.

Abstract translation: 对于本发明，在MA培养基中生长已知具有优异的分解沿海泥浆中的有机物质能力的围is it中的内分泌微生物，并根据菌落的颜色和形状选择31个菌落， 然后纯文化。 作为基于16S rRNA核苷酸序列的鉴定的结果，分离了芽孢杆菌属中包含的11个菌株。 通过分析芽孢杆菌属11种菌株的多样性，研究了七种芽孢杆菌，芽孢杆菌，芽孢杆菌，芽孢杆菌，百日咳芽孢杆菌，百日咳芽孢杆菌和越桔芽孢杆菌共有七种内源性 Per。 在本发明中，分离并选自百日香蓟马的两种芽孢杆菌菌株具有分解蛋白质，烃和脂质并具有耐盐性的优异能力，因此可用于纯化被有机物污染的泥浆。

公开(公告)号：KR1020140062949A

公开(公告)日：2014-05-27

申请号：KR1020120129282

申请日：2012-11-15

Applicant: 전남대학교산학협력단

Inventor： 강형일 ,  강경호 ,  이상석

IPC: C12N1/20 ,  B09C1/10

CPC classification number: C12N1/20 ,  B09C1/10 ,  C12R1/07

Abstract: In the present invention, a microorganism that inhabits a lugworm ( Perinereis aibuhitensis ) that is known for excellent organic material decomposition in shore mud flats is grown in an MA culture medium so that colonies occur. Thirty-one colonies are separately selected by color and shape in order to be purely cultured and identified based on a 16S rRNA base sequence, so that eleven strains included in the Bacills genus are separated. As a result of analyzing the variety of the eleven strains included in the Bacills genus, it has been investigated that seven Bacillus species, such as Bacills algicola, Bacills anthrasis, Bacills aquimaris, Bacills barbaricus, Bacills hwajinpoensis, Bacills nanhiensis, and Bacills vietnamensis , inhabit the lugworm ( Perinereis aibuhitensis ). In the present invention, two Bacillus strains that have been separated and selected from the lugworm have excellent protein, carbohydrate, and lipid decomposition and salinity resistance so as to be usefully used to purify a mud flat environment that is contaminated by organic material.

Abstract translation: 在本发明中，在MA培养基中生长以在岸边泥浆平面中优异的有机物质分解为特征的居住在具有优良有机物分解的蛔虫（Perinereis aibuhitensis））的微生物，从而发生菌落。 通过颜色和形状分别选择三十一个菌落，以便基于16S rRNA碱基序列纯化培养和鉴定，从而分离包含在11个菌属中的11个菌株。 作为分析“芽孢杆菌”中包含的十一个菌株的多样性的结果，已经研究了七种芽孢杆菌属物种，例如细菌藻类，芽孢杆菌性溃疡 ，百日咳杆菌（Bacills aquimaris），芽孢杆菌（Bacills barbaricus），百日咳芽孢杆菌（Bacills hwajinpoensis），百日咳芽孢杆菌（Bacills nanhiensis）和越南小菜蛾（Bacills vietnamensis）居住在蛔虫（ Perinereis aibuhitensis ）。 在本发明中，已经从蛔虫中分离和选择的两种芽孢杆菌菌株具有优异的蛋白质，碳水化合物和脂质分解和耐盐性，从而有用地用于纯化平坦的 被有机材料污染。