12.
    发明专利
    未知

    公开(公告)号:DE50107447D1

    公开(公告)日:2005-10-20

    申请号:DE50107447

    申请日:2001-09-17

    Applicant: BASF AG

    Inventor: FRIEDRICH THOMAS

    New butynol I esterase, for stereospecific hydrolysis or transesterification of esters, to produce optically active reaction products

    公开(公告)号:DE10131544A1

    公开(公告)日:2003-01-16

    申请号:DE10131544

    申请日:2001-06-29

    Applicant: BASF AG

    Abstract: A protein (I) with butynol I esterase (BIE) activity comprising at least one of 4 partial sequence polypeptides (S1-4) and their functional equivalents, is new. A protein (I) with butynol I esterase (BIE) activity comprises at least one of 4 partial sequence polypeptides (S1-4) and their functional equivalents. Independent claims are also included for the following: (1) polynucleotides (II) that encode (I), and complementary polynucleotides and sequences that hybridize to them; (2) an expression cassette (EC) comprising at least one (II), operably linked to regulators; (3) a recombinant vector for transforming eukaryotic or prokaryotic hosts, containing (II) or EC; (4) preparation of (I) by culturing organisms that produce (I), either endogenously or after transformation; (5) (I) produced by the method in (4); Pseudomonas glumae Lu2023 (DSM 13176), or its variants or mutants; (6) microorganisms containing the vector of (4); (7) method for enantioselective ester hydrolysis or transesterification using (I); and (8) optically active alcohols, carboxylic acids or esters prepared using (I). Phe-Ile-Glu-Thr-Leu-Gly-Leu-Glu-Arg-Pro-Val-Leu-Val-Gly-His-Ser-Leu-Gl y-Gly-Ala-Ile-Ala-Leu-Ala-Val-Gly-Leu-Asp-Tyr-Pro-Glu-Arg (S1) Ile-Ala-Leu-Ile-Ala-Pro-Leu-Thr-His-Thr-Glu-Thr-Glu-Pro (S2) Gly-Gly-Gly-Met-Met-Gly-Leu-Arg-Pro-Glu-Ala-Phe-Tyr-Ala-Ala-Ser-Ser-As p-Leu-Val (S3) Ala-Ile-Asp-Ala-Ile-Phe-Ala-Pro-Glu-Pro-Val (S4)

    19.
    发明专利
    未知

    公开(公告)号:EE200100232A

    公开(公告)日:2002-08-15

    申请号:EEP200100232

    申请日:1999-10-13

    Applicant: BASF AG

    Abstract: The invention relates to nucleic acid sequences which code for a polypeptide having nitrilase activity, to nucleic acid constructs comprising the nucleic acid sequences, and to vectors comprising the nucleic acid sequences or the nucleic acid constructs. The invention further relates to amino acid sequences which are encoded by the nucleic acid sequences, and to microorganisms comprising the nucleic acid sequences, the nucleic acid constructs or vectors comprising the nucleic acid sequences or the nucleic acid constructs.The invention additionally relates to a process for preparing chiral carboxylic acids from the racemic nitriles.

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