公开(公告)号：BRPI1012635A2

公开(公告)日：2016-06-21

申请号：BRPI1012635

申请日：2010-03-26

Applicant: ACADEMIA SINICA

Inventor： MA CHE ,  WANG CHI-CHENG ,  WONG CHI-HUEY ,  CHEN JUINE-RUEY

IPC: A61K39/12

公开(公告)号：AU2016203431A1

公开(公告)日：2016-06-16

申请号：AU2016203431

申请日：2016-05-25

Applicant: ACADEMIA SINICA

Inventor： WONG CHI-HUEY ,  MA CHE ,  WANG CHENG-CHI ,  CHEN JUINE-RUEY

IPC: A61K39/12

Abstract: METHODS AND COMPOSITIONS FOR IMMUNIZATION AGAINST Immunogenic compositions comprising partially glycosylated viral glycoproteins for use as vaccines against viruses are provided. Vaccines formulated using mono-, di-, or tri-glycosylated viral surface glycoproteins and polypeptides provide potent and broad protection against viruses, even across strains. Pharmaceutical compositions comprising monoglycosylated hemagglutinin polypeptides and vaccines generated therefrom and methods of their use for prophylaxis or treatment of viral infections are disclosed. Methods and compositions are disclosed for influenza virus HA, NA and M2, RSV proteins F, G and SH, Dengue virus glycoproteins M or E, hepatitis C virus glycoprotein El or E2 and HIV glycoproteins gp120 and gp4l.

公开(公告)号：CA3147075A1

公开(公告)日：2012-05-10

申请号：CA3147075

申请日：2011-11-04

Applicant: ACADEMIA SINICA

Inventor： WONG CHI-HUEY ,  MA CHE ,  TSENG YUNG-CHIEH

IPC: C12N7/00 ,  C07K1/14 ,  C07K14/11 ,  C12N7/02 ,  C12N7/04 ,  C12P21/02

Abstract: Methods for production of virus particles with simplified glycosylation on structural or surface proteins are provided. When used as targets for vaccine production, the conserved nature of such sites generates vaccines that are less sensitive to viral mutations. Use of glycosylation inhibitors for production of viruses with simplified glycosylation profiles are disclosed. An exemplary disclosure of influenza viruses and methods for production of mono-glycosylated influenza virus particles is provided. Methods for production of mono-glycosylated forms of influenza A virus, NIBRG-14 (H5N1) are provided.

公开(公告)号：AU2021268212A1

公开(公告)日：2022-12-08

申请号：AU2021268212

申请日：2021-05-07

Applicant: ACADEMIA SINICA

Inventor： LIAO HSIN-YU ,  WANG SHIH-CHI ,  KO YI-AN ,  LIN KUO-I ,  MA CHE ,  CHENG TING-JEN

IPC: A61K38/16 ,  A61K39/155 ,  C12N7/04

Abstract: The present disclosure relates to a chimeric influenza virus hemagglutinin (HA) polypeptide, comprising one or more stem domain sequence, each having at least 60% homology with a stem domain consensus sequence of HI subtype HA (HI HA) and/or H5 subtype HA (H5 HA), fused with one or more globular head domain sequence, each having at least 60% homology with a globular head domain consensus sequence of HI subtype HA (HI HA) or H5 subtype HA (H5 HA).

公开(公告)号：BR112013011198B1

公开(公告)日：2022-03-15

申请号：BR112013011198

申请日：2011-11-04

Applicant: ACADEMIA SINICA

Inventor： MA CHE ,  WONG CHI-HUEY ,  TSENG YUNG-CHIEH

IPC: C12N15/09 ,  C12P21/06

Abstract: métodos para produção de partículas virais com glicosilação simplificada de proteínas de superfície. métodos para produção de partículas virais com glicosilação simplificada em proteínas estruturais ou de superfície são fornecidos. quando usadas como alvos para produção de vacinas, a natureza conservada de tais sítios gera vacinas que são menos sensíveis a mutações virais. é discutido o uso de inibidores de glicosilação para produção de vírus com perfis de glicosilação simplificada. são fornecidos uma descrição ilustrativa do vírus da influenza e métodos para a produção de partículas do vírus da influenza monoglicosiladas. são fornecidos métodos para a produção de formas monoglicosiladas do vírus da influenza a, nibrg-14 (h5nl).

公开(公告)号：ES2831256T3

公开(公告)日：2021-06-08

申请号：ES11838917

申请日：2011-11-04

Applicant: ACADEMIA SINICA

Inventor： WONG CHI-HUEY ,  MA CHE ,  TSENG YUNG-CHIEH

IPC: C12P21/06 ,  A61K39/145 ,  C12N7/00 ,  C12N15/09 ,  C12P21/00

Abstract: Un método para producir un virus de la gripe desglicosilado que comprende un antígeno hemaglutinina (HA) y que tiene un solo resto de GlcNAc unido a un sitio de N-glicosilación, comprendiendo el método: a) cultivar un virus de la gripe que comprende un antígeno hemaglutinina (HA) en un hospedante adecuado en presencia de una cantidad eficaz de un inhibidor de a-manosidasa I, para producir glicoproteínas Man9(GlcNAc)2, en el que el hospedante adecuado es un huevo de gallina embrionado libre de patógenos específicos (SPF); b) recuperar el virus de la gripe producido en a); c) poner en contacto el virus de la gripe recuperado en b) con una endoglicosidasa (Endo H) para producir dicho virus de la gripe desglicosilado; y d) aislar el virus de la gripe desglicosilado.

公开(公告)号：ES2784189T3

公开(公告)日：2020-09-23

申请号：ES10756977

申请日：2010-03-26

Applicant: ACADEMIA SINICA

Inventor： WONG CHI-HUEY ,  MA CHE ,  WANG CHENG-CHI ,  CHEN JUINE-RUEY

IPC: A61K39/12 ,  A61K39/145

Abstract: Una composición inmunogénica que comprende: una glicoproteína hemaglutinina (HA) del virus de la gripe parcialmente glicosilada, en donde la glicoproteína HA comprende uno o más sitios de glicosilación a los que está unido un glicano, y en donde el glicano consiste en un residuo azúcar, dos residuos azúcar o tres residuos azúcar.

公开(公告)号：AU2016203431B2

公开(公告)日：2018-03-08

申请号：AU2016203431

申请日：2016-05-25

Applicant: ACADEMIA SINICA

Inventor： WONG CHI-HUEY ,  MA CHE ,  WANG CHENG-CHI ,  CHEN JUINE-RUEY

IPC: A61K39/12

Abstract: METHODS AND COMPOSITIONS FOR IMMUNIZATION AGAINST Immunogenic compositions comprising partially glycosylated viral glycoproteins for use as vaccines against viruses are provided. Vaccines formulated using mono-, di-, or tri-glycosylated viral surface glycoproteins and polypeptides provide potent and broad protection against viruses, even across strains. Pharmaceutical compositions comprising monoglycosylated hemagglutinin polypeptides and vaccines generated therefrom and methods of their use for prophylaxis or treatment of viral infections are disclosed. Methods and compositions are disclosed for influenza virus HA, NA and M2, RSV proteins F, G and SH, Dengue virus glycoproteins M or E, hepatitis C virus glycoprotein El or E2 and HIV glycoproteins gp120 and gp4l.

公开(公告)号：CA2973886A1

公开(公告)日：2016-08-04

申请号：CA2973886

申请日：2016-01-30

Applicant: ACADEMIA SINICA

Inventor： WONG CHI-HUEY ,  WU CHUNG-YI ,  MA CHE ,  WU HAN-CHUNG

IPC: C07K16/00 ,  A61K39/42 ,  C07K16/10 ,  C07K16/24 ,  C07K16/28 ,  C07K16/30

Abstract: The present disclosure relates to compositions and methods of use comprising antibodies or binding fragments thereof further comprising universal Fc glycoforms.

公开(公告)号：AU2010229675B2

公开(公告)日：2016-02-25

申请号：AU2010229675

申请日：2010-03-26

Applicant: ACADEMIA SINICA

Inventor： WONG CHI-HUEY ,  MA CHE ,  WANG CHENG-CHI ,  CHEN JUINE-RUEY

IPC: A61K39/12

Abstract: Immunogenic compositions comprising partially glycosylated viral glycoproteins for use as vaccines against viruses are provided. Vaccines formulated using mono-, di-, or tri-glycosylated viral surface glycoproteins and polypeptides provide potent and broad protection against viruses, even across strains. Pharmaceutical compositions comprising monoglycosylated hemagglutinin polypeptides and vaccines generated therefrom and methods of their use for prophylaxis or treatment of viral infections are disclosed. Methods and compositions are disclosed for influenza virus HA, NA and M2, RSV proteins F, G and SH, Dengue virus glycoproteins M or E, hepatitis C virus glycoprotein E1 or E2 and HIV glycoproteins gp120 and gp41.